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Identification of genes encoding classical staphylococcal enterotoxins in Staphylococcus aureus isolated from raw milk

100%

Korpysa-Dzirba W., Osek J.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 1

This study describes a multiplex PCR assay developed for the detection of Staphylococcus aureus enterotoxin types: SEA, SEB, SEC, SED, and SEE presented in the literature as classical. The method was then used to analyse the presence of genes encoding these enterotoxins in .S aureus strains isolated from raw milk. A total of 237 raw milk samples were used in the study and 77 (32.5%) of them were found to be contaminated with S. aureus. Among them, five isolates were harbouring the genes encoding staphylococcal enterotoxins - type C (three strains) and type A (two strains). These results show that raw milk can potentially be a source of staphylococcal food poisoning.

2

Detection of classical genes and enterotoxins of Staphylococcus aureus isolated from raw milk in the south-east region of Poland

100%

Korpysa-Dzirba W., Osek J.

Bulletin of the Veterinary Institute in Puławy

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2014

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tom 58

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nr 4

The aim of the study was to investigate if the enterotoxigenic strains of S. aureus isolated from raw milk are able to produce staphylococcal enterotoxins (SEs) A - E. A total of 168 of S. aureus isolates from raw milk collected in the south - east region of Poland (Lubelskie Province) were tested for SE production by the ELFA, while multiplex PCR was applied for detection of enterotoxin genes (sea, seb, sec, sed, see). It was found that 20 (11.9%) out of 168 strains were positive for one or more classical SE markers and 19 of them produced a detectable level of enterotoxins. The results obtained by mPCR and ELFA were in agreement, when the presence of A, B, and D toxin types was tested; whereas SEC was not found by the ELFA although the S. aureus was positive for the respective gene. The results of the two methods showed that mPCR identified one more strain potentially producing enterotoxin than the ELFA, which may suggest that the enterotoxigenic S. aureus are not always able to express the toxin protein.

3

Homogeneity and stability of samples used for proficiency testing in enumeration of coagulase positive Staphylococci

81%

Korpysa-Dzirba W., Rola J. G., Osek J.

Bulletin of the Veterinary Institute in Puławy

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2012

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tom 56

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nr 4

This study describes preparation of test samples composed of freeze-dried strain of S. aureus and powdered milk as a matrix. In the first part of the study, the number of S. aureus cells freeze-dried in skim milk or horse serum were compared at two levels of contamination (10⁴ and 10⁵ cfu g⁻¹). The analysis of the samples was performed three times within a week. The preliminary results showed that the samples composed of S. aureus freeze-dried in horse serum were more stable and homogeneous than those prepared with skim milk. These results were further confirmed after analysing a higher number of such samples. Therefore, this procedure was then chosen for preparation of the samples for proficiency tests (PTs). Homogeneity and stability of these samples were checked according to ISO 13528. The results obtained showed that the samples met the criteria of stability and homogeneity required for PTs and were used in PT for enumeration of S. aureus in powdered milk.

4

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Enterotoksyny gronkowcowe. Część II. Europejska metoda skriningowa i jej modyfikacje - przyczyny i efekty

81%

Rola J. G., Korpysa-Dzirba W., Osek J.

Życie Weterynaryjne

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2012

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tom 87

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nr 09

5

Prevalence of Staphylococcus aureus and staphylococcal enterotoxins at different stages of production of raw milk cheeses - preliminary results

81%

Rola J. G., Korpysa-Dzirba W., Osek J.

Bulletin of the Veterinary Institute in Puławy

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2013

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tom 57

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nr 3

The aim of the study was to identify the potential sources of contamination of traditionally made cheeses during their production with Staphylococcus aureus. The samples were collected at nine dairy farms at different points of manufacturing the cheeses. Isolation and enumeration of coagulase positive staphylococci (CPS) on Baird- Parker RPF agar was conducted, and detection of staphylococcal enterotoxins (SEs) was performed using ELISA and ELFA. The genes encoding SEs were identified by PCR. CPS were isolated from 51 samples with the highest level of contamination in mature cheese up to 10⁷ CFU g⁻¹. No SEs were detected in tested samples; however, enterotoxic CPS strains were found.

6

Enterotoksyny gronkowcowe. Część I. Epidemiologia i znaczenie dla zdrowia publicznego

81%

Korpysa-Dzirba W., Rola J. G., Osek J.

Życie Weterynaryjne

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2012

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tom 87

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nr 08

7

Enterobacter sakazakii - zagrozenie mikrobiologiczne w zywnosci

81%

Korpysa-Dzirba W., Rola J. G., Osek J.

Medycyna Weterynaryjna

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2007

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tom 63

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nr 11

1277-1280

Enterobacter sakazakii - previously known s a yellow-pigmented E. cloacae - has, since 1980, been singled out as a unique species. This new classification is based on the differences from E. cloacae in DNA relatedness and biochemical reactions. Little is known about the presence of E. sakazakii in the environment. Most of the strains reported in literature have been isolated from clinical sources. Bacteria of Enterobacter species are considered to be opportunistic pathogens and rarely cause diseases in humans. In recent years there have been about 60 documented cases of E. sakazakii infections, but this number is probably underestimated because many clinical laboratories do not test for this microorganism and, in addition, an official reporting system has not been adopted in most countries. The populations at greatest risk of E. sakazakii infection are preterm, low-birth weight or immunocompromised infants, especially those hospitalized in neonatal intensive care units. The bacteria can cause life-threatening infections in neonates such as bacteraemia, enterocolitis or meningitis and survivors often suffer from neurological complications. Although the reservoir of E. sakazakii is unknown, the growing number of outbreaks of infection among this susceptible population has provided evidence that milk-based powdered infant formulas often serve as a source of infection. Unlike liquid formula products, dried milk powders are not sterile. In case of the presence of E. sakazakii, this microorganism can survive a long time on blenders, and, if not properly cleaned, can become a source of infection. The high mortality rate, the severity of the infection in infants and lack of information about the ecology and pathogenicity of this microorganism, has resulted in this bacteria becoming an object of interest to scientists worldwide.

8

Cronobacter sakazakii - charakterystyka i znaczenie w mikrobiologii żywności

61%

Sosnowski M., Czubkowska A., Korpysa-Dzirba W., Ostrowska M., Rola J. G., Osek J.

Medycyna Weterynaryjna

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2014

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tom 70

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nr 11

Cronobacter sakazakii has been separated from Enterobacter cloace. They are present in the environment and a wide variety of foods. Their presence in milk and infant formulas is a particular threat. Newborns, infants and immunocompromised adults are exposed to the infections caused by C. sakazakii. The bacteria are responsible for rare but life-threatening cases of sepsis, necrotizing enterocolitis, and purulent meningitis. They are usually sensitive to ampicillin, tetracycline, chloramphenicol, gentamicin, and third-generation cephalosporins; however, some strains are resistant to many antibiotics and a constant increase in antibiotic resistance is observed. C. sakazakii infections are more common in newborns and infants. The mortality caused by the presence of C. sakazakii is high and ranges from 40-80%. The mechanisms of virulence of this species are still in the research stage.

9

Sytuacja epidemiologiczna włośnicy w Polsce - I półrocze 2022 r.

51%

Belcik A., Korpysa-Dzirba W., Bilska-Zajac E., Rozycki M., Gontarczyk A., Kochanowski M., Samorek-Pierog M., Karamon J., Cencek T.

Życie Weterynaryjne

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2022

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tom 97

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nr 09

10

Gelatin medium for preserving of Trichinella spp. for quality control in laboratories - estimation of larvae viability

51%

Bilska-Zajac E., Rozycki M., Korpysa-Dzirba W., Belcik A., Karamon J., Sroka J., Samorek-Pierog M., Zdybel J., Wojcik-Fatla A., Cencek T.

Annals of Agricultural and Environmental Medicine

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2023

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tom 30

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nr 4

11

Molecular confirmation of the systemic toxoplasmosis in cat

51%

Sroka J., Wojcik-Fatla A., Bilska-Zajac E., Karamon J., Zdybel J. M., Piotrowska W., Dabrowska J., Samorek-Pierog M., Korpysa-Dzirba W., Cencek T.

Annals of Agricultural and Environmental Medicine

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2023

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tom 30

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nr 4

Ograniczanie wyników

Identification of genes encoding classical staphylococcal enterotoxins in Staphylococcus aureus isolated from raw milk

Detection of classical genes and enterotoxins of Staphylococcus aureus isolated from raw milk in the south-east region of Poland

Homogeneity and stability of samples used for proficiency testing in enumeration of coagulase positive Staphylococci

Enterotoksyny gronkowcowe. Część II. Europejska metoda skriningowa i jej modyfikacje - przyczyny i efekty

Prevalence of Staphylococcus aureus and staphylococcal enterotoxins at different stages of production of raw milk cheeses - preliminary results

Enterotoksyny gronkowcowe. Część I. Epidemiologia i znaczenie dla zdrowia publicznego

Enterobacter sakazakii - zagrozenie mikrobiologiczne w zywnosci

Cronobacter sakazakii - charakterystyka i znaczenie w mikrobiologii żywności

Sytuacja epidemiologiczna włośnicy w Polsce - I półrocze 2022 r.

Gelatin medium for preserving of Trichinella spp. for quality control in laboratories - estimation of larvae viability

Molecular confirmation of the systemic toxoplasmosis in cat