Samples of orange juices were evaluated according to standard criteria in Code of Practice and compared with standard RSK values (Richtwerte und Schwankungsbreiten bestimmter Kennzahlen). In 15 orange juices, the content of chlorides, total acidity, volatile acids and ammonia did not exceed values in Code of Practice. It was found that samples F and K (66.30 g/dm3 and 57.65 g/dm3) contained more glucose than is allowed by Code of Practice. Juices G and K did not exceed RSK values for the D-isocitric acid content (103.68 g/dm3 and 109.96 g/dm3). Criterion according to citric and D-isocitric acid ratio corresponded to the normal values in 100% orange juice in samples G, K, L and also in 12% orange nectar P (69.3; 118.6; 51.4 and 72.3).
Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis was used to identify fish species and to perform their authentication in commercial seafood products. Universal primers were used for PCR amplification of 464-bp long fragments of the mitochondrial cytochrome b gene. The PCR products were digested with restriction enzymes AluI, HinfI, HaeIII, DdeI, NlaIII, HincII, and MboII. Sixty fish samples were obtained from the local markets in the Czech Republic. In 47 samples (78.3%), the results were in agreement with declarations of the producers and 10 samples (16.7%) contained other fish species. There were not great differences between fresh fillets (chilled, frozen etc.) and heat-processed foodstuffs (tinned, smoked, etc). The correct designation was in 72.3% and 81.6% of samples, respectively. Even if in three cases the analysis was unsuccessful the method is useful for the control of the adulteration of food with fish tissue content and in this way it contributes to better consumer awareness.
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