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The paper presents a comparative analysis of wood calorific value of grand fir (Abies grandis Lindl.), originating from four stands of the southern Poland. The research material were the samples, collected from the trunks of 30 trees in each stand. The samples were divided into sections, each containing five annual increments. Then each section was measured: annual ring width and the relative wood density, which was converted to dry wood density, taking the total volumetric shrinkage of 11%. It was assumed that the calorific value of 1 kg dry wood of grand fir is 20.15 MJ. On the basis on the analysis it was found that the average calorific value of 1 m3 of grand fir wood was 8021 MJ. It was shown, that a significantly higher calorific value was obtained by the combustion of 1 m3 wood of giant fir trees, growing on the poorer soil in comparison to the more fertile soils. Statistical analysis also showed that with increasing age of the trees the calorific value of 1 m3 grand fir wood increases significantly.
Ectomycorrhizal and saprobic fungi of two industrial wastes in southern Poland (calamine spoil in Bolesław and zinc waste in Chrzanów) were studied. Pine (Pinus sylvestris) accompanied by birch (Betula pendula) were present in the investigated area. Fruitbodies of 68 species were recorded, but only 10 were common to both sites. Mycorrhizal species were the most common group on the zinc waste, whereas saprobic fungi prevailed on the calamine spoil. The differences in species composition between sites might be due to differences in plant cover, but also to the toxicity of the material at the sites. Among mycorrhizal species, members of Cortinariaceae and Tricholomataceae were most frequently recorded. Most ectomycorrhizal species had a broad host range, and only a few species known to be associated exclusively with pine or birch were found. Analysis of ectomycorrhizas by classical and molecular (PCR-RFLP) methods revealed that the fungi forming the most abundant fruitbodies were also present in the form of ectomycorrhizas. A few ascomycete and basidiomycete fungi not recorded as fruitbodies were present as pine symbionts.
Niepołomice Forest is located about 20 kilometers east of Cracow (Małopolska province, southern Poland). Its natural and touristic values, as well as wide range of hosts occurring within indicate this to be an area of high risk of exposure to Ixodes ricinus and tick-borne diseases it transfers. I. ricinus is a common species in Poland and Europe. Its seasonal activity begins in Poland in the early spring, and ends with late autumn. A total number of 129 specimens of I. ricinus was collected by flagging in Niepołomice Forest. DNA was isolated by ammonia method from 30 randomly-selected individuals. PCR was used to detect tick-borne pathogens with primers specific for Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato and Babesia sp. Molecular studies confirmed the presence of all three pathogens in I. ricinus. A. phagocytophilum was found in 76.7%, Babesia sp., 60%, B. burgdorferi s. l., in 3.3% of studied ticks. A. phagocytophilum co-infection with Babesia sp., was found in 46.7% of the specimens. A co-infection of all three tested pathogens was recorded in one case (3.3%). In Poland the problem of tick-borne diseases is a growing issue, therefore people residing in southern Polish touristic areas should be informed about the prevention and protection against ticks.
The aim of the study was to analyse the grass pollen season dynamics in Cracow in 1991-2008 and to find the relationship between the meteorological conditions and season parameters. The pollen season started on average on the 138th day of the year (±10 days) and ended on the 211th day (±10 days). The median of the SPI value (calculated by the 95/90% method) was 2041, peak concentration – 111 pgm-3 (achieved on average on the 34th day of the season/173th day of the year). The average temperature in April-May showed the greatest effect on the start and end of the season (negative correlation). Tmean of the third decade of June influenced the season end (rs negative). Tmean of April-May correlated negatively with the time of peak concentration. Rainfall in January and April influenced the season start. Relative sunshine in March-April was related to the season start, in the third decade of June it was related to the season end (negative correlation), in March-April it influenced the time of peak concentration (positive correlation), while in January-March the peak concentration (positively). Cloudiness in February-May and also in the third decade of June had an impact on the season end and time of peak concentration (positive correlation). Humidity in February influenced the season start (negatively). The SPI value was influenced by relative sunshine (positively) in February-May, by humidity in April, and cloudiness in February-March (negatively).
One hundred and one stands of non-native red oak Quercus rubra L. were examined across various forest types with the objective to relate the influence of environmental variables on the abundance of this species and to characterise its impact on the species richness and proportion of functional groups of native plant species. Amongst randomly selected stands more than 50% were self-regenerating ones, i.e. seedlings were present. The cover of Q. rubra seedlings was positively correlated with light intensity (rs = 0.38, P <0.05) and negatively with the content of nitrogen (rs = –0.20, P <0.05) and loss on ignition (rs = 0.20, P <0.05) in soil. The cover of mature trees was positively correlated with the value of pH (rs = 0.22, P <0.05). Separate correlations with DCA scores of phytosociological relevés and the cover of red oak in each layer showed that the species influenced the floristic composition of forest vegetation. The cover of mature trees of red oak negatively affected both the number of understorey species (rs = –0.39, P <0.01) and the cover of shrubs (rs = –0.21, P <0.05) and herbaceous species (rs = –0.22, P <0.05). Q. rubra in the form of a mature tree had a negative influence on the cover of barochores, endozoochores and species of Grime’s CSR strategy. The cover of red oak’s seedlings was negatively correlated with the cover of annual species (rs = –0.21, P <0.05). Saplings of Q. rubra had a negative influence on the cover of dyszoochores (rs = –0.21, P <0.05) and was positively correlated with competitors (rs = 0.31, P <0.01), megaphanerophytes (rs = 0.27, P <0.05) and therophytes (rs = 0.25, P <0.05). The study showed that Q. rubra is a competitive species both as a mature tree and in the form of seedlings or small saplings and thus it can contribute to the reduction of biodiversity in forest communities.
Viola uliginosa (bog violet) is a declining species throughout its range due to – mostly anthropogenic – drying out of the wet habitats it occupies. Using AFLP markers, we aimed to estimate the genetic diversity in Polish populations, that may give an insight into the situation of plant populations facing rapid loss of natural habitats. Bog violet from several dispersed Polish populations is generally characterized by very low genetic diversity (HT = 0.048), even lower than several other endangered violets; therefore, we suggest that it should preserve at least EN rank in the red lists/red data books. The mean gene diversity within all populations (HS) was much lower than gene diversity (GST) between populations (0.020 versus 0.583, respectively) which supports the prevalence of clonal propagation of the species (mainly by stolons) but may also point to some significance of autogamy in cleisto- and chasmogamous flowers. A high FST value and the Mantel test for all populations revealed significant isolation by distance. Geographically neighboring pairs of populations formed genetic clusters supported by all (in the case of two closest populations) or most statistical analyses applied. Special attention should be paid to the locus classicus of the species in Rząska, consisting of a small number of individuals, forming a genetically distinct group, revealing very low gene diversity (Hj = 0.009) and the longest genetic distance to the remaining populations. Our results can contribute to planning future protection measures for the species at this and other locations. Genetic structure of the studied populations suggests local affinities of populations but does not generally support hypothesized recent continuity of V. uliginosa range along the river valleys of southern Poland; this view may, however, be altered with widening of the scope of studied populations and chosen molecular markers.
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