Synthesis of siderophores by soil bacteria of the genus Pseudomonas under various culture conditions

• Autorzy: Jankiewicz U

Warianty tytułu

PL

Synteza sideroforów przez glebowe bakterie z rodzaju Pseudomonas w zmiennych warunkach hodowli

• Języki publikacji: EN

Abstrakty

EN

The ability of six strains belonging to the genus Pseudomonas isolated from the rhizosphere of wheat to produce pyoverdin was examined. The studied strains demonstrated a varied level of production of the siderophore, depending on the culture conditions. The highest level of pyoverdin was determined after 72 hours of growth at 20-25°C in iron-free medium supplemented with succinate. The synthesis of pyoverdin by all the strains studied was strongly repressed by the addition of iron ions (III) to the growth medium. Calcium, cadmium and magnesium ions stimulated the synthesis of the siderophore examined, whereas zinc and lead ions partially decreased its level. Enrichment of the growth medium in cobalt ions completely inhibited the synthesis of siderophores as well as growth of the bacteria.

PL

Zbadano zdolność sześciu szczepów z rodzaju Pseudomonas wyizolowanych z ryzosfery pszenicy do produkcji piowerdyny (pyoverdin). Badane szczepy wykazywały zróżnicowany poziom produkcji tego sideroforu w zależności od warunków hodowli. Wykazano, że syntetyzowały one największą ilość piowerdyny w 72. godzinie hodowli i w temperaturze 20-25°C, na bezżelazowej bursztynianowej pożywce. Synteza piowerdyny u wszystkich badanych szczepów podlegała silnej represji po dodaniu do podłoża hodowlanego jonów żelaza (III). Jony wapnia, kadmu i magnezu w niewielkim stopniu stymulowały syntezę badanego sideroforu, natomiast jony cynku i ołowiu obniżały jej poziom. Wzbogacenie podłoża hodowlanego w jony kobaltu całkowicie zahamowało syntezę sideroforów i wzrost bakterii.

Słowa kluczowe

EN

siderophore synthesis; culture condition; soil bacteria; Pseudomonas; pyoverdin; iron limitation

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Agricultura
• Rocznik: 2006
• Tom: 05
• Numer: 2
• Opis fizyczny: p.33-44,fig.,ref.

Twórcy

autor

Jankiewicz U

• Warsaw Agricultural University, Nowoursynowska 159, 02-776 Warsaw, Poland

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