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Proteus mirabilis isolates (n=177), collected between 1996 and 2000 in four hospitals in the West Pomeranian area of Poland, were characterized by antibiotype and pulsed-field gel electrophoresis (PFGE). The selected isolates were collected from different wards (intensive care unit, surgery, internal medicine, and urology). The strains were cultured from various specimen types, mostly from urine, wound samples, bronchial exudates and sputa. The identification was done by biochemical test ID 32E ATB (bioMerieux). Analysis of PFGE patterns was based on comparison of the banding patterns obtained by PFGE of chromosomal DNA digested with SHI enzyme. Among all P. mirabilis isolates tested three major genotypes A (A1-A7), B (B1-B4), C (C1-C5) and 71 unique patterns were identified. The same genotypes were obtained from different patients, treated in different wards and hospitals during a 5-year period. The strains which belonged to the genotypes A and B were multiresistant and most of them produced ESBL; genotype C was more sensitive to antibiotics.
Przedstawiono wyniki badań, których analiza pozwoliła na zidentyfikowanie dwóch, wieloopornych, epidemicznych szczepów MRSA w Państwowym Szpitalu Klinicznym Nr 2 w Szczecinie.
Recently, interest in essential oils used in natural medicine, has been increasing. Essential oils are still being tested for their potential uses as an alternative remedies for the treatment of many infectious diseases. The aim of the study was to evaluate antibacterial properties of commercial essential oils (rosemary, caraway and fennel) to reduce the number of Staphylococcus aureus and Escherichia coli. The antibacterial activity of essential oils was investigated by agar dilution method. The result of experiments showed that essential oils contained in microbiological media significantly reduced the number of S. aureus and E. coli cells. The best antibacterial properties possessed caraway oil: 1 mg/g for S. aureus and 10 mg/g for E. coli, weaker rosemary (5 mg/g) and fennel (20 mg/g) oils. Results confirmed the inhibiting effect of commercial essential oils on S. aureus and E. coli and provide a scientific ground for future research.
Badano wpływ zespołu flawonoidowo-saponozydowego (FSC) na odpowiedź komórkową (odczyn nadwrażliwości na DNCB), odpowiedź humoralną (poziom przeciwciał dla gronkowców, pałeczek okrężnicy i antygenu Forssmana; występowanie komórek produkujących przeciwciała PFC) oraz aktywność fagocytarną granulocytów u królików, świnek morskich i myszy. FSC podawano w dawce 5 i 10 mg/kg masy ciała przez 7 dni łącznie z antygenem (szczepionka gronkowcowa, krwinki czerwone barana) lub przed immunizacją. Stwierdzono pobudzający wpływ FSC na aktywność fagocytarną granulocytów obojętnochłonnych, odpowiedź humoralną i komórkową.
A total of 90 Pseudomonas aeruginosa strains isolated from 4 hospitals in the west-north region of Poland were studied by arbitrarily primed polymerase chain reaction (AP-PCR). AP-PCR results revealed the presence of 11 main groups of patterns (A-K) and 5 unique patterns among isolates. Generally, they were characterized by high resistance to antibiotics tested and significant differences in serogroups and types of growth on Cetrimide Agar medium. It was observed that clonally related strains were isolated from patients within the same ward, among different wards as well as in distant hospitals.
Vaginal candidiasis is a common problem of clinical practice. Many studies have been conducted to explain its origin but only a few have included Polish women. The aim of the study was to determine the prevalence and similarity of oral, anal and vaginal Candida albicans strains isolated from Polish women with vaginal candidiasis. The study involved 20 from 37 recruited women. Swab samples were collected from their vagina, anus, and oral cavity at two-month intervals. All the women were treated with nystatin. Yeast were recovered and identified by the germ-tube test, API /Vitek system, typed by API ZYM and RAPD-PCR. Chi-square test was used to analyze the data. A total of 170 Candida albicans isolates were recovered from 180 samples collected 3 times from 3 sites of 20 women. Positive yeast vaginal cultures were found in all patients before administration of nystatin. Vaginal yeast recovery rate was decreased statistically significant in both follow-up visits (p =0.001; p =0.003). The same and different genotypes/biotypes were found concomitantly in a few body sites and/ or repeatedly at time interval from the same body site. The results support the concept of dynamic exchange of yeast within one woman and endogenous or exogenous origin of vaginal candidiasis.
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