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The aim of the work was to determine monensin, narasin hepatotoxicity and the nature of cell death. Rat hepatocyte model cell line (FAO) was used to investigate two ionophore antibiotic cytotoxic effects estimated by MTT, NRU and KB tests approved by INVITTOX. Additionally, the apoptotic/necrotic nature of cell death was determined by propiodine iodide and HO 342 staining of the cultured hapatocytes. IC₅₀ indices for monensin and narasine estimated by using the MTT test during a 24 hour incubation period were at a level of 0,027 ± 0,001 µM and 0,037 ± 0,001 µM, respectively. However, an incubation period of 48 hrs yielded an equal value - 0,02 µM - for both ionophores. Contrary to the MTT test, NRU and KB estimations demonstrated lower IC₅₀ values for narasine than for monensin. These results correlated to in-vivo acute toxicity and LD₅₀ indices in rats (data from references). The apoptotic nature of hepatocyte death dominated in the cultures. The article also discussed the mechanisms of ionophore induced cytotoxicity.
The purpose of the study was to determine the effect β₂-adrenergic receptor agonist-clenbuterol on tibiae mineralization of female rats with established osteopenia. The experiments were conducted on 30 female 3-months-of-age Wistar rats with an initial body weight of about 250 g. The sham-operation - SHO (n=10) and bilateral ovariectomy - OVX (n=20) were performed. After 60 days of osteopenia induction the ovariectomized rats were divided into a group fed a standard diet (n=10) and a group that received a diet supplied with clenbuterol at a dose of 5 mg/kg. After 14 days of the experiment the tibiae was isolated and tested using a DEXA densitometer, peripheral quantitative computer tomography (pQCT) and three-point bending test. The obtained results proved that a 14 day period of clenbuterol treatment significantly increased mechanical properties content and mineral density, both planar (BMD) and volumetric (vTotBMD) of the tibiae of ovariectomized rats.
Wykonano badania zawartości magnezu w zębach szczura po zastosowaniu dużych dawek hydrokortyzonu w doświadczalnej osteoporozie polekowej. Poziom magnezu w zębach oznaczony za pomocą absorpcyjnej spektrometrii atomowej, zależał od czasu trwania doświadczenia i rodzaju podawanego leku. Po sterydoterapii zaobserwowano hypomagnezemię w zębach szczura.
The aim of the study was to determine whether high doses of Vitamin C may alleviate the effects of oxidative stress caused by ozone, or whether they actually intensify its course and demonstrate pro-oxidative activity. The experiment was conducted on adult male rats divided into 6 groups: Group I - control animals; Group II and III - animals which were simultaneously ozonated and administered intramuscular injections of Vit C in doses of 20 and 40 mg/rat respectively; Group IV and V - non-ozonated animals, which were administered the above doses of Vit C every fifth day for a period of 35 days; Group VI - rats ozonated without Vit C. The results of the study indicated that the highest level of indexes defining the intensity of oxidative stress occurred both in animals which were ozonated and given high doses of Vit C as well as in non-ozonated animals which were given high doses the vitamin. AST activity and glucose levels were also highest in the above groups and displayed a similar pattern. It may thus be assumed that high doses of Vit C do not prevent organisms from ozone-induced oxidative stress, and may even increase its intensity
The experiment was carried out using 32 Wistar strain male rats of 280-320 g body weight. The animals were randomly divided into 2 groups. Control group I were healthy animals (8 rats). In group II, acute pancreatitis was induced by injecting 5% sodium taurocholate solution into the common bile-pancreas duct. Twenty-four hours before the operation, animals were food deprived and were given Dormicum (from a vial) in ad libitum drinking water. After acute pancreatitis induction, the animals of group II were additionally divided into 3 sub-groups: A, B, and C according to the time the disease had developed. Blood and internal organs from sub-group A were sampled after 3 hours, from group B after 24 hours, and from group C - after 48 hours. Organs were homogenized in 20 mM Tris buffer at pH 7.4. Products of lipid peroxidation were determined in supernatant: conjugated dienes, hydroperoxilipid dienes and malonic aldehyde. Total anti-oxidation potential was determined in blood serum along with its components: uric acid, urea and bilirubin. a-amylase in serum activity was also recorded to confirm the development of acute pancreatitis. The results of the study revealed very variable dynamics of lipid peroxidation in organs essential for survival during acute pancreatitis. This suggests that the mechanisms of anti-oxidation defense are not able to successfully protect the organism against oxidation stress during acute pancreatitis, which consequently enhances the risk of multi-organ failure syndrome. A detailed explanation of the oxidation stress role in the course of acute pancreatitis and its complications requires further integrated studies at the molecular level, cellular culturing and on animal models.
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