The aim of the experiment was an attempt at answering the question if an excessive supply of iron has an influence on the content of GAG in the liver. The experiment was carried out using Chinchilla rabbits (3.2±0.1 kg b.w.). The animals were divided into 3 groups (7 rabbits in each group) and one control group (5 rabbits). The animals in each group except the control group received a pharmaceutical form of iron (intramuscular injection of Jectofer 15 mg/kg b.w./day) for 10, 90 and 120 days, the control group received 0.9% NaCl in an identical manner. Respectively after 10,90 and 120 days the animals were put to sleep (penthobarbital sodium), and after that GAG were isolated from their livers. Glycosaminoglicans were isolated using Svejcer method as modified by Wosicki. The GAG quantity was determined by Bitter and Muiry method by means of glucuronic acid contained in it. After 10 days of Jectofer administration we noticed a 15% decrease of GAG quantity in rabbits’ livers in contrast with the control group; on the other hand after 90 and 120 days of the experiment we noticed increased GAG quantity in the investigated livers by 33% and 62% respectively compared with controls. The obtained results permit us to ascertain that an excessive supply of iron in rats leads to restructuring in the connective tissue of the liver, the noticed changes of GAG quantity were the proof of this One of the consequences may be the hepatic in jury. Although our experiment cannot give a sufficient answer as to how and why excessive accumulation of iron causes the increase of GAG quantity in rabbits’ livers after a long lasting exposition on high doses of Jectofer, with great probability we can say that all those changes are the result of the disturbance of oxidative homeostasis in the liver.
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