Wyniki wyszukiwania

1

Drozdze Saccharomyces cerevisiae w nauce i biotechnologii

100%

Ulaszewski S.

Biotechnologia

|

1994

|

nr 1

17-31

2

Interactions between the gene products of pma1 encoding plasma membrane H+-ATPase, and pdr1 controlling multiple drug resistance in Saccharomyces cerevisiae

100%

Ulaszewski S.

Acta Biochimica Polonica

|

1993

|

tom 40

|

nr 4

In Saccharomyces cerevisiae, the pmal mutations controlling the vanadate resistance of the H+-ATPase activity from the plasma membrane, map on chromosome VII in the vicinity of pdrl mutations controlling multiple drug resistance. However, the pmal-1 mutants exhibit a genotype and a multidrug resistant phenotype quite different from those obtained for pdrl mutants. Quantitative modifications of cycloheximide and N,N'-(p-xylylidene)-bis-aminoguanidine-2HCl resistance are observed in diploids containing the pmal and pdrl genes in trans configuration. Each of the pdrl mutations interacts with pmal as shown by a decrease in the ATPase activity in pdrl/pmal diploids. The in vitro resistance of ATPase activity to vanadate is totally or partially suppressed in pdrl mutants in haploid double mutants. These results suggest that the expression of PMA1 might be controlled by the PDR1 gene product.

3

Arsenical - induced transcriptional activation of the yeast Saccharomyces cerevisiae ACR2 and ACR3 genes requires the presence of the ACR1 gene product

63%

Bobrowicz P., Ulaszewski S.

Cellular and Molecular Biology Letters

|

1998

|

tom 03

|

nr 1

The yeast ACR1 gene encodes a putative transcriptional regulatory bZIP protein involved in the arsenical resistance. We demonstrate that the ACR2 and ACR3 genes are positively regulated by Acr1p. Changes in the ACR1 gene dosage influence the ACR2-lacZ and ACR3-lacZ fusion genes response to the arsenite induction. Arsenic and antimony compounds, but not bismuth and cadmium salts, strongly induced the expression of the ACR3 gene encoding the arsenite transporter related to the prokaryotic ArsB proteins.

4

Profesor Piotr Slonimski [9 XI 1922 - 25 IV 2009]

63%

Ulaszewski S.

Postępy Mikrobiologii

|

2009

|

tom 48

|

nr 2

89-91

5

N, N' (p-Xylylidene)-bis-aminoguanidine (2 HCl) resistant mutants in Saccharomyces cerevisiae and their relation to the amino acid transport system

51%

Witkowska R., Ulaszewski S., Lachowicz T. M.

Acta Microbiologica Polonica

|

1980

|

tom 29

|

nr 1

6

Rapid and easy detection of the five most common mutations in BRCA1 and BRCA2 genes in the Polish population using CAPS and ACRS-PCR methods

51%

Dabrowski A., Ulaszewski S., Niedzwiecka K.

Acta Biochimica Polonica

|

2019

|

tom 66

|

nr 1

7

Mutants of Saccharomyces cerevisiae resistant to a quaternary ammonium salt

51%

Oblak E., Ulaszewski S., Lachowicz T. M.

Acta Microbiologica Polonica

|

1988

|

tom 37

|

nr 3-4

8

The lethal effects of some olopnEhosphate insecticides on yeast Saccharomyces cerevisiae

51%

Ulaszewski S., Kolodynski J., Fekri A., Witek S.

Acta Microbiologica Polonica

|

1980

|

tom 29

|

nr 3

9

Mechanizm opornosci na zwiazki arsenu u prokariontow i ukariontow.

51%

Wysocki R., Bobrowicz P., Ulaszewski S.

Postępy Biochemii

|

1999

|

tom 45

|

nr 4

304-312

10

The physiological and morphological phenotype of a yeast mutant resistant to the quaternary ammonium salt N-[dodecyloxycarboxymethyl]-N,N,N-trimethyl ammonium chloride

51%

Oblak E., Gamian A., Adamski R., Ulaszewski S.

Cellular and Molecular Biology Letters

|

2010

|

tom 15

|

nr 2

We investigated the action of the quaternary ammonium salt (QAS) called IM (N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride) on Saccharomyces cerevisiae yeast cells. Changes in the yeast cell ultrastructure were confirmed by electron microscopy. We treated resistant mutant cells with QAS, and confirmed destruction of the mutant cytoplasm, an increase in the thickness of the cell wall, separation of the cell wall from the cytoplasm, and the accumulation of numerous lipid droplets. We also observed a relatively high production of lipids in the cells of the parental wild-type strain Σ1278b and in its IM-resistant (IMR) mutant in the presence of the QAS. The IMR mutant showed increased sensitivity to CaCl2 and SDS, and resistance to ethidium bromide, chloramphenicol, erythromycin and osmotic shock. It also tolerated growth at low pH. We suggest that the resistance to IM could be connected with the level of permeability of the cell membrane because the IMR mutant was sensitive to this compound in vivo in the presence of SDS and guanidine hydrochloride, which cause increased permeability of the cell plasma membrane.

11

Light effects in yeast: Relation between the respiratory deficiency and light sensitivity in yeast

51%

Ulaszewski S., Kolodynski J., Kotylak Z.

Acta Microbiologica Polonica

|

1982

|

tom 31

|

nr 3-4

12

Raport o stanie polskiej biotechnologii, 1995

51%

Zabza A., Ulaszewski S.

Biotechnologia

|

1995

|

nr 4

13-58

13

Transport of 3 - bromopyruvate across the human erythrocyte membrane

45%

Sadowska-Bartosz I., Soszynski M., Ulaszewski S., Ko Y., Bartosz G.

Cellular and Molecular Biology Letters

|

2014

|

tom 19

|

nr 2

3-Bromopyruvic acid (3-BP) is a promising anticancer compound because it is a strong inhibitor of glycolytic enzymes, especially glyceraldehyde 3-phosphate dehydrogenase. The Warburg effect means that malignant cells are much more dependent on glycolysis than normal cells. Potential complications of anticancer therapy with 3-BP are side effects due to its interaction with normal cells, especially erythrocytes. Transport into cells is critical for 3-BP to have intracellular effects. The aim of our study was the kinetic characterization of 3-BP transport into human erythrocytes. 3-BP uptake by erythrocytes was linear within the first 3 min and pH-dependent. The transport rate decreased with increasing pH in the range of 6.0–8.0. The Km and Vm values for 3-BP transport were 0.89 mM and 0.94 mmol/(l cells x min), respectively. The transport was inhibited competitively by pyruvate and significantly inhibited by DIDS, SITS, and 1-cyano-4-hydroxycinnamic acid. Flavonoids also inhibited 3-BP transport: the most potent inhibition was found for luteolin and quercetin.

14

Effect of some quaternary benzylammonium salts on physiology of yeast

39%

Kolodynski J., Ulaszewski S., Grobelny D., Witkowska R., Witek S., Lachowicz T.

Acta Microbiologica Polonica

|

1984

|

tom 33

|

nr 2

15

The YJL18C, YLR376C and YJR129C genes of Saccharomyces cerevisiae are probably involved in regulation of the glyoxylate cycle

39%

Boniewska-Bernacka E., Wysocki R., Grochowalska R., Machnicka B., Ulaszewski S., Lachowicz T.

Acta Biochimica Polonica

|

2006

|

tom 53

|

nr 4

Ograniczanie wyników

Drozdze Saccharomyces cerevisiae w nauce i biotechnologii

Interactions between the gene products of pma1 encoding plasma membrane H+-ATPase, and pdr1 controlling multiple drug resistance in Saccharomyces cerevisiae

Arsenical - induced transcriptional activation of the yeast Saccharomyces cerevisiae ACR2 and ACR3 genes requires the presence of the ACR1 gene product

Profesor Piotr Slonimski [9 XI 1922 - 25 IV 2009]

N, N' (p-Xylylidene)-bis-aminoguanidine (2 HCl) resistant mutants in Saccharomyces cerevisiae and their relation to the amino acid transport system

Rapid and easy detection of the five most common mutations in BRCA1 and BRCA2 genes in the Polish population using CAPS and ACRS-PCR methods

Mutants of Saccharomyces cerevisiae resistant to a quaternary ammonium salt

The lethal effects of some olopnEhosphate insecticides on yeast Saccharomyces cerevisiae

Mechanizm opornosci na zwiazki arsenu u prokariontow i ukariontow.

The physiological and morphological phenotype of a yeast mutant resistant to the quaternary ammonium salt N-[dodecyloxycarboxymethyl]-N,N,N-trimethyl ammonium chloride

Light effects in yeast: Relation between the respiratory deficiency and light sensitivity in yeast

Raport o stanie polskiej biotechnologii, 1995

Transport of 3 - bromopyruvate across the human erythrocyte membrane

Effect of some quaternary benzylammonium salts on physiology of yeast

The YJL18C, YLR376C and YJR129C genes of Saccharomyces cerevisiae are probably involved in regulation of the glyoxylate cycle