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The aim of this 3-year study was to determine the influence of harvest time on dry matter, total dietary fibre, fractional composition of dietary fibre (acid detergent fibre (ADF) and neutral detergent fibre (NDF)), water-soluble carbohydrates (WSCs) and inulin content in tubers of different cultivars of Jerusalem artichoke (Helianthus tuberosus L.): ‘Rubik’, ‘Albik’ and ‘Sauliai’. The tubers were harvested in spring (March and April) and autumn (October and November). Our study shows that the largest amount of WSC to exist in ‘Rubik’ tubers harvested especially during October. In contrast, the largest amount of total dietary fibres and their fractions (NDF and ADF) accumulated in ‘Albik’ tubers during the spring harvest month (April). The largest amount of inulin was obtained from ‘Sauliai’ tubers harvested in October. Based on our results, it is advisable to use the ‘Sauliai’ tubers from the harvest time during October to obtain the largest amount of inulin.
Most of glycoproteins described so far, including immunoglobulins, are glycosylated during post-translational modifications of protein molecules. Current knowledge of the structure of sugar chains in immunoglobulin molecules and their biological role in health and pathology is reviewed.
Using 6 culture media (12, 12D, 12G, 11, A and B) made up of MS medium (Murashige-Skoog, 1962) supplemented or not with glycerine, with different cytokinins, and/or 2,4-D, the morphological characteristics and contents in total carbohydrates, reducing sugars, sucrose and starch were studied in calli induced from explants (cotyledon, petiole, hypocotyl and leaf) obtained from Medicago strasseri seedlings. Callus formation was induced under photoperiod (16h light/8h darkness) conditions or in the absence of light. Considerable variability in the calli was observed, depending on the explants and media used. Under photoperiod conditions, medium A with KIN (1 mg/l) and 2,4-D (3 mg/l) induced many calli with the highest contents in total carbohydrates (886.1–889.3 mg/g DW), sucrose (132.1–188.2 mg/g DW) and starch (125.2–247.6 mg/g DW) and the lowest contents in reducing sugars (118.4–173.3 mg/g DW). In media 11, A and B, under conditions of darkness, calli degenerated at the start of culture. Calli developed in darkness generally had dry weights and total carbohydrate and starch contents lower than those cultured under photoperiod conditions. However, sucrose contents were greater in calli formed in darkness. At these cultures times, differentiation, in the form of organogenesis, was only seen using medium B with cotyledons, petioles and leaves as explants. It was also observed when petioles were cultured in medium A but with a less pronounced organogenic response.
Background: Glycemic load (GL) is used to evaluate how various food products affect blood sugar level. According to some studies, high dietary GL may increase the risk of cancer development and recurrence. Objective: The aim of the study was to assess dietary glycemic load and intake of carbohydrates derived from various food products by patients staying on an oncological ward. Material and methods: The study group included 100 cancer patients aged 19-83 years (59.6 ± 11.3 years). GL, energy and nutrient intake was estimated based on the data from the Food Frequency Questionnaire (FFQ). The correlations between dietary GL and consumption of 18 groups of products were assessed. Results: The average GL per 1000 kcal was 61.0 ± 8.6 g in the diets of men and 56.2 ± 9.5 g in the diets of women. High GL (>120 g) was observed in 76% of analyzed diets. The diets of men had higher GL, energy and sucrose content than the diets of women. Men, in comparison to women, consumed more refined grain products (144.1 ± 78.2 g vs. 95.5 ± 67.8 g), beverages (236.4 ± 344.7 g vs. 69.2 ± 173.0 g), honey and sugar (28.0 ± 22.2 g vs. 16.7 ± 18.0 g), dark chocolate (4.5 ± 4.5 g vs. 3.9 ± 6.7 g), sweets (66.1 ± 56.6 g vs. 38.8 ± 39.5 g) and soups (313.3 ± 105.3 g vs. 260.8 ± 160.3 g). Conclusions: Analyzed diets were characterized by high GL and simple sugars content. Men consumed more refined and sweetened products than women. The improvement of knowledge about proper nutrition is needed in studied group of cancer patients.
Chemical deglycosylation (dg) of sturgeon Acipenser gueldenstaedti Br. (alphaGTH) resulted in the loss of 83% of its initial carbohydrate content. It altered also recombinant dgalphaGTH + betaGTH dimer molecule, reducing its immunoreactivity by 30%, and fully blocking the hormonal function. CD spectroscopy showed that deglycosylation led to changes in the secondary structure of dgalphaGTH and in the alpha-beta recombinant. The sugar moiety of sturgeon alphaGTH is suggested to play an important role in maintaining the biological function of the hormone dimer molecule.
Variations in lipid, protein and carbohydrate contents of Enteromorpha spp. were examined over a seven-month period from April to October 1993. The samples were collected from seven sampling stations along the Gulf of Gdańsk coast. The lipid content was low and varied slightly from 3.47±1.76% of DW at Puck to 4.36±2.17% of DW at Rewa and Chałupy. The protein content varied from 9.42±4.62% of DW at Puck to 20.60±5.00% of DW at Jurata. At the remaining stations the values vary over a narrow range. The maximum protein contents were recorded at the beginning and end of the growing season. The level of carbohydrate was very high compared to that of lipid and protein and varied from 29.09±6.44% of DW at Osłonino to 39.81±11.15% of DW at Puck. Seasonal carbohydrate changes were noted at all sampling stations, the minimum occurring in spring and autumn and the maximum in summer.
Investigations were carried out to find whether enhanced ultraviolet radiation influences the Taxus, Thuja and Juniperus genera used in garden architecture. Seedlings a few years old were subjected to UV-B at a 16 kJ m-2 day-1 dose for several weeks. carbohydrate contents (monosaccharides and sucrose), levels of chlorophylls and chlorophyll a fluorescence were analyzed, and the variable/maximal fluorescence (Fv/ Fm) and the Rfd _ vitality index were calculated. In Taxus and Juniperus no negative effects were found in carbohydrate accumulation and even increased chlorophyll a and b levels were noted. After 6-9 weeks of irradiation the amount of these pigments in Juniperus needles was as many as twofold higher. Under the in­fluence of uv-B a 50% reduction of monosaccharide accumulation was found only in Thuja, after 3 weeks irradiation, and an approx. 30% reduction in chlorophylls after 6-9 weeks of irradiation. No changes in the Fv/Fm parameter were found in either species, but the vitality index (Rfd) under the first weeks of radiation stress showed even an increase in Thuja leaves. These results indicate that shoot blight of conifers is not caused by enhanced UV-B radiation.
The study were carried out in the years 1996–1998 on the soil characterized as a good rye complex. The experiment was set according to the method of randomized sub blocks at 3 replications. The four following factors were examined: 1) cultivation technologies: a) conventional technology – control, b) with polyethylene sheeting, c) with polypropylene sheeting, d) with double shield of polypropylene and polyethylene sheeting; 2) methods of weed control of potato: a) mechanical, b) with Afalon, c) with Racer, d) with herbicide mixture Afalon + Command; 3) potato cultivars: Aster and Drop; 4) harvest terms: a) 60 days after planting, b) 75 days after planting, c) harvest at full maturity. Coverage application at potato cultivation, as compared to traditional one, contributed to the decrease of dry mass and the increase of starch, total sugars, reducing sugars and sacharose in the potato tubers.
The aim of the present work was to determine the carbohydrate content in plantlets of Clematis pitcheri cultured in vitro depending on the temperature (15°C, 20°C and 25°C) and sucrose (10 and 30 g·dm⁻³) and nitrogen (100% and 50% of normal MS strength) level in the medium. 100% N meant 1.90 g KNO₃ and 1.65 g NH₄NO₃, and 50% N meant 0.950 g KNO₃ and 0.825 g NH₄NO₃ in 1 dm⁻³ of MS medium. The highest accumulation of starch in shoots took place at 30 g·dm⁻³ of sucrose and lower level of nitrogen compounds in the medium. Also, at 30 g·dm⁻³ of sucrose in the medium the content of fructose in shoots was higher and independent from the level of nitrogen. The temperature of 25°C stimulated glucose accumulation in shoots at the highest degree at 30 g·dm⁻³ of sucrose and lower level of nitrogen. Regardless of the treatment, no sucrose was found in plantlets. Generally, the higher concentration of sucrose in the medium the higher content of sum of carbohydrates in plantlets. A lower nitrogen compound level (50% N) led to increase of glucose and starch content in shoots, and had a smaller effect on the level of fructose. Shoots cultured at the temperature of 20°C and 25°C accumulated more carbohydrates than at 15°C.
In this study two sowing date in years 2007-08 (1 November as normal sowing date and 1 January as late sowing date) were used. Measurements of chlorophyll fluorescence, membrane thermo-stability, gas exchange parameters and carbohydrates content in two wheat cultivars (Dena as durum wheat and Roshan as bread wheat) at post- anthesis stage were recorded. Acquired thermo-tolerance showed significant variation in late sown conditions. It was inferred that durum wheat conferred less relative injury and greater thermo-tolerance possibly through maintaining cellular membrane integrity under high temperature stress. Data based on chlorophyll fluorescence revealed a reduction of mean value of both cultivars for Fv/Fm, in late sown conditions. There were not any significant differences in all measured traits between two experimental years. Heat stress, occurred in late sown condition, caused reduction in biological and grain yield, harvest index, number of seed per ear, ear length and grain weight of two cultivars. But these reductions in durum wheat were lower. Seed protein content of cultivars increased in late sowing date. There were significant differences between cultivars in protein content. The grain yield reduction in cv. Roshan was higher than Dena. It seemed that by increasing in fructan and glucose content, rather than starch content, durum wheat can cope on the heat stress better than bread wheat.
In Poland, the genus Rubus comprises 95 species. Given the commercial production of the fruits as well as their medicinal properties and apicultural and ornamental importance, raspberries are commonly cultivated plants of great economic value. Rubi idaei folium exhibits a wide spectrum of pharmacological activities. The aim of the present study was to determine the content of some bioactive compounds and compare the antioxidant activity in R. ideaus leaves. The highest and lowest content of chlorophyll was found in the ‘Laszka’ and ‘Glen Ample’ varieties, respectively. The content of total fat, carbohydrate, and total protein was 2.1%, 6.2%, and 20%, respectively. Saturated fatty acids were dominated by palmitic, arachidonic, tetracosanoic, and stearic acids. Omega 3, omega 6, and omega 9 acids were mainly represented by α-linolenic acid, linolenic acid, and oleic acid, respectively. The highest antioxidant activity was determined in the leaves of the ‘Radziejowa’ variety, and the lowest level was found for the ‘Glen Ample’ variety. The high antioxidant activity and the content of bioactive compounds indicate that raspberry leaves can be used as drinkable infusions or extracts applied as additives to some food products.
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