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Low-active high-density Noctiluca scintillans cells in surface seawater

100%

Tada K., Asahi T., Kitatsuji S., Nomura M., Yamaguchi H., Ichimi K.

Oceanologia

2020

tom 62

nr 3

Effects of Clostridium perfringens enterotoxin via claudin-4 on normal human pancreatic duct epithelial cells and cancer cells

84%

Yamaguchi H., Kojima T., Ito T., Kyuno D., Kimura Y., Imamura M., Hirata K., Sawada N.

Cellular and Molecular Biology Letters

2011

tom 16

nr 3

The tight junction protein claudin-4 is frequently overexpressed in pancreatic cancer, and is also a receptor for Clostridium perfringens enterotoxin (CPE). The cytotoxic effects of CPE are thought to be useful as a novel therapeutic tool for pancreatic cancer. However, the responses to CPE via claudin-4 remain unknown in normal human pancreatic duct epithelial (HPDE) cells. We introduced the human telomerase reverse transcriptase (hTERT) gene into HPDE cells in primary culture as a model of normal HPDE cells in vitro. hTERT-HPDE cells treated with or without 10% FBS and pancreatic cancer cell lines PANC-1, BXPC3, HPAF-II and HPAC were treated with CPE. In Western blotting, the expression of claudin-4 protein in hTERT-HPDE cells treated with 10% FBS was as high as it was in all of the pancreatic cancer cell lines. In hTERT-HPDE cells with or without 10% FBS, cytotoxicity was not observed at any concentration of CPE, whereas in all pancreatic cancer cell lines, CPE had a dose-dependent cytotoxic effect. In hTERT-HPDE cells with 10% FBS, claudin-4 was localized in the apical-most regions, where there are tight junction areas, in which in all pancreatic cancer cell lines claudin-4 was found not only in the apical-most regions but also at basolateral membranes. In hTERT-HPDE cells with 10% FBS after treatment with CPE, downregulation of barrier function and claudin-4 expression at the membranes was observed. In HPAC cells, the sensitivity to CPE was significantly decreased by knockdown of claudin-4 expression using siRNA compared to the control. These findings suggest that, in normal HPDE cells, the lack of toxicity of CPE was probably due to the localization of claudin-4, which is different from that of pancreatic cancer cells. hTERT-HPDE cells in this culture system may be a useful model of normal HPDE cells not only for physiological regulation of claudin-4 expression but also for developing safer and more effective therapeutic methods targeting claudin-4 in pancreatic cancer.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Oceanologia

2 Yamaguchi H.

1 Asahi T.

1 Hirata K.

1 Ichimi K.

1 Imamura M.

1 Ito T.

1 Kimura Y.

1 Kitatsuji S.

1 Kojima T.

1 Kyuno D.

1 Nomura M.

1 Sawada N.

1 Tada K.

1 2020

1 2011

Wyniki wyszukiwania

Low-active high-density Noctiluca scintillans cells in surface seawater

Effects of Clostridium perfringens enterotoxin via claudin-4 on normal human pancreatic duct epithelial cells and cancer cells