Eyespot caused by Oculimacula acuformis and O. yallundae is a common, economically important disease of cereals in Poland. For the pathogens control fungicides like triazoles, imidazoles, strobilurins, morpholines, anilino-pyrimidines, pyridine carboxamide, and benzimidazoles are used. However, intense use of the same active compounds leads to the appearance of resistant fungal strains. The aim of the study was to evaluate under laboratory conditions the resistance level of O. acuformis and O. yallundae isolates to fungicides used in the cereal protection against eyespot. Boscalid, prochloraz, pyraclostrobin, epoxiconazole and flusilazole were the compounds highly limiting growth of the isolates on PDA (Potato Dextrose Agar) medium. The group that included tebuconazole, cyprodinil, kresoxim-methyl and fenpropimorph inhibited the growth of fungal isolates quite well. The laboratory test results indicate the possibility of effectively control of eyespot with the tested fungicides with the exception of benzimidazoles (carbendazim and thiophanate-methyl), as most of the tested isolates of Oculimacula spp. isolates were resistant to the products containing these active ingredients.