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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Micropropagation of ten weeks (Matth iola incana) and lisianthus (Eustoma grandiflorum) (two ornamental plants) by using kinetin (KIN), naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D)

100%
Kaviani B.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2014
|
tom 13
|
nr 1
Micropropagation is a powerful tool for large-scale propagation of pathogenfree ornamental plants. Studies on micropropagation of ten weeks and lisianthus is relatively low, thus, here we present a reliable method for in vitro propagation of these two ornamental plants. The shoot tips explants from Eustoma grandiflorum and Matthiola incana were cultured on MS medium supplemented with concentrations of 0, 0.5, 1 and 2 mg·L of naphthalene acetic acid (NAA) and kinetin (KIN). In Eustoma grandiflorum, multiple shoots containing roots can be obtained simultaneously on MS basal medium only supplemented with 0.5–1 mg·L KIN. Shoot tips media supplemented with 1 mg· L KIN without NAA resulted in the best shoot length (2.158 cm) and shoot number (2.68). Also, the most nodes (8.75) were obtained in medium containing 0.5 mg·L KIN without NAA. The highest root number (2.55) was seen in medium supplemented with 2 mg·L KIN + 0.5 mg·L NAA. Shoot tips grown in medium containing 2 mg·L NAA without KIN showed the most callus formation. The highest content of fresh weight, dry weight and chlorophyll content were calculated in plantlets grown on media containing 0.5 mg·L KIN without NAA, 0.5 mg·L KIN + 1 mg·L NAA and control, respectively. In Matthiola incana, four-week-old in vitro plants obtained from micro-cuttings showed successful shooting and rooting. MS medium supplemented with 2 mg·L KIN without NAA resulted in the best shoot length (1.166 cm) and largest number of node (4.64). When the shoot tips were inoculated in the medium containing 2 mg·L NAA without KIN and medium containing the combination of 1 mg·L NAA + 2 mg·L KIN, the best result was observed for root number (1.85) and root length (5.2 cm).
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Effect of ascorbic acid concentration on structural characteristics of apical meristems on in vitro Aloe barbadensis Mill.

100%
Kaviani B.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2014
|
tom 13
|
nr 3
Ascorbic acid is one of the major metabolite in higher plants cells which is known as effective factor when the cells enter to “S” phase from “G1” phase of cytokinesis. This metabolite has antioxidant activity and increases plant tolerance against stressors such as salinity, pathogens, ozone, UV rays, etc. The current study used the common cellular and histological methods to evaluate the effect of 0.05 to 2.5 mM ascorbic acid on vegetative meristems of Aloe barbadensis plants obtained from stem explants propagated in vitro culture conditions. Results showed that low concentrations of ascorbic acid (0.5 to 1 mM) increase mitotic index in apical meristem and root quiescent center (QC). Moreover, treatment with ascorbic acid increases cellular dimensions in cell elongation region of root and mitotic divisions in this region. In some measurements, it was clear that in addition to increase root length in plants treated with ascorbic acid, distance from root hairs zone to root cap increases compared to the control, which is a logical conclusion from increasing cell elongation and divisions in cell elongation zone. Also, ascorbic acid increased production of secondary roots through stimulating cells of pericycle and increasing divisions in this region. Apical meristem of stem treated with ascorbic acid had more convexity homogenous with more chromophilic level. Increasing stem length and number of leaves in plants treated with ascorbic acid could be related to the high cells’ mitotic activity in stem apical meristem. Moreover, ascorbic acid could stimulate cell division, increasing area of meristem zone, and effective on severity of differentiation
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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In vitro proliferation and ex vitro rooting of microshoots of lisianthus using auxin and cytokinin on solid, liquid and double-phase culture systems

63%
Kaviani B., Bahari B.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2019
|
tom 18
|
nr 4
A protocol was developed for high frequency and low cost of in vitro shoot proliferation and ex vitro rooting of Eustoma grandiflorum (Gentianaceae) on solid medium. Shoot tips as explants were cultured on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.00, 0.01, 0.10 and 1.00 mg l–1) and 6-benzylaminopurine (BAP) (0.00, 0.50, 2.00 and 5.00 mg l–1). Three culture media systems (solid, liquid and double-phase) were applied. None of the explants cultured on liquid and double-phase media resulted in live plant production. Maximum axillary shoot number (54.45) was recorded in the plantlets treated with 0.10 mg l–1 2,4-D in combination with 5.00 mg l–1 BAP. Treatment of 0.01 mg l–1 2,4-D along with 0.50 mg l–1 BAP produced maximum node number and internode length. Some shoots produced on medium containing plant growth regulators (PGRs) were rooted in soil. The largest number (5.50/plantlet) and longest length of root (7.75 cm/plantlet) were obtained in ex vitro condition on the base of shoots produced in culture medium enriched with 0.10 mg l–1 2,4-D along with 0.50 mg l–1 BAP. The combination of 1.00 mg l–1 2,4-D and 0.50 mg l–1 BAP was found to be the most suitable PGRs for obtaining the highest callus weight. The most fresh weight was calculated from plantlets grown on the medium containing 0.10 mg l–1 2,4-D along with 5.00 mg l–1 BAP. Maximum dry weight was obtained in free-PGRs medium. About 90% of the rooted plantlets were established successfully in cultivation beds. Acclimatized plants were morphologically similar to the mother plants.
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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The growth of Camellia in peanut shelles compost media in different concentrations of potassium

63%
Torkashvand A. M., Kaviani B.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2014
|
tom 13
|
nr 5
Agricultural wastes management is a step toward sustainable agriculture. Peanut shelles as remained wastes of cultivating peanut has considerable volume which its compost can be used as available sources of ornamental plants medium. A factorial experiment based on randomized completely blocks design was conducted to investigate the impact of peanut shelles compost as the growth medium of Camellia in different concentrations of potassium. Fifteen treatments, every treatment at three replicates and 45 plots were used for experiment. The rooted cuttings of Camellia were planted and periodic watering with Hoagland solution was performed two times per week. After five months, the plants growth indices and the concentration and uptake nutrients were measured. Results showed that peanut shelles compost increased growth indices than in the control. In the most growth indices, the plant indicated a better response to 40% (w/w) compost and finally 60%. The results are indicator of providing the needed potassium for plant by peanut shelles compost than in the control.
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Geranium (Pelargonium graveolens) extract and mechanical treatment improve water relation, enzyme activity and longevity of cut chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) flowers

51%
Hashemabadi D., Abedini-Aboksari H., Sedaghathoor S., Kaviani B.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2016
|
tom 15
|
nr 5
Application of safe organic compounds for the environment is an important approach to increase the longevity of cut flowers. Essences and herbal extracts are compounds having antimicrobial properties, thereby increasing the post-harvest life of cut flowers. Mechanical treatments such as splitting in the stem end also increase longevity of cut flowers. Essences and extracts of geranium (Pelargonium graveolens) are biological compounds with antimicrobial and antioxidant properties that can be used as a vase solution. In this study, different concentrations of rose scented geranium extract and stem end slot were used to improve post-harvest life of cut chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) flowers. The results showed that longevity of cut flowers treated in vase solution containing 100 mg l-1 rose scented geranium extract (18.43 days) with a slot of 5 cm at the stem end was significantly more than that of the control (8.11 days). Water status, microbial population in vase solution and stem end, ionic leakage, and enzymes activities were significantly different between treated cut flowers and control.
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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The role of Pseudomonas strains and arbuscular mycorrhiza fungi as organic phosphate-solubilizing in the yield and quality improvement of strawberry (Fragaria × ananassa Duch., cv. Selva) fruit

51%
Ansari M. H., Hashemabadi D., Mahdavi M., Kaviani B.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2018
|
tom 17
|
nr 4
This study evaluated the effect of Pseudomonas strains and arbuscular mycorrhiza fungi (AMF) in enhancing strawberry yield and phenolic and antioxidant capacity on a phosphorus (P) deficient calcareous soil. The experiments were conducted in three replicates with six treatments (four Pseudomonas strains, AMF and control) and three rates of P-fertilizer (0, 75, 150 kg P ha–1). Application of higher phosphate rates decreased total antioxidant capacity, total phenolic and flavonols content, whereas AMF and Pseudomonas strains increased quality and P concentration of fruit. The use of AMF and Pseudomonas strains resulted in better quality when used along with 75 kg P ha–1. These results demonstrated that the rhizospheric microorganisms improved the quality of fruit, especially when they applied in combination with lower rates of chemical fertilizers. Therefore, application of these microorganisms in sustainable agriculture is recommended.
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

An improved procedure for in vivo and in vitro propagation of Buxus hyrcana, an ornamental shrub critically at risk of extinction

51%
Negahdar N., Hashemabadi D., Kaviani B.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2019
|
tom 100
|
nr 4
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Micropropagation of Orchis catasetum-a rare and endangered orchid

51%
Baker A., Kaviani B., Nematzadeh G., Negahdar N.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2014
|
tom 13
|
nr 2
The application of modern biotechnology for mass propagation of rare and endangered species needs to develop a proper in vitro protocol. Here, a protocol was developed for high frequency in vitro multiplication of an endangered orchid, Orchis catasetum. Protocorms, as explants were cultured on Murashige and Skoog (MS) medium fortified with different concentrations of N6 -benzyladenine (BA), Į-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) either individually or in combination. A combination of 0.5 mg·L BA and 0.5 mg·L NAA was found to be suitable for maximum protocorm-like bodies (PLBs) regeneration (20.40 × plantlet⁻¹). The largest number of root (7.16 × plantlet⁻¹) and leaf (10.10 × plantlet⁻¹), also the highest plant height (114.20 mm × plantlet⁻¹) and root length (193.40 mm × plantlet⁻¹) were obtained on MS medium supplemented with 0.5 mg·L BA a long with 0.5 mg · L NAA. Plantlets with well-developed leaves and roots were transplanted to pots filled with perlite, wood pieces, ionolite and mineral cartridge shell (1:1:1:1), also perlite individually and transferred to the greenhouse. Upon ex vitro transfer, 100% of plants survived.
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Exploring fungal endophytes as biocontrol agents against rice blast disease

51%
Motlagh M. R. S., Kulus D., Kaviani B., Habibollahi H.
Acta Agrobotanica
|
2023
|
tom 76
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Cold storage and cryopreservation by encapsulation-dehydration of Cephalanthera rubra (L.) Rich., a threatened orchid species

51%
Azad M. Z., Kaviani B., Sedaghathoor S.
Acta Agrobotanica
|
2024
|
tom 77
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

In vitro flowering and micropropagation of Lisianthus (Eustoma grandiflorum) in response to plant growth regulators (NAA and BA)

45%
Kaviani B., Zamiraee F., Zanjani S. B., Tarang A., Torkashvand A. M.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2014
|
tom 13
|
nr 4
In vitro flowering and micropropagation are useful for plant breeding programs and commercial production of important ornamental plants. In vitro conditions including media components, kind, concentration and ratio of plant growth regulators and culture conditions significantly affect in vitro flowering and micropropagation. There is no any report dealing with the in vitro flowering of Lisianthus (Eustoma grandiflorum). Here, a protocol was developed for flowering and high frequency in vitro micropropagation of E. grandiflorum, an ornamental plant. Micropropagation is an effective tools for propagation of ornamental plants in large scale. The aim of the present study was to evaluate the effect of different concentrations of NAA and BA on micropropagation and flowering of Lisianthus, in vitro. Used culture medium was MS enriched with 0, 0.1, 0.2 and 2 mg L-1 of NAA and BA. In establishment process of explants, the most shoot length (2.07 cm per plant) was obtained on medium supplemented with 0.1 mg L-1 BA (without NAA). Maximum shoot number (5.80 per plant) was produced in medium containing 0.1 mg L-1 BA along with 0.2 mg L-1 NAA. Bud explants in culture media containing 0.2 mg L-1 NAA (without BA) and 0.1 mg L-1 NAA along with 2 mg L-1 BA produced maximum node number (3.20 per plant). The largest number of root (14.53 per plant) and root length (3.87 cm per plant) were produced on 0.2 mg L-1 NAA without BA, also 0.2 mg L-1 BA plus 0.2 mg L-1 NAA and 0.2 mg L-1 BA without NAA. Explants produced flower on medium containing 0.1 mg L-1 BA along with 0.1 mg L-1 NAA without transition of callus formation. Flower was produced from callus in medium containing 0.1 mg L-1 BA along with 2 mg L-1 NAA. Regenerated plants showed 98% survival in greenhouse during acclimatization. Acclimatized plants were morphologically similar to the mother plants.
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