Wyniki wyszukiwania

1

Isolation of a WD40-repeat gene regulating anthocyanin biosynthesis in storage roots of purple-fleshed sweet potato

100%

Dong W., Niu L., Gu J., Gao F.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 05

Many transcriptional factors including the R2R3-MYB domain, basic helix-loop-helix (bHLH) domain and WD40 repeat proteins, which regulate flavonoid biosynthesis, have been identified in various plant species. However, there is little information on WD40 proteins in underground organs. In this study, a WD40-repeat protein gene was isolated from purple-fleshed sweet potato (Ipomoea batatas (L.) Lam. cv. Yamakawamurasaki) (IbWD40). The expression patterns of this gene were positively correlated with anthocyanin accumulation in different sweet potato cultivars. An IbWD40-GFP fusion protein was observed only in the nucleus of onion epidermal cells, which was consistent with its role as a transcriptional regulator. Stable transformation analysis revealed that IbWD40 was up-regulated in Arabidopsis thaliana seedlings, which accumulated anthocyanins, with possible additional effects on the formation of other flavonoid compounds in other tissues. These results suggest that in storage roots of purple-fleshed sweet potato the activity of IbWD40 plays a critical role in the regulation of anthocyanin biosynthesis.

2

Cadmium accumulation and its effects on growth and gas exchange in four populus cultivars

100%

Gu J., Qi L., Jiang W., Liu D.

Acta Biologica Cracoviensia. Series Botanica

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2007

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tom 49

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nr 2

7-14

3

GmSGR1, a stay-green gene in soybean (Glycine max L.), plays an important role in regulating early leaf-yellowing phenotype and plant productivity under nitrogen deprivation

88%

Shi S., Miao H., Du X., Gu J., Xiao K.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 04

4

Sequence analysis of the ORF7 region of chinese transmissible gastroenteritis virus isolate TGEs-1

76%

Lan D., Gu J., Xing R., Yuan C., Cui L., Hua X., Yang Z.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 2

Transmissible gastroenteritis virus (TGEV) was isolated from pigs suspected of having transmissible gastroenteritis (TGE) in China. The open reading frame 7 (ORF 7) of this isolate, designated as TGEs-1, which is located at the 3' end of the TGEV genome, was amplified by the RT-PCR. The amplified PCR product was cloned, sequenced, and compared with the sequences of 24 TGEV reference strains. TGEs-1 strain had 96.2%-100% nucleotide and 93.8%-100% amino acid sequence similarity with the other Chinese TGEV strains, and 92.4%-100% nucleotide and 92.5%-100% amino acid sequence similarity with non-Chinese TGEV strains. Interestingly, three virulent strains: TGEs-1, TS, and Miller showed a complete homology with H16 and H attenuated virulent strains in the nucleotide and amino acid sequences of ORF 7. In phylogenetic analysis of the ORF 7 gene, Chinese TGEV strains were clustered into three groups. TGEs-1 with three Chinese strains (TS, H16, and attenuated H) and one American strain (Miller) were clustered into single group. It should be noted that the Chinese and Asian TGEV strains were related to the TGEV strains isolated in the USA, and alien to the European strains.

5

Wheat miRNA member TaMIR2275 involves plant nitrogen starvation adaptation via enhancement of the N acquisition-associated process

76%

Qiao Q., Wang X., Yang M., Zhao Y., Gu J., Xiao K.

Acta Physiologiae Plantarum

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2018

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tom 40

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nr 10

6

The role of the GABA-A receptor of the adjacent intact dorsal root ganglion neurons in rats with neuropathic pain

64%

Ran R., Gu J., Fu J., Zhong H., Zhao Y., Gu Y. C., Zhang A., Li G., Wang L., Wang Q.

Acta Neurobiologiae Experimentalis

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2014

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tom 74

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nr 4

We aim to investigate the changes of The y-aminobutyric acid (GABA) signals in the adjacent intact dorsal root ganglion (DRG) and the contribution of these changes to the development and maintenance of neuropathic pain (NPP). After establishment of neuropathic pain model with the lumbar 5 spinal nerve ligation (L5 SNL), the GABA-evoked currents were recorded in the acutely dissociated L4 DRG neurons using whole-cell patch clamp. Moreover, Muscimol or Bicuculline were respectively topically injected to the L4 DRG at the time of nerve injury and post-operative 5 days (POD5). The pain thresholds of hind paw were recorded. We found that the incidence and amplitude of GABA currents significantly decreased in the large and medium L4 DRG neurons after SNL (P<0.05). Furthermore, in the L5 SNL rats, 20 ug Muscimol injected immediately after injury caused a long-lasting attenuation of mechanical allodynia (P<0.05), whereas the thermal hyperalgesia was not alleviated (P>0.05). 0.15 ug Bicuculline further decreased the pain thresholds (P<0.05) and reversed the pain-alleviating effects of Muscimol. However, at POD5, 20 ug Muscimol only exhibited transient alleviating effects (P<0.05). In conclusion, the decrease of inhibition signal mediated by GABA-A receptor in the adjacent intact DRG neurons may be crucial in the development of hyperalgesia in NPP.

7

TaPT2, a high-affinity phosphate transporter gene in wheat (Triticum aestivum L.), is crucial in Pi uptake under phosphorus deprivation

64%

Guo C., Guo L., Li X., Gu J., Zhao M., Duan W., Ma C., Lu W., Xiao K.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 06

Acquisition of inorganic phosphate (Pi) by plant roots is performed by phosphate transporters (PTs) located at the cytoplasmic membranes of epidermal cells and root hairs. A Triticum aestivum PT gene denoted as TaPT2 was functionally characterized in this study. TaPT2 is highly similar to TtPT2 and HvPT2, two PHT1 family genes in T. aestivum/Thinopyrum intermedium and barley, respectively. TaPT2 is 1,802 bp long at the cDNA level; it encodes a 525-amino acid polypeptide with a molecular weight of 57.5 kDa and an isoelectric point of 8.65. TaPT2 contains 12 conserved membrane-spanning domains and is transported to the cytosolic membrane after endoplasmic reticulum sorting. Functional complement analysis revealed that TaPT2 endowed Pi transporter activities in a yeast mutant that is defective in Pi uptake, with highaffinity Pi acquisition. TaPT2 transcripts were specifically detected in the roots. The transcripts were upregulated under Pi deprivation and downregulated under Pi sufficiency. These results suggest that TaPT2 expression is associated with external Pi concentration. Transgene analysis revealed that TaPT2 overexpression or knockdown did not regulate plant dry mass production, Pi acquisition, and photosynthetic capacity under Pi sufficiency. Under Pi deprivation, TaPT2 overexpression increased plant dry mass accumulation, total P content per plant, and photosynthetic efficiencies, whereas TaPT2 downregulation reduced plant dry mass, accumulative P amount, and photosynthetic parameters. These results collectively suggest that TaPT2 is a high-affinity PHT1 member that has important functions in mediating plant Pi uptake under Pi deprivation. TaPT2 can serve as a useful gene resource for the improvement of phosphorus use efficiency in cereals under Pi deprivation.

8

Nitrate reutilization mechanisms in the tonoplast of two Brassica napus genotypes with different nitrogen use efficiency

64%

Han Y., Liao Q., Yu Y., Song H., Liu Q., Rong X., Gu J., Lepo J. E., Guan C., Zhang Z.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 02

Nitrate (NO3¯) can accumulate in high concentrations in plant cell vacuoles if it is not reduced, reutilized or transported into the cytoplasm. Such accumulation of NO3¯ in the vacuole occurs when mechanisms for NO3¯ assimilation in the cytoplasm are saturated. Moreover, other processes such as efflux across the plasma membrane might affect NO3¯ accumulation in the vacuole. These are the main reasons limiting nitrogen use efficiency (NUE) in plants. This study elucidates mechanisms for NO3¯ transport from the cytoplasm to vacuoles by the V-proton pump (V-ATPase and V-PPase) and their relationship with different NUE in four Brassica napus genotypes. Pot experiments were conducted in a greenhouse under normal (15.0 mmol L-1) and limited N (7.5 mmol L-1) concentrations of nitrate using B. napus genotypes that demonstrated either high (742 and Xiangyou 15) or low (814 and H8) NUE (g g-1). Specific inhibitors of V-ATPase and V-PPase increased nitrate reductase (NR) activity, resulting in greatly decreased NO3¯ in plant tissues. Nitrate reductase activity and NO3¯ content correlated more highly to V-PPase activity than they did to V-ATPase activity, and correlation between V-PPase activity and NO3¯ content was significantly higher than it was to V-ATPase. Genotypes with high NUE had significantly lower activities of V-ATPase and V-PPase than those with low NUE. In the high-NUE plants, lower activities of V-proton pump underlie mechanisms that result in significantly lower NO3¯ content in plant tissues of the high-NUE genotypes than those found in plant tissues of the low-NUE genotypes. Our results show that the tonoplast proton pumps V-PPase and V-ATPase strongly negatively affect NR activity and positively affect NO3¯ content. V-PPase contributed more to this regulatory mechanism than did V-ATPase.

9

Isolation and expression pattern of RGS21 gene, a novel RGS member

64%

Li X., Chen L., Ji C., Liu B., Gu J., Xu J., Zou X., Gu S., Mao Y.

Acta Biochimica Polonica

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2005

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tom 52

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nr 4

Regulators of G-protein signaling (RGS) proteins are known for the RGS domain that is composed of a conserved stretch of 120 amino acids, which binds directly to activated G-protein α subunits and acts as a GTPase-activating protein (GAP), leading to their deactivation and termination of downstream signals. In this study, a novel human RGS cDNA (RGS21), 1795 bp long and encoding a 152-amino acid polypeptide, was isolated by large-scale sequencing analysis of a human fetal brain cDNA library. Unlike other RGS family members, RGS21 gene has no additional domain/motif and may represent the smallest known member of RGS family. It may belong to the B/R4 subfamily, which suggests that it may serve exclusively as a negative regulator of αi/o family members and/or αq/11. PCR analysis showed that RGS21 mRNA was expressed ubiquitously in the 16 tissues examined, implying general physiological roles.

Ograniczanie wyników

Isolation of a WD40-repeat gene regulating anthocyanin biosynthesis in storage roots of purple-fleshed sweet potato

Cadmium accumulation and its effects on growth and gas exchange in four populus cultivars

GmSGR1, a stay-green gene in soybean (Glycine max L.), plays an important role in regulating early leaf-yellowing phenotype and plant productivity under nitrogen deprivation

Sequence analysis of the ORF7 region of chinese transmissible gastroenteritis virus isolate TGEs-1

Wheat miRNA member TaMIR2275 involves plant nitrogen starvation adaptation via enhancement of the N acquisition-associated process

The role of the GABA-A receptor of the adjacent intact dorsal root ganglion neurons in rats with neuropathic pain

TaPT2, a high-affinity phosphate transporter gene in wheat (Triticum aestivum L.), is crucial in Pi uptake under phosphorus deprivation

Nitrate reutilization mechanisms in the tonoplast of two Brassica napus genotypes with different nitrogen use efficiency

Isolation and expression pattern of RGS21 gene, a novel RGS member