Wyniki wyszukiwania

1

Non-gel based techniques for plant pathogen genotyping

100%

Abd-Elsalam K. A.

Acta Microbiologica Polonica

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2003

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tom 52

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nr 4

2

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Bacillus-based biological control of cotton seedling disease complex

51%

Khiyami M. A., Omar M. R., Abd-Elsalam K. A., Abd El-Hady Aly A.

Journal of Plant Protection Research

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2014

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tom 54

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nr 4

To formulate an efficient and eco-friendly strategy for the management of cotton seedling disease complex, pot experiments were conducted and the efficiency of eight Bacillus strains against seven fungi involved in the disease were determined. A greenhouse evaluation of the interaction between fungal isolates and Bacillus strains was carried out. The evaluation revealed a very highly significant Bacillus strains x fungal isolates interaction for all the following parameters: preemergence damping-off, postemergence damping-off, survival, plant height, and dry weight. This interaction implies that a single strain of the Bacillus sp. can be highly effective against a fungal isolate, but may have only minimal effects on other fungal isolates. The results of the present study demonstrated that Bacillus circulans and B. coagulans were the most effective strains in controlling cotton seedling disease. Therefore, strains of Bacillus spp. should be tested against as many fungal isolates as possible. The testing will improve the chance of identifying Bacillus strains effective against several fungal isolates.

3

Differential antagonism of Trichoderma sp. against Macrophomina phaseolina

51%

Aly A. A., Abdel-Sattar M. A., Omar M. R., Abd-Elsalam K. A.

Journal of Plant Protection Research

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2007

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tom 47

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nr 2

In view of the ecological hazards of chemicals, pot experiments were conducted to determine the efficacy of Trichoderma sp. against Macrophomina phaseolina. Greenhouse evolution of the interaction between M. phaseolina isolates and Trichoderma sp. (isolated revealed a very highly significant (p=0.0000). M. phaseolina isolate x antagonist isolate interaction for all the following parameters: preemergence damping-off, postemergence damping-off, survival, plant height, and dry weight. This interaction implies that a single isolate of antagonist can be highly effective against an isolate of M. phaseolina, but may have only minimal effects on other isolates of M. phaseolina. Therefore, isolates of antagonist should be tested against as many isolates of M. phaseolina as possible, as this will improve the chance of identifying antagonist isolates effective against several isolates of M. phaseolina.

4

Comparative assessment of genotyping methods for study genetic diversity of Fusarium oxysporum isolates

45%

Abd-Elsalam K. A., Guo J. R., Schnieder F., Asran-Amal A. M., Verreet J. A.

Polish Journal of Microbiology

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2004

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tom 53

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nr 3

In this study, we evaluated three PCR-based methods for the molecular typing of nonpathogenic Fusarium oxysporum isolates: random amplified polymorphic DNA (RAPD), polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and amplified fragment length polymorphism (AFLP). The analyses were performed using 64 isolates of F. oxysporum collected from cotton-producing areas in Egypt. A number of polymorphic RAPD, PCR-RFLP and AFLP bands were scored in all isolates and the genetic similarity among them was assessed. Clustering analysis separated the isolates into two main groups, with similarities ranging from 87 to 100% for RAPD, 80 to 100% for PCR-RFLP and 88 to 97% for AFLP, respectively. The obtained data suggested that all three types of markers are equally informative, but the three assays differed in the amount of detected polymorphic bands. AFLP fingerprinting was also found to be more differentiating than other techniques for the typing of F. oxysporum populations.

Ograniczanie wyników

2 Journal of Plant Protection Research

1 Acta Microbiologica Polonica

1 Polish Journal of Microbiology

4 Abd-Elsalam K. A.

2 Omar M. R.

1 Abd El-Hady Aly A.

1 Abdel-Sattar M. A.

1 Aly A. A.

1 Asran-Amal A. M.

1 Guo J. R.

1 Khiyami M. A.

1 Schnieder F.

1 Verreet J. A.

1 2014

1 2007

1 2004

1 2003

Non-gel based techniques for plant pathogen genotyping

Bacillus-based biological control of cotton seedling disease complex

Differential antagonism of Trichoderma sp. against Macrophomina phaseolina

Comparative assessment of genotyping methods for study genetic diversity of Fusarium oxysporum isolates