Wyniki wyszukiwania - AGRO

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Effect of culture medium and illumination on the acid invertase activity in callus of Medicago strasseri

100%

Medina M., Villalobos N., De La Cruz P. J., Dorado A., Guerra H.

Acta Physiologiae Plantarum

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1999

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tom 21

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nr 2

The different acid invertase activity (total, soluble, wall-bound and extracellular) in calli induced on explants (cotyledon, petiole, hypocotyl and leaf) originated from Medicago strasseri seedlings were evaluated. In cultures subjected to 16 h photoperiod, the highest total, soluble and extracellular activities were found in calli from leaves cultured in medium 12 (MS with 0.01 mg·dm⁻³ (0.045 µM) of TDZ), elevated amounts of total and wall-bound invertase being found in calli induced on petioles in 12G medium (MS with 0.01 mg·dm⁻³ (0.045 µM) TDZ and 3.104 mg·dm⁻³ glycerol). In cultures maintained in darkness, the activity detected was lower than that observed in cultures under light conditions. The highest amounts of enzyme was bound in calli cultured on medium 12 (total and extracellular invertase) -leaves- and medium 12D (MS with 0.001 mg·dm⁻³ (0.0045 µM) TDZ) (soluble invertase) -using hypocotyls. In general, the different forms of invertase activity studied seem to appear in greatest amounts in calli induced under light conditions using leaves as explant and TDZ as growth regulator.

2

Cucurbita pepo L. var. patisoniana Greb. f. radiata Nois cultured in vitro. III. Influence of NAA and zeatin on cucurbitacins synthesis in Callus

86%

Nowak K., Rzepa J., Sajewicz M., Luczynska L.

Acta Biologica Silesiana

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1990

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tom 14

3

Somatic embryogenesis in Arabidopsis: Where do we go from here?

86%

Raghavan V.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

4

Regeneration of plantlets and tetraploidy induction in Pseudostellaria heterophylla

86%

Ye Z., Wang Y. Y., Tian H. Q.

Acta Biologica Cracoviensia. Series Botanica

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2009

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tom 51

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nr 2

13-18

This study was aimed at developing an efficient protocol for regeneration of Pseudostellaria heterophylla plantlets and induction of polyploidy. Calli of P. heterophylla (Miq) from stems, leaves and buds as explants could not differentiate into plantlets. However, young embryo segments used as primary explants produced embryonic calli on MS medium containing 5.0 mg/L 2,4-D and 0.5 mg/L KT. After the embryonic calli with granular protuberances were transferred to MS medium containing 0.5 mg/L BA, they developed shoots and then rooted to form plantlets. Polyploidy was induced when embryonic calli were placed in liquid MS medium containing 0.5% colchicine for 4 days, followed by culturing in solid medium to induce differentiation. Polyploidy was identified by the number of chromosomes and the size of plantlet stomata. The tetraploid plantlets produced larger root tubers than the diploid plantlets.

5

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Polyamine plays a role in subculture growth of in vitro callus of indica rice

86%

Acta Biologica Cracoviensia. Series Botanica

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2017

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tom 59

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nr 1

In vitro embryogenic callus is a critical factor for genetic transformation of rice, especially for indica varieties. In this study, we investigated the relationship between polyamines, including putrescine (Put), spermidine (Spd) and spermine (Spm), and callus browning, and we studied the effect of exogenous Put on callus regeneration and on the content of endogenous polyamines. In addition, the expression levels of arginine decarboxylase gene (Adc1) and S-adenosylmethionine decarboxylase gene (Samdc) in embryogenic callus were studied by quantitative Real-time PCR analysis. The results showed that the contents of endogenous Put and Spd in the browning callus were significantly lower than those in normal callus. Exogenous Put could effectively improve the growing state of callus of indica rice and enhance the development of embryogenic callus. The content of endogenous polyamines in embryogenic callus, especially Spd and Spm, was increased after addition of exogenous Put. Additionally, exogenous Put also had an obvious impact on the expression levels of Adc1 but partial effect on the expression levels of Samdc gene. This study could increase the knowledge of both embryogenic callus induction and polyamine catabolism in callus in indica rice.

6

In vitro plant regeneration of coffee Senna (Senna occidentalis) from hypocotyl-derived callus

86%

Isah T., Mujib A.

Acta Biologica Cracoviensia. Series Botanica

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2013

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tom 55

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nr 2

7

Indirect organogenesis of faba bean (Vicia faba L. minor)

86%

Skrzypek E., Czyczylo-Mysza I., Marcinska I.

Acta Biologica Cracoviensia. Series Botanica

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2012

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tom 54

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nr 2

Using four Polish Vicia faba L. minor cultivars (Bronto, Dino, Tibo, Nadwiślański) we obtained callus from epicotyl fragments collected from 7- and 14-day-old seedlings and from cotyledonary nodes of immature seeds. Callus induction efficiency varied from 81% to 97% depending on the origin of the explant. Shoots regenerated only from the cotyledonary nodes of all tested cultivars. On average, 50% of the explants grown on MS medium containing 1.0 mg dm-3 NAA, 0.5 mg dm-3 BAP, 0.25 mg dm-3 GA3, 1.0 g dm-3 casein hydrolysate, 750 mg dm-3 inositol, 3% sucrose and 0.4% agar were able to regenerate shoots. The number of calluses regenerating shoots was highest from explants collected from fruiting nodes 6 to 9. Decapitation of donor plants increased the percentage of calluses regenerating shoots. On half-strength MS medium with 2 mg dm-3 NAA and on 1/2 MS alone, 11% of the shoots rooted; on 1/2 MS with 1 g dm-3 AC, 8.0% rooted. The regenerants were transferred to Perlite with Hoagland medium and acclimated. Ten percent of the regenerated plants survived the acclimation process, flowered and produced seeds.

8

Somatic embryogenesis of Cyclamen persicum Mill.in seedlings culture

86%

Bach A., Malik M.

Acta Biologica Cracoviensia. Series Botanica et Zoologia. Supplement

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1997

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tom 39

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nr 1

9

The influence of phytohormones on the properties of wheat phospholipid monolayers at the water-air interface

86%

Filek M., Gzyl B., Dudek A.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 3

The surface behaviour of monolayers of wheat phospholipids in the presence of phytohormones introduced into the water phase was studied using Langmuir’s method. The phospholipids were extracted from the plasmalemma of non-embryogenic (NE) and embryogenic (E) calli initiated from two types of explant: immature inflorescences (inf) and embryos (emb). The surface properties were investigated in model systems of monolayers of mixed phospholipids with: 1) natural amphiphile composition (PL); 2) a determined hydrophobic part (16:0) and the natural percentage composition of the hydrophilic part (PPL); and 3) a determined hydrophilic part (PC) and the natural percentage composition of the hydrophobic part (HPL). The lower limit values of the molecular area (Alim) were observed for NE rather than for E monolayers in all the investigated systems (PL, PPL and HPL). The collapse pressure (πcoll) of the monolayer decreased in the order PPL>PL>HPL, indicating the high stability of monolayers containing saturated hydrocarbon chains. The injection of non-surface-active phytohormones into the water subphase and the subsequent formation of natural and also artificial phospholipid monolayers of E and NE causes a decrease in monolayer stability against collapse and molecular close packing. As a result of their amphipathic (hydrophilic-hydrophobic) structure, the surface properties of E phospholipids are probably optimal for these systems. The decreasing stability of the NE monolayer caused by the presence of the phytohormone seems to be advantageous in terms of membrane preparation for the differentiation process. All the investigated lipid monolayers (highly) stimulated the adsorption of indole-3-acetic acid (to the highest extent/degree) (among the examined phytohormones) from the subphase. Zearalenone had a significant influence on the surface properties of NE PPL and NE HPL monolayers. This may be connected with the ability of this phytohormone to affect the non-embryogenic structure of wheat. An anomalous temperature effect was observed in the presence of indole-3-acetic acid (IAA) in the bulk; phospholipid monolayers of embryogenic calli induced from embryos (E emb) when the temperature decreased from 25 to 15°C. This phenomenon is ascribed to the dehydration of the polar groups in the monolayer.

10

Morphogenesis of tomato callus cultures infected with the cucumber mosaic virus [CMV]

86%

Hanus-Fajerska E.

Acta Physiologiae Plantarum

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1997

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tom 19

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nr 2

11

Growth characteristic and ion contents of rice callus under the influence of NaCl and hydroxyproline

86%

Chauhan V., Prathapasenan G.

Acta Physiologiae Plantarum

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2000

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tom 22

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nr 1

12

86%

Anjum M. A.

Acta Physiologiae Plantarum

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1998

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tom 20

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nr 2

Freshly isolated mesophyll and suspensions-cell protoplasts of S. tuberosum cvs. Desiree and Maris Piper were cultured in different media i.e. modified MS, V-KM and MS-KM. Protoplast plating efficiencies were higher in MS-KM medium. Resulting protoplast-derived calluses were transferred either onto the medium of Bokelmann and Roest (1983) or that of Lam (1977) for shoot regeneration. Calluses derived from mesophyll cell protoplasts differentiated about 2 weeks earlier than calluses derived from suspension-cell protoplasts. Shoot initiation was also about 2 weeks earlier from calluses subcultured onto the former medium as compared to the latter.

18

Transfer the readiness to earing of regenerants obtained via callus from some genotypes of winter triticale

86%

Marcinska I., Kaminski M., Biesaga-Koscielniak J., Dubert F.

Acta Physiologiae Plantarum

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1997

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tom 19

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nr 2

19

Effect of culture medium and light conditions on the morphological characteristics and carbohydrate contents of Medicago strasseri calli

86%

Medina M., Villalobos N., De La Cruz P. J., Dorado A., Guerra H.

Acta Physiologiae Plantarum

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1998

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tom 20

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nr 4

Using 6 culture media (12, 12D, 12G, 11, A and B) made up of MS medium (Murashige-Skoog, 1962) supplemented or not with glycerine, with different cytokinins, and/or 2,4-D, the morphological characteristics and contents in total carbohydrates, reducing sugars, sucrose and starch were studied in calli induced from explants (cotyledon, petiole, hypocotyl and leaf) obtained from Medicago strasseri seedlings. Callus formation was induced under photoperiod (16h light/8h darkness) conditions or in the absence of light. Considerable variability in the calli was observed, depending on the explants and media used. Under photoperiod conditions, medium A with KIN (1 mg/l) and 2,4-D (3 mg/l) induced many calli with the highest contents in total carbohydrates (886.1–889.3 mg/g DW), sucrose (132.1–188.2 mg/g DW) and starch (125.2–247.6 mg/g DW) and the lowest contents in reducing sugars (118.4–173.3 mg/g DW). In media 11, A and B, under conditions of darkness, calli degenerated at the start of culture. Calli developed in darkness generally had dry weights and total carbohydrate and starch contents lower than those cultured under photoperiod conditions. However, sucrose contents were greater in calli formed in darkness. At these cultures times, differentiation, in the form of organogenesis, was only seen using medium B with cotyledons, petioles and leaves as explants. It was also observed when petioles were cultured in medium A but with a less pronounced organogenic response.

20

A simple and efficient method for cryopreservation of embryogenic triticale calli

86%

Sowa S., Oleszczuk S., Zimny J.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 2

Conventional breeding methods are now supplemented by modern in vitro techniques. However, long term maintenance of cultures has many disadvantages. It incurs risk of loss through microbial contamination, somaclonal variation or human error, but above all the regeneration capacity can decrease gradually during extended maintenance. Cryopreservation as a method of long term storage of biological material without genetic alteration was adapted for embryogenic triticale calli preservation. Callus of both winter and spring genotypes were successfully cryopreserved. The best viability rates (80-85 %) were achieved with 6 weeks old winter genotypes treated with cryoprotective solution containing DMSO. This simple and efficient method of cryopreservation requires no special devices for controlled freezing and can be easily adapted for other cereals.

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