Serum samples collected from 220 cows, 226 pigs, 10 sheep, 8 cats, 62 turkeys, 54 geese, 22 hens, and 7 ducks in Lublin region were tested by passive haemagglutination test with Yersinia enterocolitica (five serotypes) and Yersinia pseudotuberculosis (two serotypes) antigens. Y. enterocolitica antibodies were found in 23.2%, 17.2%, 11.3%, and 31.8% of cows, pigs, turkeys, and hens, respectively, while Y. pseudotuberculosis antibodies in 18.2%, 3.6%, 32.2%, and 13.6%, respectively. No positive response occurred in sheep, cats, geese, and ducks. The antibody response of cows to Y. enterocolitica characterised itself by high frequency of reactions to virulent serotype O:8 (17.3%), which was greater in comparison to that with the remaining four serotypes (5.9%). The results suggest the importance of the consumption of undercooked beef and contaminated milk in epidemiology of yersiniosis.