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1

The analysis of the plant genome of the population of Betula nana in the Linie Reserve using RAPD-PCR method

100%
Burnicka O., Marszal J., Dabrowska G.
Polish Journal of Natural Sciences. Supplement
|
2003
|
tom 01
2

The model plant Arabidopsis thaliana: Implications from analysis of gene redundance to genome functional plasticity

88%
Sadowski J., Babula D., Kaczmarek M., Ziolkowski P., Stanko A.
Polish Journal of Natural Sciences. Supplement
|
2003
|
tom 01
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Exploring computational protein fishing (CPF) to identify argonaute proteins from sequenced crop genomes

75%
Basu P., Ganguli S., Gupta S., Datta A.
International Letters of Natural Sciences
|
2015
|
tom 06
Plant RNA interference has been a very well studied phenomenon since its discovery. We are well versed with the types of small noncoding RNAs that are prevalent in the plant systems and their pathways of biogenesis and subsequent actions. However, apart from model plant systems such as Arabidopsis and Oryza, very little information is available regarding the other members of the RNA interference machinery; specially Argonaute proteins which acts as the major stabilizing factor for execution of the interference. This work focuses on the exploration of the sequenced crop genomes available on the web using a hybrid approach of computational protein fishing and genome mining. The results indicate that this hybrid approach was successful in the identification of argonaute proteins in the crop genomes under study.
4

Odyssey of Agrobacterium T-DNA

75%
Ziemienowicz A.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 3
Agrobacterium tumefaciens, a plant pathogen, is characterized by the unique feature of interkingdom DNA transfer. This soil bacterium is able to transfer a fragment of its DNA, called T-DNA (transferred DNA), to the plant cell where T-DNA is integrated into the plant genome leading to "genetic colonization" of the host. The fate of T-DNA, its processing, transfer and integration, resembles the journey of Odysseus, although our hero returns from its long trip in a slightly modified form.
5

Unintended consequences of plant transformation: a molecular insight

75%
Filipecki M., Malepszy S.
Journal of Applied Genetics
|
2006
|
tom 47
|
nr 4
Plant genomes are dynamic structures having both the system to maintain and accurately reproduce the information encoded therein and the ability to accept more or less random changes, which is one of the foundations of evolution. Crop improvement and various uncontrolled stress factors can induce unintended genetic and epigenetic variations. In this review it is attempted to summarize factors causing such changes and the molecular nature of these variations in transgenic plants. Unintended effects in transgenic plants can be divided into three main groups: first, pleiotropic effects of integrated DNA on the host plant genome; second, the influence of the integration site and transgene architecture on transgene expression level and stability; and third, the effect of various stresses related to tissue handling, regeneration and clonal propagation. Many of these factors are recently being redefined due to new researches, which apply modern highly sensitive analytical techniques and sequenced model organisms. The ability to inspect large portions of genomes clearly shows that tissue culture contributes to a vast majority of observed genetic and epigenetic changes. Nevertheless, monitoring of thousands transcripts, proteins and metabolites reveals that unintended variation most often falls in the range of natural differences between landraces or varieties. We expect that an increasing amount of evidence on many important crop species will support these observations in the nearest future.
6

Isolation, cloning and characterisation of motifs containing [GA-TC] repeats isolated from vetch, Vicia bithynica

75%
Sakowicz T., Bowater R., Parniewski P.
Cellular and Molecular Biology Letters
|
2004
|
tom 09
|
nr 3
Microsatellites are widely distributed in plant genomes and comprise unstable regions that undergo mutational changes at rates much greater than that observed for non-repetitive sequences. They demonstrate intrinsic genetic instability, manifested as frequent length changes due to insertions or deletions of repeat units. Detailed analysis of 1600 clones containing genomic sequences of Vicia bithynica revealed the presence of microsatellite repeats in its genome. Based on the screening of a partial DNA library of plasmids, 13 clones harbouring (GA/TC)n tracts of various lengths of repeated motif were identified for further analysis of their internal sequence organization. Sequence analyses revealed the precise length, number of repeats, interruptions within tracts, as well as sequence composition flanking the repeat motifs. Representative plasmids containing different lengths of (GA/TC)n embedded in their original flanking sequence were used to investigate the genetic stability of the repeats. In the study presented herein, we employed a well characterised and tractable bacterial genetic system. Recultivations of Escherichia coli harbouring plasmids containing (GA/TC)n inserts demonstrated that the genetic instability of (GA/TC)n microsatellites depends highly on their length (number of repeats). These observations are in agreement with similar studies performed on repetitive sequences from humans and other organisms.
7

Methylation-sensitive restriction endonuclease digestion patterns revealed in Vicia faba L. chromosomes by in situ nick-translation

75%
Olszewska M. J., Gernand D., Sakowicz T.
Folia Histochemica et Cytobiologica
|
1999
|
tom 37
|
nr 4
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Annotating a non-model plant genome – a study on the narrow-leafed lupin

63%
Zielezinski A., Potarzycki P., Ksiazkiewicz M., Karlowski W. M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2012
|
tom 93
|
nr 3
9

Wplyw genomu i cytoplazmy pszenicy na krzyzowalnosc oktoploidalnego pszenzyta z diploidalnym zytem

51%
Stefanowska G.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
|
1992
|
nr 183
95-98
10

Modyfikacje heterochromatynowe genomu zyta [ S.cereale L.] w wypelnionych i pomarszczonych ziarniakach pszenzyta heksaploidalnego jarego

51%
Pilch J.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
|
1993
|
tom 187
107-112
11

Metody molekularne stosowane w analizie genomu żyta

51%
Pyza A., Kubicka H., Wolska-Sobczak A.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2010
|
tom 555
Opracowanie i wprowadzenie nowocześniejszych metod badawczych, przyczynia się do dokładnego poznania zmienności genetycznej genomów roślinnych. Analiza molekularna na poziomie DNA pozwala ocenić zmienność genetyczną z pominięciem obserwacji fenotypów i wyeliminowaniu wpływu środowiska, które często modyfikuje ekspresję genów. Zastosowanie markerów molekularnych umożliwia bezpośrednie wykrywanie różnic pomiędzy allelami danego genu. Spośród wielu opisanych metod molekularnych, najczęściej do analizy genomu roślinnego wykorzystywane są następujące techniki: PCR, RAPD, RFLP, AFLP oraz mikrosatelity SSR i ISSR. Wymienione metody molekularne DNA są stosowane również z dużym powodzeniem w badaniach genetycznych nad żytem.
12

Arabidopsis thaliana - modelowa roslina nie tylko w biologii molekularnej

51%
Maluszynska J.
Acta Biologica Silesiana
|
1997
|
tom 31
7-16
13

En-Spm-like transposons in Poaceae species: transposase sequence variability and chromosomal distribution

51%
Altinkut A., Raskina O., Nevo E., Belyayev A.
Cellular and Molecular Biology Letters
|
2006
|
tom 11
|
nr 2
Belonging to Class II of transposable elements, En/Spm transposons are widespread in a variety of distantly related plant species. Here, we report on the sequence conservation of the transposase region from sequence analyses of En/Spm-like transposons from Poaceae species, namely Zingeria biebersteiniana, Zingeria trichopoda, Triticum monococcum, Triticum urartu, Hordeum spontaneum, and Aegilops speltoides. The transposase region of En/Spm-like transposons was cloned, sequenced, and compared with equivalent regions of Oryza and Arabidopsis from the gene bank database. Southern blot analysis indicated that the En/Spm transposon was present in low (Hordeum spontaneum, Triticum monococcum, Triticum urartu) through medium (Zingeria bieberstiana, Zingeria trichopoda) to relatively high (Aegilops speltoides) copy numbers in Poaceae species. A cytogenetic analysis of the chromosomal distribution of En/Spm transposons revealed the concurence of the chromosomal localization of the En/Spm clusters with mobile clusters of rDNA. An analysis of En/Spm-like transposase amino acid sequences was carried out to investigate sequence divergence between 5 genera — Triticum, Aegilops, Zingeria, Oryza and Arabidopsis. A distance matrix was generated; apparently, En/Spm-like transposase sequences shared the highest sequence homology intra-generically and, as expected, these sequences were significantly diverged from those of O. sativa and A. thaliana. A sequence comparison of En/Spm-like transposase coding regions defined that the intra-genomic complex of En/Spm-like transposons could be viewed as relatively independent, vertically transmitted, and permanently active systems inside higher plant genomes. The sequence data from this article was deposited in the EMBL/GenBank Data Libraries under the accession nos. AY707995-AY707996-AY707997-AY707998-AY707999-AY708000-AY708001-AY708002-AY708003-AY708004-AY708005-AY708005-AY265312.
14

Effect of diets containing tubers of genetically modified potatoes on parameters of non-specific resistance in rats

51%
Kosieradzka I., Winnicka A., Klucinski W., Sawosz E., Szopa J.
Annals of Animal Science. Supplement
|
2003
|
nr 2
15

The bacterial artificial chromosome [BAC] library of the narrow-leaved lupin [Lupinus angustifolius L.]

51%
Kasprzak A., Safar J., Janda J., Dolezel J., Wolko B., Naganowska B.
Cellular and Molecular Biology Letters
|
2006
|
tom 11
|
nr 3
The narrow-leafed lupin possesses valuable traits for environment-friendly agriculture and for the production of unconventional agricultural products. Despite various genetic and environmental studies, the breeding of improved cultivars has been slow due to the limited knowledge of its genomic structure. Further advances in genomics require, among other things, the availability of a genomic DNA library with large inserts. We report here on the construction of the first DNA library cloned in a BAC (bacterial artificial chromosome) vector from diploid Lupinus angustifolius L. cv. Sonet. The high molecular weight DNA used for its preparation was isolated from interphase nuclei that were purified by flow cytometry. The library comprises 55,296 clones and is ordered in 144×384-well microtitre plates. With an average insert size of 100 kb, the library represents six haploid genome equivalents. Thanks to the purification of the nuclei by flow cytometry, contamination with chloroplast DNA and mitochondrial DNA was negligible. The availability of a BAC library opens avenues for the development of a physical contig map and positional gene cloning, as well as for the analysis of the plant’s genome structure and evolution.
16

Tajemnice funkcjonowania organizmów roślinnych i praktyka ogrodnicza

38%
Malepszy S.
Agricola. Pismo SGGW
|
2011
|
nr 80
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