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Response to oxidative stress induced by cadmium and copper in tobacco plants (Nicotiana tabacum) engineered with the trehalose-6-phosphate synthase gene (AtTPS1)

100%

Martins L. L., Mourato M. P., Baptista S., Reis R., Carvalheiro F., Almeida A. M., Fevereiro P., Cuypers A.

Acta Physiologiae Plantarum

2014

tom 36

nr 03

The response of tobacco plants genetically engineered with the AtTPS1 gene to stress induced by excess Cu and Cd was evaluated in hydroponic solution (100 and 400 μM Cu and 50 and 200 μM Cd) after a 48 h exposure. Two transgenic lines, transformed with the AtTPS1 (trehalose-6-phosphate synthase) gene from Arabidopsis, with different levels of trehalose-6-phosphate synthase expression (B5H, higher and B1F, lower), and a wild type (WT) were investigated. Protein content, antioxidative enzymes (CAT, POD, SOD, and APX), glucose, fructose, lipid peroxidation, hydrogen peroxide and Cd and Cu contents were determined in leaves. The two transgenic lines were differently influenced by Cd and Cu exposure as they induced a different antioxidant enzymatic defense response. B1F and B5H plants showed a better acclimation to Cd and excess Cu compared to WT. Furthermore B1F was more tolerant than B5H to Cd and excess Cu. B1F accumulated less Cd and Cu in leaves, probably due to a more efficient exclusion mechanism. Catalase was shown to be the most important enzyme in the antioxidative system of these plants.

Electroporation of maize embryogenic calli with the trehalose-6-phosphate synthase gene from Arabidopsis thaliana

100%

Almeida A. M., Villalobos E., Araujo S. S., Cardoso L. A., Santos D. M., Fevereiro P. S., Torne J. M., Santos M. A.

Acta Physiologiae Plantarum

2007

tom 29

nr 3

Trehalose is a non-reducing disaccharide of glucose that occurs in a large number of organisms, playing an important role in desiccation and heat stress protection. Trehalose accumulation has proven to be an effective way of increasing drought tolerance in both model plants such as tobacco and important crops such as potato or rice. In this work we aim to genetically engineer maize with the Arabidopsis thaliana trehalose phosphate synthase gene (AtTPS1), involved in trehalose biosynthesis via electroporation. A cassette harboring the AtTPS1 gene under the control of the CaMV35S promoter and the Bialaphos resistance gene Bar as a selective agent was inserted in the plasmid vector pGreen0229 and used to transform maize inbred line Pa91 via electroporation. Fifteen putative transgenic plants (T0 generation) were obtained. Transgene integration in T0 plants was analyzed by Southern-blot analysis. T0 plants had normal phenotypes, although smaller than wild type plants. Contrary to wild type plants, when sexual organs emerged, tassels appeared at least 15 days earlier than ears in the same plant, rendering impossible the self-pollination of the T0 plant. These plants were then crossed with wild type plants and in some cases T1 seeds were obtained. T1 seeds presented deformities, especially the lack of endosperm, but it was still possible to germinate some of these seeds. The so obtained plants were tested by Northern blot but no AtTPS1 gene expression was detected, a fact possibly due to the incomplete insertion of the AtTPS1 gene or an extremely low gene expression level.

Ograniczanie wyników

2 Acta Physiologiae Plantarum

2 Almeida A. M.

1 Araujo S. S.

1 Baptista S.

1 Cardoso L. A.

1 Carvalheiro F.

1 Cuypers A.

1 Fevereiro P.

1 Fevereiro P. S.

1 Martins L. L.

1 Mourato M. P.

1 Reis R.

1 Santos D. M.

1 Santos M. A.

1 Torne J. M.

1 Villalobos E.

1 2014

1 2007

Wyniki wyszukiwania

Response to oxidative stress induced by cadmium and copper in tobacco plants (Nicotiana tabacum) engineered with the trehalose-6-phosphate synthase gene (AtTPS1)

Electroporation of maize embryogenic calli with the trehalose-6-phosphate synthase gene from Arabidopsis thaliana