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1

Novel processing techniques and their effects on glucosinolates and membrane associated myrosinases in broccoli

100%
Frandsen H. B., Sorensen H., Sorensen J. C.
Polish Journal of Food and Nutrition Sciences
|
2011
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tom 61
|
nr Suppl.1
2

Intact glucosinolates as bioactive food components

100%
Sorensen S., Blok Frandsen H., Sorensen H., Sorensen J. C.
Polish Journal of Food and Nutrition Sciences
|
2011
|
tom 61
|
nr Suppl.1
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Quantitative extraction of oil from plant material with supercritical carbon dioxide

100%
Buskov S., Sorensen H., Sorensen J. C., Sorensen S.
Polish Journal of Food and Nutrition Sciences
|
1997
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tom 06
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nr 3
Supercritical fluid extraction (SFE) both with CO2 and CO2/hexane has been developed as a method of analysis for lipids in cruciferous oilseed, rapeseed products and lupin. The SFE procedure used for quantitative determination of oil was compared to traditional Soxhlet oil extraction. The quantitative values obtained with SFE were equal to those obtained with the Soxhlet procedure with respect to oil and fat contents. The oil obtained by SFE had a much lower phosphorus content (below 14 mg/kg compared to 200-530 mg/kg in oil obtained with Soxhlet). When adding hexane to the supercritical fluid in concentrations varying from 50 to 150 mL/L CO2, the solubility of oil in the fluid could be raised up to 600%, from about 30 mg/g without addition of modifier to nearly 200 mg/g when using continuous addition of 150 mL hexane/ L CO2. The phosphorus content was still lower than in oil from Soxhlet (20-64 mg/kg). Therefore, extraction of oil with SFE opens the possibility for selective removal of oil before separate extraction and determination of amphiphilic compounds, e.g. phospholipids. The carotenoid and chlorophyl content in different oils from SFE and Soxhlet were evaluated by UV-VIS determinations. The presence of other amphiphilic compounds was verified and sinapic acid was found to be a quantitatively dominating phenolic compound.
4
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Effect of dietary fibre fractions on in vitro digestibility of rapeseed napin proteins

88%
Joehnke M. S., Sorensen S., Bjergegaard C., Markedal K. E., Sorensen J. C.
Polish Journal of Food and Nutrition Sciences
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2018
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tom 68
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nr 4
Protein digestibility may be Influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing and recombined in mixed samples. Total dietary fibre (TDF) fractions were isolated from rapeseed hulls (TDF-RH) and purifi ed rapeseed embryo fibres (TDF-RE). The effect of TDF sources on in vitro protein digestibility (IVPD) of a rapeseed protein concentrate rich in napin proteins (RP2) was assessed at three inclusion levels (200, 333, and 500 mg/g DM) using a sequential transient proteolysis by pepsin (1 h) and pancreatin (1 h). The IVPD of RP2 was dose-dependently decreased upon addition of hull fibres at all inclusion levels (8.9-26.6%; P<0.05), whereas the effect of embryo fibres was of a markedly lower magnitude and only signifi cant at the medium to high levels (7.3-8.9%; P<0.05). These results demonstrated that TDF fractions obtained from rapeseed differentially affect the protein digestibility of rapeseed napin proteins depending on the fibre source and inclusion level.
5
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Analysis of phosphorylated aldoses based on group separation and reductive tryptamine derivatisation prior to HPCE revealing initial products of the Maillard reactions

76%
Bjergegaard C., Meinert L., Mortensen K., Moller P., Sorensen H., Sorensen J. C.
Polish Journal of Food and Nutrition Sciences
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2007
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tom 57
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nr 3
345-352
6

Direct MRI-guided stereotaxic viral mediated gene transfer of alpha-synuclein in the Gottingen minipig CNS

64%
Glud A. N., Hedegaard C., Nielsen M. S., Sorensen J. C., Bendixen C., Jensen P. H., Mogensen P. H., Larsen K., Bjarkam C. R.
Acta Neurobiologiae Experimentalis
|
2011
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tom 71
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nr 4
The aim was to establish a non-primate large animal PD model by lentiviral vector mediated mutant alpha-synuclein overexpression in the substantia nigra. Lentivirus encoding A53T alpha-synuclein (6 x 2.5 ^l) was stereotaxically injected into the substantia nigra of six adult female Gottingen minipigs. Contralateral control injections encoding enhanced green fluorescent protein (EGFP) were performed. Gait-analysis was performed pre- and postoperatively. PCR of the transgenes and immunohistochemical staining against alpha-synuclein, EGFP, GFAP and TH was performed after 20 weeks. Gait- analysis revealed a significant increase in step length and height, and a decrease in the double stand phase. PCR verified the mesencephalic presence of transgenes. IHC analysis showed alpha-synuclein expression in nigral neurons, around the injection tract and in related nigrostriatal projections. The alpha-synuclein positive neurons appeared swollen and vacuolated, in contrast to the EGFP-injected control side. To transduct all nigrostriatal cells with few microinjections, wider dissemination of the transgene must be achieved.
7
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Effects of novel processing techniques on glucosinolates and membrane associated myrosinases in broccoli

64%
Blok Frandsen H., Ejdrup Markedal K., Martin-Belloso O., Sanchez-Vega R., Soliva-Fortuny R., Sorensen H., Sorensen S., Sorensen J. C.
Polish Journal of Food and Nutrition Sciences
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2014
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tom 64
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nr 1
High pressure/high temperature (HP/HT) and pulsed electric field (PEF) treatment of food are among the novel processing techniques considered as alternatives to conventional thermal food processing. Introduction of new processing techniques with fast and gentle processing steps may reveal new possibilities for preservation of healthy bioactive compounds in processed food. However, effects on various food components due to autolysis and fast reactions prior to the applied HP/HT or PEF need to be considered as the total contribution of processing steps affects the obtained food quality. The present experiments were performed on broccoli (Brassica oleracea var. Italica) florets, purée and juice. Specific focus was given to effects of HP/HT and PEF processing on the content of glucosinolates and activities of myrosinase isoenzymes (EC.3.2.1.147) in the broccoli preparations. Certain conditions applied in HP/HT processing of broccoli florets were able to maintain a high level of intact glucosinolates. Treatment at 700 MPa and 20°C for 10 min was found to inactivate myrosinase activity, but also pressure treatments at 300 MPa and 20°C were able to maintain a high level of intact glucosinolates present in the untreated broccoli florets. PEF processing of broccoli purée and juice showed that the myrosinase activities resulted in nearly total glucosinolate transformations as result of autolysis during puréeing and juice making prior to the PEF processing. These data demonstrated that insight into potential effects on myrosinase activities from application of PEF processing implies specific focus on the sample steps preceding the PEF processing.
8

Continuous MPTP intoxication in the Gottingen minipig results in chronic parkinsonian deficits

64%
Nielsen M. S., Glud A. N., Moller A., Mogensen P., Bender D., Sorensen J. C., Doudet D., Bjarkam C. R.
Acta Neurobiologiae Experimentalis
|
2016
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tom 76
|
nr 3
Parkinson’s disease (PD) is a common neurodegenerative disorder, resulting from progressive loss of dopaminergic neurons in the substantia nigra pars compacta (SNc). Neuroprotective therapies in PD are still not available, perhaps because animal models do not imitate the chronic and progressive nature of the clinical state of PD. To address this, we performed a feasibility study aimed at establishing a chronic non-primate large animal PD model in Göttingen minipigs based on continuous infusion of the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Twelve female Göttingen minipigs were divided into groups of 2–4 animals and implanted with infusion pumps for continuous intramuscular MPTP delivery of 4–24 mg MPTP/day for 11 weeks. The animals showed parkinsonian symptoms with bradykinesia, rigidity, coordination and chewing difficulties. Symptoms were stable in the 12 and 18 mg MPTP/day groups, whereas the remaining groups showed partial or full behavioral recovery. Digital gait analysis, high performance liquid chromatography (HPLC) measurements and stereological counts of tyrosine hydroxylase-positive (TH+) neurons in the SNc revealed a dose-related decrease in gait velocity, striatal metabolite levels and neuron numbers with increasing doses of MPTP. No neuronal inclusions were observed, but alpha-synuclein staining intensified with increased cumulative MPTP dosages. We conclude that this large-animal model of chronic MPTP administration in Göttingen minipigs shows trends of stable parkinsonian deficits at 18 mg MPTP/day in all modalities examined. This PD model shares many of the characteristics seen in patients and, although preliminary, holds considerable promise for future pre-clinical trials of neuroprotective therapies.
9

Direct gene transfer in the Göttingen minipig CNS using stereotaxic lentiviral microinjections

64%
Glud A. N., Hedegaard C., Nielsen M. S., Sorensen J. C., Bendixen C., Jensen P. H., Larsen K., Bjarkam C. R.
Acta Neurobiologiae Experimentalis
|
2010
|
tom 70
|
nr 3
We aim to induce direct viral mediated gene transfer in the substantia nigra (SN) of the Göttingen minipig using MRI guided stereotaxic injections of lentiviral vectors encoding enhanced green fluorescent protein (EGFP). Nine female Göttingen minipigs were injected unilaterally into the SN with 6×2.5 μl lentivirus capable of transducing cells and mediating expression of recombinant EGFP. The animals were euthanized after four (n=3) or twenty weeks (n=6). Fresh brain tissue from three animals was used for PCR. The remaining six brains were cryo- or paraffin-sectioned for fluorescence, Nissl-, and immunohistochemical EGFP visualization. EGFP was seen in nigral neurons, axons, glial cells, endothelial cells, and in nigral fibers targeting the striatum. PCR-based detection confirmed the presence of the transgene in SN, whereas all other examined brain areas were negative, indicating that the immunohistochemically detected EGFP in the striatum derived from transfected nigral cells.
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