Rapid detection and quantification of foot-and-mouth disease virus by a real-time reverse transcription PCR

• Autorzy: Niedbalski W , Kesy A.
• Języki publikacji: EN

Abstrakty

EN

A real-time reverse transcription-PCR (rRT-PCR) for the detection of the foot-and-mouth disease virus (FM DV) in samples of archival isolates of FMDV serotypes O, A, C, SAT1-3, and Asia 1 was described. A primer set that targets the IRES region of the FMDV genome and TaqMan probe specific for a highly-conserved region in FMDV RNA IRES region was used. The assay detected the viral RNA in all tested archival serotypes of FMDV. Swine vesicular disease virus (SVDV) and bluetongue virus (BTV) RNA as well as RNA isolated from epithelium taken from healthy uninfected calf (negative control) were undetectable in the test. The detection limit of rRT-PCR was quantified for 1 TCID₅₀. The test can provide quantitative as well as qualitative information and is more sensitive and much faster to perform than the conventional RT-PCR. It can be used in large-scale screening because of its ability to simultaneous analysis up to 96 samples per run. The applied rRT-PCR is accurate, specific, and reliable technique for the detection of FMDV in biological samples. Therefore it is seen as a valuable tool to complement the routine diagnostic procedure for FMD virus diagnosis.

Słowa kluczowe

EN

detection; quantification; diagnosis; real-time reverse transcription polymerase chain reaction; foot-and-mouth disease virus; animal disease; wild animal; domestic animal

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 1
• Opis fizyczny: p.3-7,fig.,ref.

Twórcy

autor

Niedbalski W

• National Veterinary Research Institute, 98-220 Zdunska Wola, Poland

autor

Kesy A.

Bibliografia

• 1. Alexandersen S., Zhang Z., Donaldson A.L., Garland A.J.: Pathogenesis and diagnosis of foot-and-mouth disease. J Comp Pathol 2003, 129, 1-36.
• 2. Callahan J.D., Brown F., Csorio F.A., Sur J.H., Kramer E., Long G.W., Lubroth J., Ellis S.J., Shoulars K.S., Gaffney K.L., Rock D.L., Nelson W.M.: Use of a portable real-time reverse transcriptase-polymerase chain reaction assay for rapid detection of foot-and-mouth disease virus. J Am Vet Med Assoc 2002, 220, 1636- 1642.
• 3. Fernandez J., Aguero M., Romero L., Sanchez C., Belak S., Arias M., Sanchez-Vizcaino J.M.: Rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by the new multiplex RT-PCR. J Virol Methods 2008, 147, 301-311.
• 4. Grubman M.J., Baxt B.: Foot-and-mouth disease. Clin Microbiol Rev 2004, 17, 465-493.
• 5. Hindson B.J., Reid S.M., Baker B.R., Ebert K., Ferris N.P., Tammero L.F., Lenhoff J., Naraghi-Arani P., Vitalis E.A., Slezak T.R., Hullinger P.J., King D.P.: Diagnostic evaluation of multiplex reverse transcription- PCR microsphere array assay for detection of foot-and- mouth and look-alike disease viruses. J Clin Microbiol 2008, 46, 1081-1089.
• 6. http://www.oie.int
• 7. King D.P., Ferris N.P., Shaw A.E., Reid S.M., Hutchings G.H., Giuffre A.C., Robida J.M., Callahan J.D., Nelson W.M., Beckham T.R.: Detection of foot-and-mouth disease virus: comparative diagnostic sensitivity of two independent real-time reverse transcription-polymerase chain reaction assays. J Vet Diagn Invest 2006, 18, 93- 97.
• 8. Lanciotti R.S., Kerst A.J.: Nucleic acid sequence-based amplification assays for rapid detection of West Nile and St. Louis encephalitis viruses. J Clin Microbiol 2001, 39, 4506-4515.
• 9. Niedbalski W.: Application of different diagnostic methods for the detection of SVDV infection in pigs. Bull Vet Inst Pulawy 1999, 43, 11-18.
• 10. O.I.E.: Foot-and-mouth disease. Chapter 2.1.5. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Paris, 2008, pp. 1-37.
• 11. Oleksiewicz M.B., Donaldson A.I., Alexandersen S.: Development of a novel real-time, RT-PCR assay for quantitation of foot-and-mouth disease virus in diverse porcine tissues. J Virol Methods 2001, 92, 23-35.
• 12. Rasmussen T.B., Uttenthal A., de Stricker K, Belak S., Storgaard T.: Development of a novel quantitative realtime R-PCR assay for the simultaneous detection of all serotypes of foot-and-mouth disease virus. Arch Virol 2003,148, 2005-2011.
• 13. Reid S.M., Ferris N.P., Hutchings G.H., Samuel A.R., Knowles N.J.: Primary diagnosis of foot-and-mouth disease by reverse transcription polymerase chain reaction. J Virol Methods 2000, 89, 167-176.
• 14. Reid S.M., Ferris N.P., Hutchings G.H., Zhang Z.D., Belsham G.J., Alexandersen S.: Detection of all seven serotypes of foot-and-mouth disease virus by real-time, fluorogenic reverse transcription polymerase chain reaction assay. J Virol Methods 2002, 105, 67-80.
• 15. Rodriquez A., Martinez-Salas E., Dopazo J., Davila M., Saiz J.C., Sobrino F.: Primer design for specific diagnosis by PCR of highly variable RNA viruses: typing of foot-and-mouth disease virus. Virology 1992, 189, 363-367.
• 16. Rueckert R.R.: Picornaviridae and their replication. In: Fundamental virology, edited by B.N. Field and D.M. Knipe, Raven Press, New York, 1990, pp.507-548.
• 17. Saiz M., De La Morena D.B., Blanco B., Nunez J.I., Fernandez R., Sanchez-Vizcaino J.M.: Detection of foot- and-mouth disease virus from culture and clinical samples by reverse transcription-PCR coupled to restriction enzyme and sequence analysis. Vet Res 2003, 34, 105-117.
• 18. Shaw A.E., Reid S.M., King D.P., Hutchings G.H., Ferris N.P.: Enhanced laboratory diagnosis of foot-and-mouth disease by real-time polymerase chain reaction. Rev Sci Tech Off Int Epiz 2004, 23, 1003-1009.
• 19. Shaw A.E., Reid S.M., Ebert K., Hutchings G.H., Ferris N.P., King D.P.: Implementation of a one-step real -time RT-PCR protocol for diagnosis of foot-and-mouth disease. J Virol Methods 2007, 143, 81-85.
• 20. Vangrysperre W., De Clercq K.: Rapid and sensitive polymerase chain reaction based detection and typing of foot-and-mouth disease virus in clinical samples and cell culture isolates, combined with a simultaneous differentiation with other genomically and/or symptomatically related viruses. Arch Virol 1996, 141, 331-344.