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1

Simplification of a haemolytic micromethod for toxic saponin quantification in alfalfa

100%

Gorski P. M., Jurzysta M.

Acta Agrobotanica

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1988

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tom 41

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nr 2

A simplification of a haemolytic micromethod is presented. In the orginal method, alfalfa (Medicago media Pers.) leaf sap is spotted on the plate covered with a blood-gelatine suspension. In the presented method, the mashed alfalfa pulp is used instead of sap. Due to saponin diffusion and the reaction with erythrocytes, a haemolytic ring appears, which has a width proportional to the concentration of toxic saponins. It is shown that the width of haemolytic ring does not depend on the sample weight ranging from 20 to 100 mg. This allows for the omission of laborious sap pressing and sample quantification. Individual alfalfa plants with different saponin contents were tested using leaf sap and leaf pulp for analyses. Good agreement was found with sap and leaf pulp methods. The correlation obtained by both methods was high, r = 0.87. The modified method requires only a small amount of plant material and makes the analyses of large numbers of individual plants per day possible. The method is especially recommended for breeding purposes.

2

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Quantification of the ash content from biofuel - wood according to ISO 1171 (2003) and EN 14775 (2010)

86%

Dzurenda L., Pnakovic L.

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

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2014

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tom 86

3

Influence of temperature and equilibration time on the quantification of aroma impact compounds in coffee brews

86%

Sanchez-Ayaso L., Ludwig I. A., Paz De Pena M., Cid C.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

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nr Suppl.1

4

Quantification of the potencies of EDRF-releases from isolated rabbit aortic strips

86%

Trybulec M., Dudek R., Radziszewski W., Swierkosz T., Zembowicz A.

Acta Physiologica Polonica

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1990

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tom 41

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nr 1-3

Trybulec M., Dudek R., Radziszewski W., Świerkosz T, Zembowicz A.: Quantification of the potencies of EDRF-releasers from isolated rabbit aortic strips. Acta Physiol. Pol. 1990, 41(1-3): 78-86. We have compared several known releasers of endothelium-derived relaxing factor (EDRF)(13) in respect to their potencies to generate EDRF by endothelium of rabbit aortic strips (RbA) superfused with Krebs’ buffer. The vasorelaxation by EDRF which is equivalent to 10 pmoles of GTN was evoked by 0.7 pmoles of substance P(SP), 50 pmoles of acetylcholine (Ach), 521 pmoles of calcium ionophore A 23187, 2720 pmoles of ADP. Threshold potencies of these agonists are inversely proportional to the maximum amount of EDRF released. Phospholipase C (PLC) from Clostridium perfringens at a dose of 0.1 U caused the relaxation of a similar magnitude. Phospholipase A2 (1 U), thrombin (1 U), bradykinin (30 nmoles) and serotonin (10 pmoles) did not release EDRF. It is concluded that endothelial cells of RbA differ from endothelial cells of other species in their susceptibility to release EDRF in response to various agonists.

5

Quantification of bovine mammary stem/progenitor cells by laser scanning and flow cytometry

86%

Osinska E., Gajewska M., Majewska A., Motyl T.

Animal Science Papers and Reports

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2015

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tom 33

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nr 1

Due to the presence and activity of mammary stem cells (MaSC), growth and remodelling of mammary gland during puberty and lactation cycles is possible. In this study the number of putative mammary stem/progenitor cells was examined in 20 months old non-pregnant Holstein-Friesian heifers. Cells were double-stained with fluorescent dye-conjugated antibodies against stem cell antigen-1 (Sca-1) and fibronectin type III domain containing 3B (FNDC3B), and were analysed Rusing scanning cytometry and flow cytometry. Nuclei were counterstained with Hoechst 33342. Scanning and flow cytometry revealed 2.43±0.32% and 1±0.37% of MaSC in total cell number, respectively.Sca-1posFNDC3Bpos cells did not express estrogen receptor (ERα), confirming their undifferentiated phenotype. In conclusion, scanning cytometry is a preferable method for evaluation of the number and localization of MaSC in situ, whereas, flow cytometry with cell sorting enables further genomic and biochemical analyses of isolated cells.

6

Quantification of phenolic compounds in smoked meat products using different glow smoke conditions

86%

Pohlmann M., Schwagele F., Speer K., Jira W.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

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nr Suppl.1

7

Improved HPLC method for total plasma homocysteine detection and quantification

86%

Sawula W., Banecka-Majkutewicz Z., Kadzinski L., Jakobkiewicz-Banecka J., Wegrzyn G., Nyka W., Banecki B.

Acta Biochimica Polonica

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2008

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tom 55

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nr 1

Recent clinical research has pointed at hyperhomocysteinemia as an independent risk factor in a number of cardiovascular and neurological diseases. We have improved a chromatographic method of total plasma homocysteine measurements in order to obtain higher sensitivity, reliability and reproducibility. The method demonstrates excellent linearity (R = 0.999), range (< 2-100 µM), precision (instrumental RSD 0.06 and method RSD 1.17), accuracy (recovery of 99.92 and RSD 1.27), reproducibility, quantification limit and ruggedness (e.g. pH from 2.0 to 2.5). Because even a small increase in homocysteine level can be a significant risk factor of cardiovascular diseases, such a precise method is required. The constructed method allows the measurement of plasma pyridoxal phosphate, PLP, the co-enzyme form of vitamin B6, on the same column and similar reagents. The developed method has been successfully applied to measure both total plasma and serum homocysteine in a group of acute stroke patients.

8

Challenges and solutions in reference material development for quantification of food allergens

72%

Torok K., Bugyi Z., Hajas L., Adonyi Z., Tomoskozi S.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

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nr Suppl.1

9

Equine herpesvirus type 1 quantification in different types of samples by a real-time PCR

72%

Dzieciatkowski T., Przybylski M., Cymerys J., Turowska A., Chmielewska A., Tucholska A., Banbura M. W.

Polish Journal of Veterinary Sciences

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2009

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tom 12

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nr 3

Equine herpesvirus type 1 (EHV-1) is one of the major viral agents causing diseases in horses common worldwide. A variety of techniques, including PCR, have been used to diagnose EHV-1 infections. In this paper, an attempt of real-time PCR has been described, which uses specific fluorochrome-labeled TaqMan probes for detection of viral DNA. This method does not require post-amplification manipulations, thereby reducing the risk of cross-contamination. The assay was sensitive enough to detect EHV-1 sequences in different clinical samples, as well in mice neuronal cell cultures. The technique was also very specific – there was no cross reaction with other human and equine herpesviruses. Compared to previously used nested PCR technique, the test was more sensitive and should be useful for the common diagnosis based on its specificity and rapidity.

10

Determination of metallothionein in biological fluids using enzyme-linked immunoassay with commercial antibody

72%

Milnerowicz H., Bizon A.

Acta Biochimica Polonica

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2010

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tom 57

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nr 1

99-104

Metallothionein (MT) is a low molecular weight cysteine-rich protein with a number of roles in the pro/antioxidant balance and homeostasis of essential metals, such as zinc and copper, and in the detoxification of heavy metals, such as cadmium and mercury. Until now, detection of metallothionein in biological fluids remained difficult because of a lack of a broadly reactive commercial test. Meaningful comparison of the values of metallothionein concentrations reported by different authors using their specific isolation procedures and different conditions of enzyme-linked immunoassay is difficult due to the absence of a reference material for metallothionein. Therefore in the present study, we describe a quantitative assay for metallothionein in biological fluids such as plasma and urine performed by a direct enzyme-linked immunoassay using a commercially; available monoclonal mouse anti-metallothionein clone E9 antibody and commercial standards of metallothionein from rabbit liver and a custom preparation of metallothionein from human liver. The sensitivity of the assay for the standard containing two isoforms MT-I and MT-II from human liver was 140 pg/well. The reactivity of the commercial standards and standards containing two isoforms MT-I and MT-II isolated from human liver in our laboratory with a commercial monoclonal mouse anti-metallothionein clone E9 antibody were similar. This suggests that the described ELISA test can be useful for determination of metallothionein concentration in biological fluids. The concentrations of metallothionein in human plasma, erythrocyte lysate and in urine of smoking and non-smoking healthy volunteers are reported. Tobacco smoking increases the extracellular metallothionein concentration (plasma and urine) but does not affect the intracellular concentration (erythrocyte lysate).

11

Restoration of a river landscape: Biotopes as a basis for quantification of species diversity and evaluation of landscape quality

72%

Mekotova J., Sarapatka B., Sterba O., Harper D.

International Journal of Ecohydrology and Hydrobiology

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2006

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tom 06

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nr 1-4

43-51

12

Identification and quantification of tylosin in animal feed by liquid chromatography combined with electrospray ionisation mass spectrometry

72%

Przenioslo-Siwczynska M., Grelik A., Kwiatek K.

Journal of Veterinary Research

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2020

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tom 64

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nr 2

13

Application of fast liquid chromatography for antioxidants analysis

72%

Drozdzynska A., Dziedzic K., Kosmider A., Leja K., Czaczyk K., Gorecka D.

Acta Scientiarum Polonorum. Technologia Alimentaria

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2012

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tom 11

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nr 1

Background. An intensive development of the Fast Liquid Chromatography (FLC) has been recently observed. It makes possible to reduce time analysis and improve resolution as well as sensitivity. The aim of this study was to separate the chosen antioxidants optimization using the FLC method. Material and methods. The three various procedures for antioxidants analysis were compared. Mobile phases containing aqueous solution of formie acid, acetic acid, acetonitrile, and methanol were tested. Limit of detection (LOD), limit of quantification (LOQ), linearity and repeatability of each procedures were determined. Results. Developed procedure enabled to separate all analytes and allowed to get low LOD levels and good repeatability. This procedure was used for antioxidants analysis in buckwheat and buckwheat products. Conclusion. Fast Liquid Chromatography allows to reduce time analysis and obtain good validation parameters.

14

Quantification of ecological preferences of the Capercaillie Tetrao urogallus by means of the habitat suitability index: a case study in the Augustow Forest (N-E Poland)

72%

Brzeziecki B., Drozdowski S., Zawadzka D., Zawadzki J.

Polish Journal of Ecology

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2012

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tom 60

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nr 4

Habitat structure and selection by the Capercaillie Tetrao urogallus were assessed in the Augustow Forest (NE Poland), where a population of 50–80 birds and 11 active leks existed. Habitat preferences in the local scale were studied, based on measurements of total 1952 circular sample plots with radius of 15 m. On plots, in total, 10 variables describing forest structure (e.g. successional stage, canopy cover, vertical stand structure, share of Scots pine Pinus sylvestris, shrub layer cover, bilberry Vaccinium myrtillus cover, average height of ground vegetation, occurrence of feeding and roosting trees), as well as signs of Capercaillie presence, were assessed. Then, the model of Habitat Suitability Index (his) was constructed. Plots with high HSI scores were used by Capercaillie more often than expected in a case of a random choice, and those with low scores – less than expected. Capercaillie in the Augustow Forest prefers relatively old, one-layered stands, dominated by Scot pine with a sparsely developed shrub layer. The most important differences between abandoned and active leks were related to shrub cover in ground vegetation and height of ground vegetation and share of bilberry.

15

Quantification of long term wood production according to population explosion during 20th century

72%

Parobek J.

Intercathedra

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2008

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tom 24

16

Rapid detection and quantification of foot-and-mouth disease virus by a real-time reverse transcription PCR

72%

Niedbalski W., Kesy A.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

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nr 1

3-7

A real-time reverse transcription-PCR (rRT-PCR) for the detection of the foot-and-mouth disease virus (FM DV) in samples of archival isolates of FMDV serotypes O, A, C, SAT1-3, and Asia 1 was described. A primer set that targets the IRES region of the FMDV genome and TaqMan probe specific for a highly-conserved region in FMDV RNA IRES region was used. The assay detected the viral RNA in all tested archival serotypes of FMDV. Swine vesicular disease virus (SVDV) and bluetongue virus (BTV) RNA as well as RNA isolated from epithelium taken from healthy uninfected calf (negative control) were undetectable in the test. The detection limit of rRT-PCR was quantified for 1 TCID₅₀. The test can provide quantitative as well as qualitative information and is more sensitive and much faster to perform than the conventional RT-PCR. It can be used in large-scale screening because of its ability to simultaneous analysis up to 96 samples per run. The applied rRT-PCR is accurate, specific, and reliable technique for the detection of FMDV in biological samples. Therefore it is seen as a valuable tool to complement the routine diagnostic procedure for FMD virus diagnosis.

17

Quantification of thioredoxin mRNA expression in the rat hippocampus by real-time PCR following oxidative stress

72%

Yalcin A.

Acta Biochimica Polonica

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2004

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tom 51

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nr 4

Thioredoxin (Trx) is a multifunctional protein with a redox-active disulfide/dithiol in the active site. Thioredoxin, with its redox-regulating and reactive oxygen species (ROS) scavenging activities, plays several important biologic roles both in intracellular and extracellular compartments. The purpose of this report was to quantify the relative expression of Trx in rat hippocampus following an oxidative stress-involving treatment such as kainic acid (KA) using real-time PCR and the 2-AACt method. The relative changes in expression of Trx mRNA in KA-treated and control animals were significantly different as 2.02 ± 0.77 and 1.0 ± 0.26, respectively (P < 0.05). Minimum and maximum n-fold changes in Trx expression in KA-treated and control animals were determined as (1.4-5.2) and (0.8-1.3), respectively. Thus, real-time PCR and the 2-AACt method for data analysis from real-time PCR were found to be an accurate and sensitive method for quantifying Trx mRNA levels.

18

Use of in vitro methods for detection and quantification of antirabies antibodies

72%

Zavadova J., Svrcek S., Madar M., Durove A., Benisek Z., Suliova J.

Bulletin of the Veterinary Institute in Puławy

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1998

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tom 42

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nr 1

19

Quantification of P21 mRNA in biopsy specimens of colon tissue at different stages of neoplastic transformation

72%

Dzierzewicz Z., Kwapisz I., Orchel A., Hudyka K., Wilczok T.

Folia Histochemica et Cytobiologica. Supplement

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2001

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tom 39

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nr 1

20

Quantification of S ambient concentrations by sorption-cumulative method of the Slovac Republic in the Region of Upper Nitra

72%

Lazor P., Tomas J., Vollmannova A., Toth T.

Biuletyn Magnezologiczny

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2001

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tom 06

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nr 4

Emission of sulphur compounds was absorbed from the atmosphere to an alkaline surface of a filter containing sodium carbonate in the region of Nitra - Malanta. Sulphates were determined by titration with ethanolic solution of Ba(C104)2. Results from January 1st - December 31st 1999 show that mean S02 concentrations at Nitra were 18.3 ug.m-3 S02 and 15.4 mg ■ m-2- d-1 SO2. The average (January 1st - December 31st 2000) value of area fallout was 11.6 mg m-2 d -1 S02 and the value of content concentration was 14.9 ug m-3 S02. The area fallout of emission of S is connected with the given meteorological situation (precipitation, average temperature, direction and speed of wind). The fact was confirmed by the results of our experiment.

Ograniczanie wyników

Simplification of a haemolytic micromethod for toxic saponin quantification in alfalfa

Quantification of the ash content from biofuel - wood according to ISO 1171 (2003) and EN 14775 (2010)

Influence of temperature and equilibration time on the quantification of aroma impact compounds in coffee brews

Quantification of the potencies of EDRF-releases from isolated rabbit aortic strips

Quantification of bovine mammary stem/progenitor cells by laser scanning and flow cytometry

Quantification of phenolic compounds in smoked meat products using different glow smoke conditions

Improved HPLC method for total plasma homocysteine detection and quantification

Challenges and solutions in reference material development for quantification of food allergens

Equine herpesvirus type 1 quantification in different types of samples by a real-time PCR

Determination of metallothionein in biological fluids using enzyme-linked immunoassay with commercial antibody

Restoration of a river landscape: Biotopes as a basis for quantification of species diversity and evaluation of landscape quality

Identification and quantification of tylosin in animal feed by liquid chromatography combined with electrospray ionisation mass spectrometry

Application of fast liquid chromatography for antioxidants analysis

Quantification of ecological preferences of the Capercaillie Tetrao urogallus by means of the habitat suitability index: a case study in the Augustow Forest (N-E Poland)

Quantification of long term wood production according to population explosion during 20th century

Rapid detection and quantification of foot-and-mouth disease virus by a real-time reverse transcription PCR