Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 18

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  antifungal agent
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Lipid-based antifungal agents

100%
Arikan S.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr 2
2

Emodin reduces the activity of (1,3)-beta-D-glucan synthase from Candida albicans and does not interact with caspofungin

75%
Janeczko M.
Polish Journal of Microbiology
|
2018
|
tom 67
|
nr 4
Candidiasis is the most common opportunistic yeast infection, with Candida albicans as a paramount causative species. (1,3)-β-D-glucan is one of the three main targets of clinically available antifungal agents used to treat Candida infections. It is one of the most abundant fungal cell wall components. Echinocandins represent the newest class of antifungals affecting cell wall biosynthesis through non-competitive inhibition of (1,3)-β-D-glucan synthase. Therefore, treatment with echinocandins causes defects in fungal cell integrity. In the present study, similar activity of emodin (6-methyl-1,3,8-trihydroxyanthraquinone) has been revealed. Many reports have already shown the antifungal potential of this pleiotropic molecule, including its activity against C. albicans. The aim of this report was to evaluate the activity of emodin towards a new molecular target, i.e. (1,3)-β-D-glucan synthase isolated from Candida cells. Moreover, given the identical mechanism of the activity of both molecules, interaction of emodin with caspofungin was determined. The study revealed that emodin reduced (1,3)-β-D-glucan synthase activity and increased cell wall damage, which was evidenced by both a sorbitol protection assay and an aniline blue staining assay. Furthermore, the synergy testing method showed mainly independence of the action of both tested antifungal agents, i.e. emodin and caspofungin used in combination.
3

Recent progress in antifungal agents

75%
Gozzo F.
Pestycydy
|
1990
|
nr 1
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Antifungal activity of essential oils on Aspergillus parasiticus isolated from peanuts

75%
Yooussef M. M., Pham Q., Achar P. N., Sreenivasa M. Y.
Journal of Plant Protection Research
|
2016
|
tom 56
|
nr 2
Aspergillus parasiticus is one of the most common fungi which contaminates peanuts by destroying peanut shells before they are harvested and the fungus produces aflatoxins. The aim of this study was to evaluate the antifungal activities of seventeen essential oils on the growth of the aflatoxigenic form of A. parasiticus in contaminated peanuts from commercial outlets in Georgia. The agar dilution method was used to test the antifungal activity of essential oils against this form of A. parasiticus at various concentrations: 500; 1,000; 1,500; 2,000; 2,500 ppm. Among the seventeen essential oils tested, the antifungal effect of cinnamon, lemongrass, clove and thyme resulted in complete inhibition of mycelial growth. Cinnamon oil inhibited mycelial growth at ≥ 1,000 ppm, lemongrass and clove oils at ≥ 1,500 ppm and thyme at 2,500 ppm. However, cedar wood, citronella, cumin and peppermint oils showed partial inhibition of mycelial growth. Eucalyptus oil, on the other hand, had less antifungal properties against growth of A. parasiticus, irrespective of its concentration. Our results indicate that the aflatoxigenic form of A. parasiticus is sensitive to selected essential oils, especially cinnamon. These findings clearly indicate that essential oils may find a practical application in controlling the growth of A. parasiticus in stored peanuts.
5

Lipid-based antifungal agents: a concise overview

75%
Arikan S.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr 3
The development of lipid formulations of antifungal drugs has been a remarkable progress in the systemic antifungal arena. The lipid-based amphotericin B formulations; amphotericin B lipid complex (ABLC) amphotericin B colloidal dispersion (ABCD), and 1iposomal amphotericin B (L-AMB) have been in clinical use since the 1990s. They are significantly less nephrotoxic than the parent compound and can be safely used at higher doses. The primary cost of these formulations is significantly high and the extent of data related to their head-to-head comparison remains limited. The lipid formulation of nystatin, liposomal nystatin, is another lipid-based polyene under development. Available data concerning the in vitro activity, pharmacokinetic profile, in vivo efficacy, and safety of these formulations are summarized in this overview.
6

Ergosterol biosynthesis inhibition: a target for antifungal agents

75%
Barrett-Bee K., Dixon G.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 4
The isoprenoid sterols play a crucial role in the viability of all fungi; those unable to synthesise ergosterol because of inhibition, growth conditions or mutation must take it up from the environment. A range of compound types have been discovered which interfere with the biosynthetic pathway from acetate to ergosterol and these compounds have antifungal actions. Inhibition of several of the steps has yielded agents which have been used with great success as medical and agrochemical agents. The most important biosynthetic steps that have been exploited are inhibition of squalene epoxidase, (the allylamines and tolnaftate) C14 demethylation (the azoles), A7,8 isomerase and A14 reductase which are inhibited by the morpholines. Recent research has shown that inhibition of C24 methyltransferase and C4 demethylation also yield antifungal agents. Combination studies demonstrate that synergy between agents of different types can be measured. Fungicidal effects were observed when a combination of two fungistatic agents was used.
7

Glucosamine-6-phosphate synthase, a novel target for antifungal agents. Molecular modelling studies in drug design

75%
Wojciechowski M., Milewski S., Mazerski J., Borowski E.
Acta Biochimica Polonica
|
2005
|
tom 52
|
nr 3
Fungal infections are a growing problem in contemporary medicine, yet only a few antifungal agents are used in clinical practice. In our laboratory we proposed the enzyme L-glutamine: D-fructose-6-phosphate amidotransferase (EC 2.6.1.16) as a new target for antifungals. The structure of this enzyme consists of two domains, N-terminal and C-terminal ones, catalysing glutamine hydrolysis and sugar-phosphate isomerisation, respectively. In our laboratory a series of potent selective inhibitors of GlcN-6-P synthase have been designed and synthesised. One group of these compounds, including the most studied N3-(4-methoxyfumaroyl)-l-2,3-diaminopropanoic acid (FMDP), behave like glutamine analogs acting as active-site-directed inactivators, blocking the N-terminal, glutamine-binding domain of the enzyme. The second group of GlcN-6-P synthase inhibitors mimic the transition state of the reaction taking place in the C-terminal sugar isomerising domain. Surprisingly, in spite of the fact that glutamine is the source of nitrogen for a number of enzymes it turned out that the glutamine analogue FMDP and its derivatives are selective against GlcN-6-P synthase and they do not block other enzymes, even belonging to the same family of glutamine amidotransferases. Our molecular modelling studies of this phenomenon revealed that even within the family of related enzymes substantial differences may exist in the geometry of the active site. In the case of the glutamine amidotransferase family the glutamine binding site of GlcN-6-P synthase fits a different region of the glutamine conformational space than other amidotransferases. Detailed analysis of the interaction pattern for the best known, so far, inhibitor of the sugar isomerising domain, namely 2-amino-2-deoxy-d-glucitol-6-phosphate (ADGP), allowed us to suggest changes in the structure of the inhibitor that should improve the interaction pattern. The novel ligand was designed and synthesised. Biological experiments confirmed our predictions. The new compound named ADMP is a much better inhibitor of glucosamine-6-phosphate synthase than ADGP.
8

Inhibition of growth and sporulation of Penicillium expansum by extracts of selected Basidiomycetes

75%
Florianowicz T.
Acta Societatis Botanicorum Poloniae
|
2000
|
tom 69
|
nr 4
9

Brefeldin A decreases the activity of the general amino acid permease [GAP1] and the more specific systems for L-leucine uptake in Saccharomyces cerevisiae

75%
Alonso M., Burgos H. I., Pannunzio V., Hughes A. M., Mattoon J. R., Stella C. A.
Cellular and Molecular Biology Letters
|
2006
|
tom 11
|
nr 2
Brefeldin A is a commonly used antifungal agent that reversibly blocks protein transport from the endoplasmic reticulum to the Golgi complex. In this study, we aimed to characterize L-leucine uptake in Saccharomyces cerevisiae in the presence of brefeldin A. For this purpose, we used a synthetic medium, containing L-proline and the detergent SDS, which allows the agent to permeate into the yeast cell. The results obtained with a wild type strain and a gap1 mutant indicate that BFA causes either direct or indirect modification of the transport and/or processing of L-leucine permeases. The presence of BFA affects the kinetic parameter values for L-leucine uptake and decreases not only the uptake mediated by the general system (GAP1), but also that through the specific BAP2 (S1) and/or S2 systems.
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Structure-activity relationship study for fungicidal activity of 1-(4-phenoxymethyl-2-phenyl-[1,3]dioxolan-2-ylmethyl)-1H-1,2,4-triazole derivatives against rice blast

63%
Hoshi T., Yamada K., Yoshizawa Y., Oh K.
Journal of Plant Protection Research
|
2015
|
tom 55
|
nr 4
To explore new antifungal agents for rice blast control, the antifungal activity of a series of novel 1,2,4-triazole derivatives against Magnaporthe oryzae has been evaluated. The antifungal activity was determined by using in vitro mycelial growth inhibition tests. Among the 19 test compounds, we found that the compound 1-(4-phenoxymethyl-2-phenyl-[1,3]dioxolan-2-ylmethyl)-1H-1,2,4- triazole (Gj) displayed potent antifungal activity against M. oryzae. The IC50 value was found approximately 3.8±0.5 μM and the IC50 value of propiconazole was found to be approximately 3.7±0.2 μM, respectively. Structure-activity relationship studies on aromatic ring structures provided insight and information about the structural requirements for antifungal activity of this synthetic series against M. oryzae.
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Fenton reagent and titanium dioxide nanoparticles as antifungal agents to control leaf spot of sugar beet under field conditions

63%
Hamza A., El-Mogazy S., Derbalah A.
Journal of Plant Protection Research
|
2016
|
tom 56
|
nr 3
In this study, foliar sprays of Fenton solutions (Fenton reaction, Fenton-like reaction and Fenton complex), titanium dioxide (TiO2) and the recommended fungicide (chlorothalonil) were estimated in the control of sugar beet leaf spot caused by Cercospora beticola under field conditions in two growing seasons. In addition, the impacts of these treatments on some crop characters (leaf dry weight, root fresh weight, soluble solid content, sucrose content and purity of sugar) were examined. Biochemical and histological changes in the livers and kidneys of treated rats compared to an untreated control were utilized to assess the toxicity of the examined curative agents. Overall, chlorothalonil and Fenton complex were the most effective treatments for disease suppression in both tested seasons followed by Fenton-like reagent, Fenton’s reagent and TiO2, respectively. Growth and yield characters of treated sugar beet significantly increased in comparison to an untreated control. There were mild or no (biochemical and histological) changes in the livers and kidneys of treated rats compared to the control. Fenton solutions and TiO2 may offer a new alternative for leaf spot control in sugar beet.
12
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Treatment of seborrheic dermatitis: The efficiency of sertaconazole 2% cream vs. tacrolimus 0.03% cream

63%
Goldust M., Rezaee E., Raghifar R., Hemayat S.
Annals of Parasitology
|
2013
|
tom 59
|
nr 2
The treatment of seborrheic dermatitis (SD) includes topical antifungal agents to eradicate Malassezia spp. corticosteroids to treat the inflammatory component of the disease, and keratolytics to remove scale and crust. The aim of this study was to compare the efficiency of sertaconazole 2% cream and tacrolimus 0.03% cream in the treatment of seborrheic dermatitis. In this clinical trial study, sixty patients suffering from SD were studied. Thirty patients received local sertaconazole 2% cream with a recommendation to use the cream twice a day for 4 weeks. In the control group, thirty patients received tacrolimus 0.03% cream twice a day for four weeks. At the time of referral, and 2 and 4 weeks after first visit, the patients were examined by a dermatologist to check the improvement of clinical symptoms. The mean ages of the sertaconazole and tacrolimus groups were 30.98 ± 12.24 and 34.67 ± 10.82, respectively. The highest level of satisfaction (90%) was observed 28 days after sertaconazole use. Only 83.3% satisfaction was noted in the tacrolimus group. The relationship between patient satisfaction and sertaconazole 2% cream receive in 28th day was significant (P=0.006). Sertaconazole 2% cream may be an excellent alternative therapeutic modality for treating seborrheic dermatitis.
13
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Biologically active plant-derived substances with antimicrobial properties

63%
Tokarska Rodak M.
Health Problems of Civilization
|
2021
|
tom 15
|
nr 2
Modern medicine, as well as the pharmaceutical, cosmetic and food industries appreciate the value of plant raw materials for their antiseptic, analgesic, anti-inflammatory and soothing effects. Medicinal plants, often found endemically, are often treated as potential sources of new substances with antimicrobial and antifungal activity. The aim of this study was to analyze the significance of selected medicinal and spice plants in terms of their ability to inhibit the growth of microorganisms, including those pathogenic to humans. The significance of active substances contained in selected plant species with high antimicrobial potential including Aloe vera L. and Chelidonium majus L. was described. The antimicrobial activity of plant oils, including those extracted from citrus and spice plants, was also analyzed. Plants are a rich source of many substances with a broad spectrum of activity. This knowledge has often been used in folk medicine, but it was not until the development of modern research methods that the mechanisms underlying the therapeutic properties of many plant substances and their proper use could be understood. New substances present in plant materials are constantly being discovered that may be of medical interest because of their great potential to be used against microorganisms or because of their antioxidant and anti-cancer properties.
14
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Biomass, abundance and sensitivity to antibiotics and antimycotics of the fungi in the Vistula River with its main tributaries

63%
Pietryczuk A., Gorniak A. S., Wiecko A., Cudowski A.
Acta Mycologica
|
2013
|
tom 48
|
nr 2
Mycoplankton of Vistula River and its main tributaries biomass as well as the number and morphotype diversity was studied in summer and autumn 2011. Summer mycoplankton biomass was within the range of 0.2 – 0.5 μg/l, while in the autumn it was two times wider range (0.1 – 1.3 μg/l). The number of fungi in river water most often did not exceed 1000 – 2000 CFU/ml. Fungi colonies isolated from rivers water were sensitive to the commonly used amphotericine B (10 μg) and gentamicin (10 μg). It seems to be plausible that aquatic fungi can acquire immunity to drugs as a result of horizontal transfer of a gene responsible for drug resistance or as an effect of antibiotics and antimycotics getting into the aquatic ecosystems from wastewaters.
15

Fungal glycoproteins and their biosynthetic pathway as potential targets for antifungal agents

63%
Tanner W., Gentzsch M., Immervoll T., Scheinost A., Strahl-Bolsinger S.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 4
The yeast cell wall as a good antifungal target is discussed in general. More specifically the reaction, catalyzed by Dol-P-Man: protein O-D-mannosyltransferase is proposed as a new potential target. Six genes responsible for this endoplasmic reticulum-localized reaction have been cloned and characterized so far. Triple dis­ruptions of these genes are either lethal or the corresponding cells have to be osmotically stabilized to survive. No inhibitors of this reaction are as yet known.
16

Targeting the fungal plasma membrane proton pump

63%
Monk B. C., Mason A. B., Kardos T. B., Perlin D. S.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 4
The need for new mechanistic classes of broad spectrum antifungal agents has prompted development of the membrane sector and ectodomain of the plasma membrane proton pumping ATPase as an antifungal target. The fungal proton pump is a highly abundant, essential enzyme in Saccharomyces cerevisiae. It belongs to the family of P-type ATPases, a class of enzymes that includes the Na+,K+-ATPase and the gastric H+,K+-ATPase. These enzymes are cell surface therapeutic targets for the cardiac glycosides and several anti-ulcer drugs, respectively. The effects of acid- -activated omeprazole show that extensive inhibition of the S. cerevisiae ATPase is fungicidal. Fungal proton pumps possess elements within their transmembrane loops that distinguish them from other P-type ATPases. These loops, such as the conformationally sensitive transmembrane loop 1+2, can attenuate the activity of the enzyme. Expression in S. cerevisiae of fully functional chimeric ATPases that contain a foreign target comprising transmembrane loops 1+2 and/or 3+4 from the fungal pathogen Candida albicans suggests that these loops operate as a domain. The chimera containing C. albicans transmembrane loops 1+2 and 3+4 provides a prototype for mutational analysis of the target region and the screening of inhibitors directed against opportunistic fungal pathogens. Panels of mutants with modified ATPase regulation or with altered cell surface cysteine residues are also described. Information about the ATPase membrane sector and ectodomain has been integrated into a model of this region.
17

Antifungal susceptibility of yeasts isolated from secrection of inflamed mammary glands in cows

51%
Wawron W., Bochniarz M., Dabrowski R.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 3
18
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Antimicrobial activity of chlorophyll and methanol extract of Lannea coromandelica Merr.

38%
Vedhanarayanan P., Vaithiyanathan T., Sundaramoorthy P.
International Letters of Natural Sciences
|
2015
|
tom 45
The antimicrobial activity of chloroform and methanol extracts of Lennea coromandelica were screened for their was studied against gram positive bacteria strains Staphylococcus aureus, Streptococcus pyogenes and Bacillus subtilis gram negative bacteria strains Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli and fungal strains such as Candida albicans, C. parapsilosis, C. krusei and C. glabrata using disc diffusion method, determination of Minimum Inhibitory Concentrations (MIC), Minimum Bacterial Concentrations (MBC) and Minimum Fungicidal Concentrations (MFC). The antimicrobial activity for different concentrations like 250 μg, 500 μg and 1000 μg of different solvent extracts of Lannea cormandelica. bacterial strains and recorded in highest mean zones of inhibition ranged from 19.6 mm and Candidal strains and the exhibited the highest mean zones of inhibition ranged from 10.6 mm. Methanol extracts showed the best results as inhibition zones against tested organisms. Results showed also that, the greatest effect was towards Staphylococcus aureus and the lowest was against Candida krusei. The present study reported the great effect of Lannea cormandelica extracts against some of most important pathogens.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.