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2006 | 62 | 12 |

Tytuł artykułu

Ocena reaktywnosci leukocytow krwi obwodowej u szczurow anemizowanych

Warianty tytułu

EN
Evaluation of the reactivity of blood leukocytes in anemized rats

Języki publikacji

PL

Abstrakty

EN
The reactivity of rat blood leukocytes after recurrent blood losses was examined. The blood samples were collected from the heart, three times in seven-day intervals. The volume of each sample was approximately 15% of the total blood volume. The functional changes in leukocytes were determined utilizing a test of radial segmentation of nuclei (RS) in mononuclear leukocytes and a test of Saccharomyces cerevisiae yeast phagocytosis. Our results demonstrate that sequential blood loss induced a decrease in the number of mononuclear cells indicating RS from 21.2% after 1st blood sampling up to 13% and 14% in following samplings: a decrease in number of phagocytic granulocytes from 49.5% after 1st blood sampling up to 41% and 39.3% after 2nd and 3rd sampling, respectively; and an increase in the number of phagocytic mononuclear blood cells from 8.5% after the 1st sampling up to 9.2% and 12.7% after the 2nd and 3rd blood samplings respectively. We affirm that this frequent blood loss modified the reactivity of blood leukocytes but did not change the WBC quantity in blood.

Wydawca

-

Rocznik

Tom

62

Numer

12

Opis fizyczny

s.1435-1438,fot.,rys.,tab.,bibliogr.

Twórcy

  • Akademia Rolnicza, ul.Norwida 31, 50-375 Wroclaw
autor
autor
autor

Bibliografia

  • 1.Barañski S., Czerski P., Krzemiñska-£awkowicz I., Krzymowski T., £awkowicz W.: Układ krwiotwórczy zwierząt laboratoryjnych. PWN, Warszawa 1962, 80.
  • 2.Bershadsky A. A. D., Gelfand V. I.: Role of ATP in the regulation of stability of cytoskeletal structures. Cell Biol. Inter. Rep. 1983, 7, 173-187.
  • 3.Chuadry I. H., Ayala A.: Mechanism of increased susceptibility to infection following hemorrhage. Am. J. Sur. 1993, 165 (2 A suppl.), 59S-67S.
  • 4.Chuanyu Li, Jackson R. M.: Reactive species mechanisms of cellular hypoxia-eoxygenation injury. Am. J. Physiol. Cell Physiol. 20002, 282, C227-C241.
  • 5.Conforti L., Petrovic M., Mohammad D., Lee S., Ma Q., Barone S., Filipovich A. H.: Hypoxia regulates expression and activity of Kv1.3 channels in T lymphocytes: a possible role in T cell proliferation. J. Immun. 2003, 170, 695-702.
  • 6.Dong Z., Wang J.: Hypoxia selection of death-resistant cells. J. Biol. Chem. 2004, 279, 9215-9221.
  • 7.Facco M., Zilli Ch., Siviero M., Ermolao A., Travain G., Baesso I., Bonamico S., Cabrelle A., Zaccaria M., Agostini C.: Modulation of immune response by the acute and chronic exposure to high altitude. Med. Sci. Sports Exercise. 2005, 37, 768.
  • 8.Feldman B. F., Zinkl J. G., Jain N. C.: Schalm’s veterinary hematology. Lippincott Williams and Wilkins, Philadelphia 2000.
  • 9.Gardner L. B., Qing Li, Park M. S., Flanagan W. M., Semenza G. L., Chi V. Dang: Hypoxia inhibits G1 /S transition through regulation of p27 expression. J. Biol. Chem. 2001, 276, 7919-7926.
  • 10.Graczyk S., Madej J. A., Pliszczak-Król A., Nowak M., Janaczyk B.: Leukocytes function in the course of tumor progression in a rat model. Acta Sci. Pol., Med. Vet. 2004, 3, 45-51.
  • 11.Lewis J. S., Lee J. A., Underwood J. C. E., Harris A. L., Lewis C. E.: Macrophage responses to hypoxia: relevance to disease mechanisms. J. Leuk. Biol. 1999, 66, 889-900.
  • 12.Min Ding, Robinson J. M., Behrens B. C., Vandre D. D.: The microtubule cytoskeleton in human phagocytic leukocytes is a highly dynamic structure. Eur. J. Cell Biol. 1995, 66, 234-245.
  • 13.Park J. H., Okayama N., Gute D., Krasmanovic A., Battarbee H., Alexander J. S.: Hypoxia/aglycemia increases endothelial permeability: role of second messengers and cytoskeleton. Am. J. Physiol. 1999, 277, C1066-C1074.
  • 14.Pliszczak-Król A.: Wpływ ACTH na RS jąder i aktywność fosfatazy kwaśnej w aktywowanych antygenem limfocytach krwi ptaków. Medycyna Wet. 2001, 57, 676-679.
  • 15.Pliszczak-Król A.: Rola centralnych i obwodowych narządów limfatycznych w powstawaniu radialnej segmentacji (RS) jąder oraz kształtowaniu aktywności fosfatazy kwaśnej w limfocytach krwi kurcząt immunizowanych. Acta Sci. Pol., Med. Vet. 2002, 1, 81-101.
  • 16.SaiRam M., Sharma S. K., Dipti P., Pauline T., Kain A. K., Mongia S. S., Bansal A., Patra B. D., Ilavazhagan G., Devendra K., Selvamurthy W.: Effect of hypobaric hypoxia on immune function in albino rats. Int. J. Biometeorol. 1998, 42, 55-59.
  • 17.San-Oh Yoon, Sejeong Shin, Mercurio A. M.: Hypoxia stimulates carcinoma invasion by stabilizing microtubules and promoting the Rab11 trafficking of the a6b4 integrin. Cancer Res. 2005, 65, 2761-2769.
  • 18.Sanidas D., Garnham A., Mian R.: Activation of human leukocytes by acute hypoxia. Exp. Physiol. 2000, 85, 263-266.
  • 19.Scannell G.: Leukocytes responses to hypoxic/ischemic conditions. New Horiz. 1996, 4, 179-183.
  • 20.Slapnièkova M., Berger J.: Rat neutophil phagocytosis following feed restriction. Comp. Clin. Path. 2002, 11, 172-177.
  • 21.Söderström U.-B., Norberg B., Brandt L.: The oxalate-induced radial segmentation of the nuclei in peripheral blood lymphocytes of different size. Scan. J. Haematol. 1976, 17, 57-61.
  • 22.Suiko Ito: Study on the in vitro Rieder cell. Scand. J. Haematol. 1974, 12, 355-365.
  • 23.Wood J. G., Johnson J. S., Mattioli L. F., Gonzalez N. C.: Systemic hypoxia increases leukocyte emigration and vascular permeability in conscious rats. J. Appl. Physiol. 2000, 89, 1561-1568.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-fb86074c-48e4-431c-bb99-65baf3483909
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