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The aim of our study was to examine the efficacy of various disinfectants originally recommended for use in the food industry or medicine, in reducing bacteria Clavibacter michiganensis ssp. sepedonicus. Disinfecting preparations: Pro-Tech, Bakterierent, Bacto Detsan, Mexept, Diesis Forte, Apesin AP 300 were tested taking into account various parameters such as concentration of working solution, environment temperature and duration of treatment. Studies showed, that all tested substances were effective in reducing the growth of bacteria C. michiganensis ssp. sepedonicus in concentrations recommended by the manufacturers (both at 4°C and 20°C), only if duration of procedure was no shorter than 15 minutes. For Mexept, Diesis Forte and Apesin AP 300 shorter disinfection time did not affect the efficacy in reducing the growth of bacteria Cms. Other experiments included the impact of metal surfaces on the effectiveness of selected disinfectants. Bactericidal activity against bacteria C. michiganensis ssp. sepedonicus showed: Bakterierent 2%, Bacto Detsan 1%, Mexept 1%, Apesin AP 300 2%, Pro-Tech 0.5%, Diesis Forte 0.5% (sometimes concentrations active against Cms were higher than those recommended by the manufacturer of the preparation). A prolonged for 15 minutes disinfection procedure was sufficient to obtain a bactericidal effect. As a result of the chosen method of disinfection the type of different treated surfaces such as metal, steel, wood or concrete did not affect significantly the effectiveness of antimicrobial preparations with exception of Mexept and Pro-Tech.
The aim of the performed research was to investigate the occurrence of bacterial diseases of maize in Poland and also a characterization and diagnostics of maize pathogenic bacterial species. Using biological, biochemical, serological and molecular methods a collection of nearly 200 isolates was analyzed. It was found that bacterial isolates belonged to the following genera: Erwinia, Pantoea, Xanthomonas, Xylophilus, Acidovorax, Enterobacter, Pseudomonas i Bacillus. By all used diagnostics methods the presence of 4 isolates of Pantoea ananatis species was confirmed. This species is well known bacterial plant pathogen of maize and many other plants. However, its presence was recorded in Poland for the first time. A bacterial population naturally existing together with plant pathogenic Pantoea ananatis was also characterized.
The aim of the presented studies was to determine a casual agent of zucchini soft rot under the field conditions in the region of Kujawy. In 2009 and 2010, after heavy rain and high temperatures, a mass occurrence of soft rot on the zucchini stems was recorded, leading to a significant reduction in the yield. From zucchini diseased tissue with the soft rot symptoms, some cultures of bacteria were isolated. The isolates giving a positive reaction in pathogenicity test were marked as C1, C2 and C3. Phenotypic characterization of the C1 and C3 isolates allowed to classify them into Erwinia genus and the isolate C2 into Corynebacterium. The further identification using BIOLOG system and sequencing 16S rDNA partial genes, allowed finally to identicate the isolates C1 and C3 as Pectobacterium carotovorum ssp. carotovorum and isolate C2 as Corynebacterium. Studied isolates caused strong soft rot on cucumber and zucchini slices in less than 24 hours after inoculation. The same isolates were reisolated from rotting tissue what confirmed their identity with those ones used for inoculation.
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