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The level of IAA and ABA in lateral buds of birch shoots 24 h and 5 days after the decapitation of the apical bud was determined. Twenty four hours after decapitation, when visible signs of outgrowth of lateral buds were not observed yet, an increase in the level of IAA and a decrease of ABA, as compared with the buds of non-decapitated shoots, was found. Five days later, when lateral buds were in the period of intensive outgrowth, a decrease in the levels of IAA and ABA was observed. It has been suggested that removing the source of auxin, by the decapitation of the apical bud makes possible the lateral buds to undertake the synthesis of their own auxin. It could lead to the decrease in the content of ABA. These all events could create suitable conditions for the outgrowth of lateral shoots.
Stigmas isolated from flower buds of ‘Nakielska’ variety of Sinapis alba were used to develop a micropropagation method suitable for breeding of new cultivars. The origin of adventitious bud regeneration was studied on MS medium, under stimulation by bezylaminopurine (BAP) in combination with 2,4-D - naphtoxy acetic acid (2,4-D). Histological analysis showed the structure of Sinapis stigma (composed from four types of tissue: papillae, transmitting tissue, parenchyma and vascular bundles) and revealed that numerous meristematic centers developed from parenchyma cells in close vicinity of vascular bundles. Buds very quickly appeared on the surface of initial explants and later formed multiplantlets that were easily rooted in the soil.
Since the contribution of total belowground bud bank and different bud types to community regeneration has rarely been explored, the vegetative offspring recruited from different belowground bud types was investigated in four plant communities along a grassland degradation gradient in northeastern China (Inner Mongolia). This gradient, between 1000 and 1500 m a.s.l., has been caused by overgrazing. It is a Leymus chinensis steppe which occupies about 3.0×10⁵ ha. Recruitment from tiller buds was dominant (>80%) in determining the total vegetative offspring density along the whole grassland degradation gradient. However, the proportional contribution of tiller-ramets to total ramet recruitment was significantly greater (P <0.05) during earlier than later stages of grassland degradation, while that of rhizome-ramets showed an opposite pattern. While the percentage contribution and density of root-derived ramets to total ramet density increased significantly (P <0.05) during the late stages of grassland degradation, those of bulb-ramets kept relatively constant along the whole grassland degradation gradient. The relative contribution of hemicryptophytes [i.e., Achnatherum sibiricum, Cleistogenes squarrosa, Festuca ovina, Koeoleria cristata, Poa annua, Stipa grandis] to total plant species richness decreased, while that of geophytes [i.e., Agropyron cristatum, Carex korshinskyi. Leymus chinensis, Allium anisopodium, A. bidentatum, A. tenuissimum, Astragalus galactites, Cymbaria dahurica, Iris tenuifolin, Potentilla acaulis, P. bifurca, Pulsatilla turczaninovii, Serratula chinensis, Thalictrum aquilegifolium] increased with the increases of grassland degradation. Our results showed that as grassland degradation increased, changes in the proportion of tiller-, rhizome- and root-derived ramets with respect to total ramet density determined in turn changes in the proportion of hemicryptophytes and geophytes in the study plant communities.
The presence of phytoplasmas in Lilium sp. showing severely stunted growth, leaf malformation and flower buds deficiency was demonstrated for the first time using polymerase chain reaction assays with primers amplifying phytoplasma 16S rDNA regions. These phytoplasmas were found in leaves as well as roots and bulb scales of symptomatic and CMV and/or LSV affected and asymptomatic virus-free lilies.
In vitro organogenesis in hypocotyl explants of the pepper cultivar ‘Bryza’ was induced on MS medium containing 5 mg/l 6-benzyloaminopurine (BAP) and 1 mg/l indole-3-acetic acid (IAA). The hypocotyl explants were then inoculated with Agrobacterium tumefaciens LBA4404(pBI121). After 2 days of culture the first cell divisions were observed in the epidermis and cortex. After 6-7 days, numerous adventitious bud primordia appeared in 58.4% of the explants. In further stages of culture, buds developed into shoots in 8.4% of the explants. Histological analysis revealed hypertrophy and the presence of necrotic cells in the cortex. Necrotic changes were also observed in the vascular bundles. It is likely that culture on a selective medium containing kanamycin and co-culture with Agrobacterium tumefaciens strongly affected the organization of the hypocotyl meristematic tissue, and in consequence brought about necrosis and isolation of the adventitious buds from the vascular bundles.
This study assessed regrowth of micropropagated Rhodiola Kirilowii buds after encapsulation of axillary buds and differentiating callus in calcium alginate hydrogel and low-temperature preservation. This method of micropropagation was applied to obtain enough plant material for studies on chemical compounds of Rhodiola Kirilowii, a plant difficult to obtain from its natural environment. Axillary buds and differentiating callus were encapsulated in calcium alginate and stored at 4°C for 1 to 15 weeks and then transferred to hormone-free MS medium. The best results were obtained after six weeks of preservation: 100% of the encapsulated explants survived and developed into shoots and plantlets after subculture on basal MS medium. Longer storage of encapsulated axillary shoot buds decreased their regrowth, but the duration of storage of encapsulated differentiating callus had no significant influence on its survival.
This work is the first characterization at cellular and subcellular level of the main cellular events occurring in the first stages of microspore embryogenesis. Microspore embryogenesis was induced in two varieties of Citrus Clementina (Nules and Monreal). The results showed that one of the most responsive stages for embryogenesis was the vacuolate microspore. Microscopic analysis revealed specific features of the young proembryos still surrounded by the exine: large nuclei, clear areas in the cytoplasm, starch accumulation, and an increase in the thickness of the wall under the exine. Immunogold labelling with JIM 5 antibody showed a high amount of non-esterified pectins in the surrounding cell wall. After exine rupture, different cell types were detected in late proembryos. As embryogenesis proceeded, the normal pattern of development was observed, including heart-shape, torpedo and cotyledonar embryos.
This experimental study was conducted at the Fruit Research Institute, Cacak, Serbia, to determine bud break and callus formation dates, the degree of callus forma­tion, grafting success on day 20 after grafting and overall grafting success on day 28 after grafting in walnut (Juglans regia L.) under different treatments and bench graft­ing conditions in cultivar Seinovo (control) and the selections 'Ovcar', 'G-286', 'Elit' and 'G-139'. Three treatments were applied: Treatment 1 - covering the graft with sawdust up to the top of the scion without dipping the scion and graft union in paraf­fin; Treatment 2 - covering the graft with sawdust up to the top of the scion and dip­ping the scion and graft union in paraffin; and Treatment 3 - covering the graft with both sawdust, up to the top of the scion, and polyethylene foil, and dipping the scion and graft union in paraffin. The best results of the parameters tested were obtained in the treatment involving dipping the graft in paraffin and covering it with both saw­dust, up to the scion, and polyethylene foil. The treatment without the use of paraffin, involving only the covering with conifer sawdust up to the top of the scion yielded the poorest results.
Following the winter of 2008/2009, an assessment of frost damage was carried out on the vines of forty-one wine and twenty-five table grape cultivars grown in central Poland. The minimum winter temperature of-23°C was recorded on 6 January 2009. The best tolerance to low temperatures (no damage to shoots or buds) was shown by the interspecific hybrids 'Price', 'Supaga' and 'Zilga' of the Vitis labrus- cana group and hybrid 'Baco Noir' originated from V. vinifera and V. riparia. Frost injuries of the other grapevines classed as interspecific hybrids varied from 0.7% of frozen buds ('Aurore' and 'Refren' - hybrids of V. vinifera, V. rupestris, and V. lincecumii) to 99.5% ('Kodrianka'). 'Riesling' (13.5% of frozen buds) showed the best tolerance to frost damage from all the V. vinifera cultivars. The buds of the V. vinifera cultivar 'Favorit' all froze. Plant parts which were covered with soil and snow were not exposed to significant damage, even in susceptible cultivars.
The primary goals of this study were to quantify the composition and size of bud banks and to evaluate the roles of bud banks of main dominant species Leymus chinensis (Trin.) Tzvel. and Carex duriuscula C.A. Mey. in population maintenance over a 2-year period. The four experimental plots were in early, early-mid, middle and late seral stages of a flooded restoration succession after they had approximately 12, 10, 8, and 6 months’ flood durations in the Songnen meadow, China. Five quadrats (each 0.25 ´ 0.25 m in area and 30 cm deep) were each sampled in four seral spots. The differences of L. chinensis and similarity of C. duriuscula in bud bank characteristics persisted in the studied seral stages from year to year. In each seral stage, rhizome buds comprised most of the L. chinensis bud banks in 2003; in 2004, juvenile tillers predominated. Rhizome buds consisted of the majority of C. duriuscula bud banks throughout the study period. The bud densities of L. chinensis and C. duriuscula experienced a significant linear increase with the increase of tiller density at different seral stages each year, but the slopes of these relationships in L. chinensis were higher than those in C. duriuscula. The total bud densities of C. duriuscula showed a significant linear decrease corresponding to the increase of those of L. chinensis from the early to the late seral stage in 2003 and 2004. Bud banks are vital for population maintenance and the number of bud banks is a good predictor of population dynamics.
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