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Analysis of plant tissue images obtained by confocal tandem scanning reflected light microscope

100%

Gancarz M., Konstankiewicz K., Pawlak K., Zdunek A.

International Agrophysics

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2007

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tom 21

|

nr 1

2

Immunolocalization of disorganized muscle protein-1 in different life stages of human lymphatic flariid, Brugia malayi

86%

Kushwaha V., Sahasrabuddhe A. A., Murthy P. K.

Acta Parasitologica

|

2020

|

tom 65

|

nr 4

3

The effect of process variablles on the NSSC pulp chemical composition-the use of confocal microscopy

86%

Joachimiak K., Wojciak A., Gagat M.

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

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2012

|

tom 78

4

Gap junction density in human myometrium at term revealed by an anti-peptide antibody and laser scanning confocal microscopy

86%

Kilarski W. M., Severs N. J., Gourdie R. G., Rezapour M., Backstrom T., Roomans G. M., Ulmsten U.

Folia Histochemica et Cytobiologica

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1993

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tom 31

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nr 4

5

New confocal microscopies and their analytical capabilities

86%

Dobrucki J. W.

Biological Letters

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2005

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tom 42

|

nr 2 Spec.Vol.

6

A search for colocalization of serotonin 5-HT2A and 5-HT1A receptors in the rat medial prefrontal and entorhinal cortices-immunohistochemical studies

86%

Wedzony K., Chocyk A., Mackowiak M.

Journal of Physiology and Pharmacology

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2008

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tom 59

|

nr 2

Recently developed antipsychotic drugs ameliorating the negative symptoms of schizophrenia act not only on dopamine D2 receptors but also on serotonin 2A (5-HT2A) and 1A (5-HT1A) receptors in specific regions of the cerebral cortex. Since it is not yet known whether serotonin 5-HT1A and 5-HT2A receptors coexist in the same population of neurons in the cortex, the present study investigated their colocalization in the rat medial prefrontal (MPC) and entorhinal (EC) cortices. Using antibodies that recognize epitopes specific to the serotonin 5-HT2A or 5-HT1A receptors, studies employing confocal microscopy have shown that in the MPC 5-HT2A receptors are preferentially, if not exclusively, present on the pyramidal neurons and that 5-HT1A-immunopositive material is present in the axonal hillocks and, to lower extend, in cytoplasm of presumably pyramidal cell bodies. With the regard of labeling of active receptors (i.e. present in shafts and axonal hillocks) we found that about 38% of neurons positive for the presence of serotonin 5-HT2A receptors, are also positive for serotonin 5-HT1A receptors in the MPC. In the EC, only 22% of serotonin 5-HT2A-positive neurons were positive for serotonin 5-HT1A receptor-immunoreactivity. In the respect of cytoplasmatic serotonin 5-HT1A receptor-immunoreactivity (possibly inactive receptors), 65% and 73% of serotonin 5-HT2A receptor-positive neurons were colocalized with serotonin 5-HT1A receptors in the MPC and EC, respectively. Data obtained on serotonin 5-HT2A and 5-HT1A receptor localization provide anatomical grounds for at least three distinct populations of pyramidal neurons, one governed only by 5-HT2A, one only by 5-HT1A and one by both types of serotonin receptors.

7

Analysis of multidimensional digital images in fluorescence and confocal microscopy

86%

Dobrucki J. W.

Cellular and Molecular Biology Letters

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2002

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tom 07

|

nr Suppl.

8

The interactions between SATB1 and F-actin are important for mechanisms of active cell death

72%

Grzanka D., Kowalczyk A. E., Izdebska M., Klimaszewska- -Wisniewska A., Gagat M.

Folia Histochemica et Cytobiologica

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2015

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tom 53

|

nr 2

9

Mechanisms involved in the development of the small intestine mucosal layer in postnatal piglets

72%

Skrzypek T. H., Kazimierczak W., Skrzypek H., Valverde Piedra J. L., Godlewski M. M., Zabielski R.

Journal of Physiology and Pharmacology

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2018

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tom 69

|

nr 1

10

Caveolin-1 localization in chicken embryo chorioallantoic membrane treated with diamond and graphite nanoparticles

72%

Wierzbicki M., Sawosz E., Grodzik M.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

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2012

|

tom 51

Caveolin--1 is a multifunctional protein and major component of caveolae membranes that participates in regulation of signaling pathways, endocytosis and molecular transport. Caveolin-1 takes part in regulation of angiogenesis regulation signaling pathways. Diamond nanoparticle have been shown to inhibit development of blood vessel. Molecular mechanism of diamond nanoparticles anti-angio-genic activity can be related with interactions with cellular membranes. The objective of this experiment is to verify effect of carbon nanoparticles on morphology of highly vascularized chicken embryo chorioallantoic membrane (CAM) and caveolin-1 intracellular localization. In this study two types of carbon nanoparticles were used: diamond nanoparticles (ND) and graphite nanoparticles (NG), which are similar in size (3-5 nm) but different in molecular structure. At day six of chicken embryo embryonic development sterile implant of diameter 10 mm made from Waterman filter paper ware placed on chicken embryo CAM. At day seven of embryonic development implants with CAM were subjected to further analyzes. CAM cross-sections were immuno-localized with anti caveolin-1 antibody and visualized by confocal microscope. Three dimensional analysis of chorion membranes show that ND, but no NG change intracellular distribution of caveolin-1. Furthermore ND decreases density of mesenchymal cells and extracellular matrix collagen fibers

11

Muscular arrangement and sclerite morphology in the haptor of Tetraonchus monenteron (Monogenea, Dactylogyridea)

72%

Petrov A. A., Gerasev P.

Acta Parasitologica

|

2019

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tom 64

|

nr 1

12

Cytotoxicity of PP(Arg)2- and Hp(Arg)2-mediated photodynamic therapy and early stage of apoptosis induction in prostate carcinoma in vitro

72%

Nowak-Stepniowska A., Wiktorska K., Malecki M., Romiszewska A., Padzik-Graczyk A.

Acta Biochimica Polonica

|

2011

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tom 58

|

nr 4

Porphyrin photosensitizers tend to localize in mitochondria. The depolarization of mitochondrial membrane is one of the early stages of apoptosis and Laser Scanning Fluorescence Microscopy allows to determine changes in transmembrane mitochondrial potential under influence of PDT depending on the kind of photosensitizer (PP(Arg)2, Hp(Arg)2), the energy dose (5, 10, 30 and 50 J/cm2) and time periods (24 and 48 hours after irradiation) in the LNCaP (lymphonodal metastasis of prostate carcinoma, the androgen dependent cell line). Cyototoxicity induced by PP(Arg)2- and Hp(Arg)2-based PDT depending on energy dose and time after irradiation in prostate carcinoma is determined with MTT. Generally, it was shown that lower energy doses induce greater changes in transmembrane mitochondrial potential. Hp(Arg)2-based PDT was more effective causing greater mitochondrial membrane depolarization and cell viability decrease in comparison to PP(Arg)2-mediated PDT (in the case of maximal nontoxic photosensitizer doses used).

13

The confocal microscopy, the tool for rapid in vivo diagnosis of Acanthamoeba keratitis - usefulness and limitations

72%

Chomicz L., Izdebska J., Padzik M., Baltaza W., Pionkowski K., Jasniewska E., Dybicz M.

Annals of Parasitology

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2016

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tom 62

|

nr Suppl.

14

Subcellular localization of full-length human myeloid leukemia factor 1 (MLF1) is independent of 14-3-3 proteins

72%

Molzan M., Ottmann C.

Cellular and Molecular Biology Letters

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2013

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tom 18

|

nr 1

Myeloid leukemia factor 1 (MLF1) is associated with the development of leukemic diseases such as acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). However, information on the physiological function of MLF1 is limited and mostly derived from studies identifying MLF1 interaction partners like CSN3, MLF1IP, MADM, Manp and the 14-3-3 proteins. The 14-3-3-binding site surrounding S34 is one of the only known functional features of the MLF1 sequence, along with one nuclear export sequence (NES) and two nuclear localization sequences (NLS). It was recently shown that the subcellular localization of mouse MLF1 is dependent on 14-3-3 proteins. Based on these findings, we investigated whether the subcellular localization of human MLF1 was also directly 14-3-3-dependent. Live cell imaging with GFP-fused human MLF1 was used to study the effects of mutations and deletions on its subcellular localization. Surprisingly, we found that the subcellular localization of full-length human MLF1 is 14-3-3-independent, and is probably regulated by other as-yet-unknown proteins.

15

Somatic embryogenesis of a tree fern Cyathea delgadii Sternb.: achievements and prospects

72%

Mikula A., Grzyb M., Pozoga M., Tomiczak K., Domzalska L., Rybczynski J. J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

|

tom 96

|

nr 1

16

Establishing and functional characterization of an HEK-293 cell line expressing autofluorescently tagged beta-actin [pEYFP-actin] and the neurokinin type 1 receptor [NK1-R]

72%

Hrovat A., Zavec A. B., Pogacnik A., Frangez R., Vrecl M.

Cellular and Molecular Biology Letters

|

2010

|

tom 15

|

nr 1

55-69

This study focused on establishing and making a comprehensive functional characterization of an HEK-293-transfected cell line that would coexpress the enhanced yellow fluorescent protein-actin (pEYFP-actin) construct and the neurokinin type 1 receptor (NK1-R), which is a member of the seven transmembrane (7TM) receptor family. In the initial selection procedure, the cloning ring technique was used alone, but failed to yield clones with homogenous pEYFP-actin expression. Flow cytometry sorting (FCS) was subsequently used to enrich the pEYFP-actin-expressing subpopulation of cells. The enzyme-linked immunosorbent assay (ELISA), FCS and quantitative real-time reverse transcription/polymerase chain reaction (RT-PCR) were then employed to monitor the passage-dependent effects on transgene expression and to estimate the total β-actin/pEYFP-actin ratio. NK1-R was characterized via radioactive ligand binding and the second messenger assay. The suitability of the pEYFP-actin as a marker of endogenous actin was assessed by colocalizing pEYFP-actin with rhodamine-phalloidine-stained F-actin and by comparing receptor- and jasplakinolide-induced changes in the actin cytoskeleton organization. These experiments demonstrated that: i) both constructs expressed in the generated transfected cell line are functional; ii) the estimated pEYFP-actin: endogenous β-actin ratio is within the limits required for the functional integrity of the actin filaments; and iii) pEYFP-actin and rhodamine-phalloidine-stained F-actin structures colocalize and display comparable reorganization patterns in pharmacologically challenged cells.

17

Comparison of the amount and demonstrability of endogeneous hormones and bound insulin after paraformaldehyde and EDAC fixation in Tetrahymena

72%

Csaba G., Kovacs P., Pallinger E.

Acta Protozoologica

|

2006

|

tom 45

|

nr 4

18

Confocal visualization of the effect of short-term locomotor exercise on BDNF and TrkB distribution in the lumbar spinal cord of the rat: The enhancement of BDNF in dendrites?

72%

Macias M., Dwornik A., Skup M., Czarkowska-Bauch J.

Acta Neurobiologiae Experimentalis

|

2005

|

tom 65

|

nr 2

19

Confocal microscopy for investigations of agricultural materials

72%

Konstankiewicz K., Krol A., Pukos A.

International Agrophysics

|

1995

|

tom 09

|

nr 4

20

Confocal microscopy capabilities, limitations, typical technical problems

72%

Dobrucki J. W.

Biological Letters

|

2005

|

tom 42

|

nr 2 Spec.Vol.

Ograniczanie wyników

Analysis of plant tissue images obtained by confocal tandem scanning reflected light microscope

Immunolocalization of disorganized muscle protein-1 in different life stages of human lymphatic flariid, Brugia malayi

The effect of process variablles on the NSSC pulp chemical composition-the use of confocal microscopy

Gap junction density in human myometrium at term revealed by an anti-peptide antibody and laser scanning confocal microscopy

New confocal microscopies and their analytical capabilities

A search for colocalization of serotonin 5-HT2A and 5-HT1A receptors in the rat medial prefrontal and entorhinal cortices-immunohistochemical studies

Analysis of multidimensional digital images in fluorescence and confocal microscopy

The interactions between SATB1 and F-actin are important for mechanisms of active cell death

Mechanisms involved in the development of the small intestine mucosal layer in postnatal piglets

Caveolin-1 localization in chicken embryo chorioallantoic membrane treated with diamond and graphite nanoparticles

Muscular arrangement and sclerite morphology in the haptor of Tetraonchus monenteron (Monogenea, Dactylogyridea)

Cytotoxicity of PP(Arg)2- and Hp(Arg)2-mediated photodynamic therapy and early stage of apoptosis induction in prostate carcinoma in vitro

The confocal microscopy, the tool for rapid in vivo diagnosis of Acanthamoeba keratitis - usefulness and limitations

Subcellular localization of full-length human myeloid leukemia factor 1 (MLF1) is independent of 14-3-3 proteins

Somatic embryogenesis of a tree fern Cyathea delgadii Sternb.: achievements and prospects

Establishing and functional characterization of an HEK-293 cell line expressing autofluorescently tagged beta-actin [pEYFP-actin] and the neurokinin type 1 receptor [NK1-R]

Comparison of the amount and demonstrability of endogeneous hormones and bound insulin after paraformaldehyde and EDAC fixation in Tetrahymena

Confocal visualization of the effect of short-term locomotor exercise on BDNF and TrkB distribution in the lumbar spinal cord of the rat: The enhancement of BDNF in dendrites?

Confocal microscopy for investigations of agricultural materials

Confocal microscopy capabilities, limitations, typical technical problems