Photosynthetic rate, chlorophyll fluorescence, leaf nitrogen and chlorophyll content of Cypripedium flavum were studied at different leaf ages. The photosynthetic capacity changed significantly with leaf age. Net photosynthesis and chlorophyll content peaked when leaf age was 60 days, decreasing at 30, 90 and 120 days. Stomatal conductance showed the highest value at 60 days, while mesophyll conductance decreased with increasing leaf age.Both leaf nitrogen content per unit area and leaf nitrogen content per unit mass decreased with increasing leaf age. The age-dependent variation in photosynthetic capacity could be linked to the changes in biochemical efficiency, leaf nitrogen content and CO2 diffusion limitation.
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