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Eight important red wine grape varieties (Alicante, Kuntra Karasakiz, İrikara, Gabarnet Franch, Cinsaut, Gamay, Merlot and Syrah) which are use for production for red wine by Doluca winery were analyzed. The highest AA (percentage of inhibition on peroxidation in linoleic acid system) was obtained in Gabarnet Franch and Merlot extracts as 90.25% and 90.15% respectively. The lowest AA was determined in İrikara (83.20%) which had the lowest phenol content. Total phenol content (TP) was varied between 1376 (İrikara) and 2329 µg × ml-1 GAE equivalent in methanol extracts (Merlot). Total anthocyanin content (TA) was ranged from 253.5 mg × kg-1 (İrikara) to 2488.4 mg × kg-1 (Alicante). The lowest PPO activity was found in Merlot (0.070 U × ml-1 × min) and the highest activity in Gamay (1.155 U × ml-1 × min). Total sugar content in the analyzed varieties was changed from 15.30% (Syrah) to 22.64 (Merlot). The examinations showed that AA and TP significant correlation (r2 = 0.854**) but less significantly related with TA (r2 = 0.263).
In this study, the antioxidant activities of Strawberry tree (Arbutus unedo L.) flowers at flowering period and fruits during ripening period were investigated, along with some ripening characteristics such as color, reducing sugar and acidity. Antioxidant activity characteristics were investigated using by the methods of DPPH (1,1-Diphenyl-2- -picryl-hydrazyl) scavenging activity, ȕ-carotene bleaching, reducing power, metal chelating capacity, superoxide anion scavenging and hydrogen peroxide scavenging activity in water, ethanol and methanol extracts. The total phenolic content of flowers in water extracts was found to be 232.38 ± 7.19 mg GAE . g-1 extract, and the DPPH activity was 81.3 ± 0.49% at 50 μg·ml⁻¹ concentration. In the ripening stages, the fully red fruits were determined higher antioxidant capacity than green and yellow fruits, except H₂O₂ scavenging activity which was highest in green fruit. In correlation study, the highest relationship was found between total phenolic content with reducing power (r 2 = 0.987**, P < 0.01), while the lowest with H₂O₂ scavenging activity (r 2 = 0.519*, P < 0.05).
The total phenolic content (TPC), total fl avonoid content (TFC), and the antioxidant activity of soursop (Annona muricata L.) leaf, fruit pulp, seed, and peel extracts obtained using successive extraction with hexane (Hxn), dichloromethane (DCM), ethyl acetate (EtOAc), and methanol (MeOH) were determined. The Hxn soursop seed extract was analysed by GC-MS. The highest TPC was determined in MeOH extracts. MeOH and EtOAc extracts were rich sources of fl avonoids. Generally, soursop leaf and fruit pulp extracts had the highest and the lowest both TPC and TFC, respectively. Fatty acids were dominant in the Hxn seed extract. Among antioxidants, terpenoids ((E)-nerolidolas as dominant) and phytosterols ((3-β)-stigmast-5-en-3-ol with high content) were identifi ed. The soursop seed, followed by leaf and peel extracts (MeOH and EtOAc) had the highest DPPH• scavenging activity, TEAC, FRAP, and CUPRAC. Antioxidant activity of peel extracts (MeOH and EtOAc) was particularly high in β-carotene-linoleic acid emulsion system. Strong correlations were found between TPC, TFC, TEAC, FRAP, and results of DPPH assay. In conclusion, soursop leaves and fruit seeds and peels, which are cheap, waste plant material, could be considered as a source of phenolic antioxidants with a high antioxidant activity.
The antioxidant potential and phenolic compounds content were investigated in ten white bean varieties widely consumed in Turkey. Total phenolic contents of seeds varied between 0.33 and 0.63 mg GAE/g. The Trolox Equivalent Antioxidant Capacity (TEAC) determined by the ABTS assay and the Ferric-Reducing Antioxidant Power (FRAP) of bean varieties ranged from 3.50 to 5.17 μmol Trolox/g seed and from 7.99 to 11.20 μmol Fe2+/g seed, respectively. Strong correlations were found between total phenolic content and FRAP (r=0.850) and between TEAC and FRAP (r=0.734). The antioxidant activity was investigated in a β-carotene-linoleic acid model system, as well. Differences in the inhibition of emulsion oxidation by extracts of white bean varieties were slight. The RP-HPLC fi ngerprint analysis of extracts showed the presence of five dominant phenolic compounds which were described as ferulic or caffeic acids derivatives. Four of them signifi cantly contributed to reducing power and antiradical activity against ABTS•+ of extracts.
In the GC-MS analysis, 81 bioactive phytochemical compounds were identified in hexane extracts of Cirsium creticum and Cirsium italicum. Terpenoids constituted the main fractions of C. italicum (70.95%), while hydrocarbons were most abundant compounds of C. creticum (41.11%). The antibacterial activity and antifungal activity of extracts from two Cirsium species was tested using microdilution methods. According to the results of antibacterial activity, the highest inhibition effect of C. creticum was found on B. subtilis, E. coli and P. aeruginosa. The highest inhibition effect of C. italicum was found on B. subtilis. In the results of antifungal activity, the highest inhibition effect of C. creticum was found on P. chrysogenum and the highest inhibition effect of C. italicum was found on C. krusei. The present work is the first report on hexane extracts compounds of two Cirsium species as well as antibacterial and antifungal activities.
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