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The comparison of pollen abundance in air and honey samples

100%
Sauliene I., Sukiene L., Noreikaite-Merkeliene A., Pileckas V.
Acta Agrobotanica
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2015
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tom 68
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nr 4
Honey as a food has long been used in human nutrition and is still popular. Honey is important because of its therapeutic, prophylactic and strengthening value. Pollen is one of the most decisive components that ensure the quality and type of honey. Modern society becomes more and more sensitive to airborne pollen. Therefore, it is crucial to determine the composition of allergenic plant pollen in natural honey. For this purpose, we studied and compared pollen abundance in honey and air samples collected in Lithuania. Standard methods for pollen investigation in air and honey were used in this study. The botanical diversity of pollen identified in honey and air samples indicates 10 morphotypes: 8 of woody plants and 2 of herbaceous plants, in both the honey and air samples. Salix pollen counts in the honey were found to be highest among airborne pollen from May to September. The anemophilous allergenic pollen constituted 44 % of the total pollen detected in the honey.
2

Effect of different roe deer muscles on fatty acid composition in intramuscular fat

100%
Razmaite V., Siukscius A., Pileckas V., Svirmickas G. J.
Annals of Animal Science
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2015
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tom 15
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nr 3
3

Determination of optimum stallion semen freezing regimes

88%
Kutra J., Pileckas V., Urbsys A., Siukscius A., Nainiene R.
Medycyna Weterynaryjna
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2008
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tom 64
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nr 02
Semen cryopreservation opens new possibilities for the development of breeding work and preservation of the gene pool in horse breeding. Currently a relatively small number of mares are being inseminated with frozen semen. Therefore, the improvement of semen deep-freezing and thawing regimes is important for further studies of stallion semen cryopreservation processes. The methods of animal semen freezing on dry ice and in convectional liquid nitrogen vapor stream are carried out by simple technical means, and, what is more, large quantities of semen can be frozen at a time. However, programmed equipment involving a larger input allows the implementation and reproduction of desirable freezing regimes more accurately and to extend the range of their parameters. The semen was frozen in two ways: in convectional liquid nitrogen vapor stream on a metal perforated shield, fitted in biostorage KS-40 equipment and in Minicool AS-25. Three freezing regimes characterized by the super cooling temperature of the free water in the semen were realized using the Minicool AS-25 equipment. The efficiency of the freezing regimes was determined by post-thaw motility of spermatozoa. The results from the study indicated that the quality of frozen semen of most stallions was invariable regardless of freezing methods and regimes. Thus, semen can be frozen by convection flow of nitrogen vapor using common equipment and lower amounts of freezing agent. Optimum freezing regimes should be sought for cryopreservation of stallion semen known as “unstable freezing”. These regimes could be ensured by using the equipment suited to the reproduction of the identical freezing regime for every specific stallion’s semen. During the experiment there was only one stallion identified as having an optimum semen freezing regime, yet the experiment showed that in order to reach optimum conditions, a freezing parameter changing gradient could be determined in stallion semen cryopreservation. In the latter case the parameter characterizing freezing regimes could be the super freezing temperature of the free water in the semen and the optimum criterion could be post-thaw motility of spermatozoa.
4

Effects of zearalenone and product Toxy-Nil Plus Dry on physiological responses of boar semen

76%
Kutra J., Siukscius A., Urbsys A., Pileckas V., Nainiene R., Mankeviciene A.
Medycyna Weterynaryjna
|
2006
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tom 62
|
nr 01
The aim of the present study was to determine the effects of the mycotoxin zearalenone as well as the detoxicating product Toxy-Nil Plus Dry (TNPD) on the quality of boar semen. Thirteen Lithuanian White boars, 10 months of age and weighing 150 to 155 kg were used in the experiment and allotted to three groups. Every boar was offered 6 kg of compound feed containing 78% of barley meal and 22% of protein-vitamin-mineral premix (PVMP). Boars in the experimental groups were given feed containing zearalenone (0.57 mg/ /kg) for 32 days and detoxicated (1 kg TNPD per 1000 kg of feed) feeds. In a week following the administration of zearalenone-containing feeds to the boars, the volume of ejaculation had decreased by 40.8 % (P < 0.005) compared with the control group. The lowest initial spermatozoa motility in the semen collected during intoxication was determined in the group of boars receiving zearalenone-containing feed (3.9 ± 1.79 points, P = 0.01). On replacement of the contaminated feed, the motility of spermatozoa recovered within a week and amounted to 7.0 ± 0.55 points. The negative effect of TNPD on spermatozoa was noted during the recovery period. The total quantity of pathologic spermatozoa in a corresponding experimental group increased to 33.2 ± 8.75% compared with 21.7 ± 8.27% (P < 0.05) in the pre-experimental period. The study indicated that the investigated level of zaeralenone in feed negatively affects the reproductive performance of boars. The unhealthy effect of zearalenone may be significantly reduced by treating contaminated feeds with TNPD.
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