Wyniki wyszukiwania - AGRO

1

Distribution of eosinophils along and across successive segments of the rat digestive tract: a quantitative study

100%

Furgal A., Litwin J. A.

Folia Histochemica et Cytobiologica

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1997

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tom 35

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nr 3

2

The role of vacuolar digestive system in preventing cadmium poisoning in Acanthamoeba castellanii

100%

Przelecka A., Kluska A. M., Zwierzyk M.

Folia Histochemica et Cytobiologica

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1993

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tom 31

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nr 2

3

Endoscopic examination of the anterior part of digestive tract in cats

100%

Nicpon J., Kubiak K., Sapikowski G.

Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine

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2000

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tom 03

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nr 1

In case of small animals the endoscopic examination of the digestive tract may apply to the endoscopy of esophagus, stomach and duodenum, which is described as the examination of the anterior part of the digestive tract (panendoscopy), or may be limited to examination of rectum and colon, which is described as endoscopy of the posterior part of the digestive tract. The examination was performed in the 12 cats of European race, various sex, 2-6 years of age and divided into 2 groups. We received some interesting results: Fibroscopy of the anterior part of digestive tract is a safe and very useful diagnostic technique to recognise esophagus, stomach and duodenum illnesses in cats. Olympus XQ 20 pediatric fibroscope can be used in endoscopy of the anterior part of digestive tract in cats. Esophagoscopy might be performed with stiff endoscope, which is of little use in gastroscopy.

4

Gastrointestinal (GI) tract-derived Bacteroides fragilis neurotoxins and inflammatory neurodegeneration

88%

Walter L., Zhao Y., Jaber V., Percy M., Taylor C., Aleksandrov P. N.

Acta Neurobiologiae Experimentalis

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2019

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tom 79

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nr Suppl.1

The Gram-negative facultative anaerobe Bacteroides fragilis (B. fragilis) constitutes an appreciable proportion of the human gastrointestinal (GI)‑tract microbiome. As is typical of most Gram-negative bacilli, B. fragilis secretes an unusually complex mixture of neurotoxins including 2 extremely pro-inflammatory species: (i) a B. fragilis‑associated lipopolysaccharide BF‑LPS; and (ii) a B. fragilis-derived proteolytic enterotoxin known as fragilysin (EC 3.4.24.74). BF‑LPS has recently been shown to be associated with the periphery of neuronal nuclei in sporadic Alzheimer’s disease (AD) brain; and the extracellular zinc metalloprotease fragilysin (i) induces endogenous E-cadherin cleavage thereby disrupting cell-cell adhesion and the GI‑tract‑blood barrier (GTBB); and (ii) promotes the generation of the inflammatory transcription factor NF‑kB (p50/p65 complex) in human neuronal‑glial cells in primary culture. In turn, the NF‑kB (p50/p65 complex) strongly induces the transcription of a small family of pro-inflammatory microRNAs (miRNAs) including miRNA‑9, miRNA‑34a, miRNA‑125b, miRNA‑146a, and miRNA‑155. These ultimately bind with the 3’‑untranslated region (3’‑UTR) of several target messenger RNAs (mRNAs) and thereby reduce their expression. Down‑regulated mRNAs include those encoding complement factor-H (CFH), an SH3-proline-rich multi domain-scaffolding protein of the postsynaptic density (SHANK3), and the triggering receptor expressed in myeloid/microglial cells (TREM2), as is observed in sporadic AD brain. Hence, a LPS and an enterotoxic metalloprotease normally confined to the GI tract are capable of driving a disruption in the GI-tract-blood barrier and a NF-kB-miRNA-mediated deficiency in gene expression that contributes to alterations in synaptic-architecture and synaptic-deficits, amyloidogenesis, innate-immune defects, and progressive inflammatory signaling, all of which are characteristic of AD‑type neurodegeneration. This paper will review the most recent research which supports the concept that bacterial components of the GI-tract microbiome such as BF-LPS and fragilysin can transverse normally protective biophysical barriers and contribute to AD‑type changes. For the first‑time, these results indicate that specific GI-tract microbiome-derived neurotoxins have a strong pathogenic potential in disrupting the GI-tract blood barrier and eliciting alterations in NF-kB-miRNA-directed gene expression that drive the AD process.

5

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Endoscopic removal of ingested foreign bodies - Analysis of two cases

88%

Wieczorski M., Prystupa A., Falkowska A., Piechota G.

Journal of Pre-Clinical and Clinical Research

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2015

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tom 09

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nr 2

Introduction and Objective. The study describes the clinical characteristics of two patients who underwent endoscopic removal of a foreign body from the GI tract. Thea in was comparative analysis of endoscopy and other methods of treatment. Materials and method. The cases of two patients who presented to the ED with complaints related to the swallowing of foreign bodies (dentures) were retrospectively reviewed. Diagnostic methods, treatment, risk factors and clinical outcomes were analyzed. Conclusions. In most cases, endoscopy is an effective method of foreign body removal; however, some patients may require other treatment (eg. patients with risk factors or location/position of foreign body that cannot be treated by endoscopy). Endoscopy is also safe, available and a relatively quick procedure.

6

Reefs in experimental gastroenterology - cyclic activities of the gastrointestinal tract

88%

Zabielski R.

Journal of Physiology and Pharmacology. Supplement

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2004

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tom 55

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nr 2

19-32

The activity of the upper gastrointestinal tract is periodic. It concerns the gastrointestinal and gallbladder motility, gastrointestinal blood flow, gastric, intestinal, pancreatic and biliary secretions, rate of nutrient absorption, and many other physiological events. Nowadays, the periodic activity of the gastrointestinal tract is considered as a basic physiological pattern in conscious animals and humans. Unfortunately, there are considerable species- and age-related as well as individual differences, therefore experimental protocols should consider first describing the individual periodic pattern in the examined animals. A lot of confusion may appear with data interpretation if the periodic activity is neglected, in particular when low physiological-like doses of test substances are used. For instance, the effect of CCK or VIP administration on the exocrine pancreas may differ from negligible effect to strong one depending of the phase of pancreatic secretion. The action of secretagogues on the gastrointestinal tract will also be discussed in terms of the ultradian and circadian cycles.

7

Expression of class III beta-tubulin in neuroendocrine tumours of gastrointestinal tract

88%

Jirasek T., Mandys V., Viklicky V.

Folia Histochemica et Cytobiologica

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2002

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tom 40

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nr 3

8

Synthesis and macromolecular organization of gastrointestinal mucin

88%

Slomiany A., Slomiany B. L.

Journal of Physiology and Pharmacology

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1992

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tom 43

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nr 2

Mucus glycoproteins (mucins), the principal determinants of mucus protective qualities and mucosal defense, are studied extensively to define pathological aberrations in the relation to gastrointestinal disease and to develop the mucous barrier strengthening agents. Recent work from our laboratory provided evidence as to the initial stages of the gastrointestinal mucin synthesis, molecular size of the apomucin, its macromolecular organization and interaction with other elements of gastrointestinal mucus. Using monoclonal antibodies against apomucin (clone 1H7), O- glycosylated with N-acetylgalactosamine apomucin (clone 2B4), and that against carboxyl terminal of the apomucin (clone 3G12), the mucin synthesizing polysomes were isolated and glycosylated peptides ranging in size from 6-60kDa identified. The in vitro synthesis in the cell-free system also afforded 60-64kDa products recognized by 1H7 and 3G12 antimucin MAbs. The obtained results provided evidence that the mucin core consists of 60kDa peptide which at cotranslational stage is O-glycosylated with N-acetylgalactosamine. Studies on mucin polymer assembly revealed that mucin preparations prepared by equilibrium density gradient centrifugation and Sepharose 2B chromatography (Mantle, M., Mantle, D., and Allen, A. (1981) Biochem. J. 195, 277-285) are not completely purified and contain DNA and extraneous proteins. The evidence was obtained that so called mucin “link protein”, 118kDa glycopeptide, is a N-glycosylated fragment of fibronectin, whereas the supposedly native undegraded mucin isolated by Carlstedt et al. (Biochem. J. (1983) 211, 13-22) was found to contain mucin-fibronectin-DNA complexes. The general picture that emerged from the studies is that the pure mucin consists of 60kDa glycosylated peptides only. The carboxyl terminal (8-12kDa fragment) of these peptides is not glycosylated (naked) and is responsible for mucin interaction with fibronectin and other fibronectin-like extracellular matrix proteins. While the formation of the mucosal coat depends on many other factors and extracellular components, our findings on mucin structure and interaction with the extracellular matrix proteins provide explanation as to the possible mechanism of mucin adherence to the epithelial surfaces.

9

Comparison of aspartate proteinase activity of Candida albicans strains isolated from children with and without Helicobacter pylori

88%

Rozga A., Kurnatowski M., Wasowska-Krolikowska K.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 3

10

Occurrence of parasitic and other disorders of the alimentary tract of the clinic for enteric diseases

88%

Lach J., Golinska Z., Bany J.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 3

11

Role of histamine H3 receptors in the regulation of gastric functions

88%

Coruzzi G., Morini G., Adami M., Grandi D.

Journal of Physiology and Pharmacology

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2001

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tom 52

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nr 4,1

The role of central and peripheral histamine H3 receptors in the regulation of gastric acid secretion and gastric mucosal integrity is reviewed. The activation of H3 receptors by peripheral administration of the selective agonist (R) alpha-methylhistamine reduced acid secretion in cats, dogs, rats and rabbits, while increasing it in mice. The antisecretory effects were observed against indirect stimuli that act on vagal pathways or on enterochromaffin-like (ECL) cells, such as 2-deoxy-D-glucose, food or pentagastrin, but not against histamine or dimaprit. Inhibitory effects on acid production were observed in rats after central administration of histamine or of H3 receptor agonists. In the conscious rat intragastric administration of (R) alpha-methylhistamine caused gastroprotective effects against the damage induced by absolute ethanol, HCl, aspirin and stress. The mechanism involved seems to be related to the increased mucus production, via nitric oxide-independent mechanisms. Gastroprotective effects against ethanol were also observed after central administration of histamine or its metabolite Nalpha -methylhistamine, suggesting that brain H3 receptors participate in the histamine-mediated effects on gastric functions.

12

Geotrichum candidum Link 1809: hydrolases activity and own methoof digestive tract strains biotyping

88%

Kurnatowska A., Bialasiewicz D.

Wiadomości Parazytologiczne

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2001

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tom 47

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nr 4

Hydrolase activity of (enzymograms, biotypes) in Geotrichum candidum, one of the poorly described pathogenic fungi, was studied 81 strains were isolated from oral cavity and faeces of patients with gastrointestinal tract disorders. Axenic strains were differentiated with API 20C Aux and API ZYM tests. Then, enzymograms and biotypes were determined for all strains based on the activity of 19 hydrolases. High variability of enzymograms (17 different types) was found. The highest activity was noted in case of: e2-alkaline phosphatase, e6-lecucine arylamidase, e11-acid phosphatase. E5-lipase. e7-valine arylamidase, e12 - naphtol-AS-BI-phosphohydrolase and e17-β-glucosidase were used for biotyping procedures. Our own system of biotyping of 81 strains of G. candidum was based on the mathematical binominal distribution formula (1 : 4 : 6 : 4: 1) – all; ,,+”;one „-”, three „+”; two „-”,two „+”; three ,,-”,one ,,+”; all „-”. We have found: A (11.1 ± 3.5%), B1 (6.17 ± 2.67%). B2 (1.23 ± 1.22%), B4 (4.94 ± 2.41%), C1 (1.23 ±1.22%), C3 (63.0 ± 5.4%), D2 (9.88 ±3.31%), D3 (2.47 ± 1.72%). Among all strains from 8 various biotypes of G. candidum.

13

Candida albicans Berthout, 1923: Hydrolases activity and own method of digestive tract strains biotyping

88%

Kurnatowski M., Wasowska-Krolikowska K., Kurnatowska A.

Wiadomości Parazytologiczne

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2002

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tom 48

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nr 4

112 strains of Candida albicans were isolated from oral cavity and ontocenoses of the upper digestive tract (endoscopy) of children (age: 5-17) with gastrointestinal disorders. Axenic strains were differentiated with API 20C AUX and API ZYM tests (bioMerieux). Then enzymograms and biotypes were determined for all the strains based on the activity of 19 hydrolases. The highest activity was noted for: e₂ - phosphatase alcaline, e₆ – leucine arylamidase, e₁₁ - phosphatase acid, e₅ - lipase (c₁₄); e₇ - valine arylamidase, e₁₂ - naphtol-AS-BI-phosphohydrolase, e₁₆ - α-glucosidase and e₁₈ - N-acetyl-ß-glucosamidase, and the latter four were used for biotyping procedures. Our own system was based on the mathematical binominal distribution formula (1: 4: 6: 4: 1): all „+”; one „ -” three „+”; two „-”, two ,,+”; tbree „-”, one „+”; all „-”. We bave found the following biotypes: A (16.1 ± 3.5%), B₁ (2.7 ± 2.53%), B₃ (8.0 ± 25%), B₄ (22.3 ± 3.9%), C₂ (1.8 ± 1.3%), C₃ (7.1 ± 2.4%), C₆ (30.4 ± 4.3%), D₃ (11.6 ± 3.0%).

14

The immunology of the gastrointestinal tract. Contemporary views

88%

Sowinski R., Zielinski A.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

15

The effect of transcutaneous nerve stimulation (TENS) on gastric electrical activity

88%

Furgala A., Thor P. J., Kolasinska-Kloch W., Krygowska-Wajs A., Kopp B., Laskiewicz J.

Journal of Physiology and Pharmacology

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2001

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tom 52

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nr 4,1

TENS became widely accepted method of treatment pain syndromes in clinical practice. Lately has been shown that its affects also gastrointestinal tract by releasing NANC neurotransmitter VIP. The aim of this study was to evaluate the effects of TENS on gastric myoelectric activities measured by electrogastrography (EGG). Eighteen healthy men (mean age 23 1.7) were included in the study. Healthy volunteers were divided on 3 groups each 6 persons: with normogastria occurring at 94.5 7% of recording time — group A, with predominant bradygastria (36.6 ± 14%) — group B and with tachygastria (33 14%) — group C. In fasted condition EGG (Synectics, Sweden) was recorded with skin electrodes. TENS 15 min was performed with use of Sinus 5 stimulator (6 Hz, 0.1ms duration, intensities 10 — 20mA, Zimmer, Germany). Stimulating electrodes were placed on non-dominant hand. Results: None of the subjects during TENS reported any side effects or symptoms, during the all studies. In group A in the fasting recordings, after TENS, an decrease of the normal values in the range 2 — 4cpm down to 78.5 ± 21% of recording time (p = 0.03) occurred. The dominant frequency in the bradygastric region increased up to 17.7 7% of the total recording. In group B TENS decreased bradygastria level from 36.6 14% to 20.6 15% (p = 0.02). TENS did not significantly affect tachygastria in group C. Amplitude of the EGG signal after TENS in group B and C increased by 40 and 150% respectively (p < 0.05). Significant decrease of the amplitude was observed in group A (13%). We conclude that TENS by activating centrally mediated somato-visceral reflexes affects gastric electrical activity. Our results suggest that TENS may be useful in treatment of the gastric dysrhythmia.

16

Providencia alcalifaciens as the presumptive cause of diarrhoea in dog

75%

Krol J., Janeczek M., Pliszczak-Krol A., Janeczek W., Florek M.

Polish Journal of Veterinary Sciences

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2007

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tom 10

|

nr 4

A case of diarrhoea in a four-month-old golden retriever is described. On the basis of anamnesis and bacteriological examination, the diagnosis of bacterial enteritis due to Providencia alcalifaciens was reached. Although there are contradictory opinions about the role of this organism as the enteric pathogen, it seems that Providencia alcalifaciens should also be taken into consideration in the routine bacteriological diagnostics of diarrhoea in dogs.

17

Allometric growth of the alimentary tract in the Siberian hamster (Phodopus sungorus)

75%

Wolczuk K., Pawlak R.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2014

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tom 56

|

nr 1

18

Colistin resistance of non-pathogenic strains of Escherichia coli occurring as natural intestinal flora in broiler chickens treated and not treated with colistin sulphate

75%

Majewski M., Lukomska A., Wilczynski J., Wystalska D., Racewicz P., Nowacka-Woszuk J., Pszczola M., Anusz K.

Journal of Veterinary Research

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2020

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tom 64

|

nr 3

19

Features of pharmacotherapy of hepatic encephalopathy manifestations in liver cirrhosis

75%

Maretskyi V., Lobanets N.

Health Problems of Civilization

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2015

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tom 09

|

nr 2

The aim of this work is to study the effect of drug mebikar on the clinical manifestations of hepatic encephalopathy in patients with liver cirrhosis. The study involved 34 patients with cirrhosis of different etiologies. The average age of the examined patients was (48.5±0.9) years, that prevailed patients of working age, indicating the medical and social significance of the problem of early diagnosis and adequate treatment of cirrhosis. One of the most frequent complications of cirrhosis is hepatic encephalopathy. In addition to conventional clinical and laboratory findings in patients with liver cirrhosis, severities of hepatic encephalopathy were determined according to West-Haven criteria before and after treatment. In patients with liver cirrhosis was established the presence of latent or clinically expressed hepatic encephalopathy. The treatment of the control group of patients consisted of the following drugs: essential phospholipids, mixture of sorbitol and major ions, arginine glutamate, furosemide, verospiron, lactulose, amoxicillin trihydrate and lansoprazole. In the complex treatment of the main group of patients medicine mebikar was administered additionally. Analysis of the clinical manifestations of hepatic encephalopathy showed a marked improvement in patients who received additional treatment with mebikar. Specifically, the incidences of mood changes as well as anxiety decreased in this group on average of 38% compared with those patients without an additional treatment with mebikar. Also, sleep disturbances in the main group was observed to be lower by 7.2% compared to those in the control group. Inclusion in the treatment of patients with liver cirrhosis, the drug mebikar – a daytime tranquilizer with anxiolytic properties reduces neurotic disorders, improves emotional state which may indicate a regression in the manifestations of hepatic encephalopathy thereby improving the quality of life of patients and thus substantiating an expedient inclusion of mebikar an anxiolytic drug to the complex therapy of patients with liver cirrhosis.

20

Investigation of isolated Blastocystis subtypes from cancer patients in Turkey

75%

Mulayim S., Aykur M., Dagci H., Dalkilic S., Aksoy A., Kaplan M.

Acta Parasitologica

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2021

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tom 66

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nr 2

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