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The developed eastern coastal cities of China are leaders of the national economy, which is growing rapidly. However, with this growth has come substantial misuse of resources causing significant environmental damage, including some 85% of global greenhouse gases resulting from the impact of economic development. To explore a reasonable approach to coordinated development of economic growth and the environment, this paper assesses the city of Qingdao, employing regression analysis and other methods to establish an economic-energy-carbon emissions-social system dynamics model with a new industrial structure based on the degree of pollution. On the basis of new industrial structures, three separate schemes are formed by different combinations of sensitive variables. The research shows that over-emphasis on the environment or production of control measures are not appropriate under the current economic modality. Overemphasis on the environment will lead to rapid decline in economic growth and even the loss of economic growth momentum. Over-emphasis on production will result in a sharp increase in carbon emissions, accelerating the destruction of the environment and causing irreparable losses. Only by taking moderate measures on the basis of the current economic conditions can we achieve the desired economic and ecological goals.
Adjuvant chemotherapy has become the frequently adopted standard therapeutic approach for non-small cell lung cancer (NSCLC). However, the development of multidrug resistance (MDR) is a major obstacle contributing to the failure of chemotherapy. This study aimed to identify genes associated with MDR development that predict tumor response to chemotherapy in NSCLC. In the present study, a multidrug-resistant NSCLC cell sub-line, A549/MDR, was established from the A549/DDP cell line and characterized. The resistance index (RI) of this subline was calculated according to the IC50 of A549/MDR relative to the parental A549/DDP cells. The gene expression profiles of A549/DDP and A549/MDR were obtained using an oligonucleotide microarray (Agilent SureHyb microarray chip). The microarray results were validated by qRT-PCR and selected genes were analyzed by in vitro loss-of-function experiments. Gene expression profiling identified 921 differentially expressed genes (DEGs) according to the selection criteria, in which 541 genes were upregulated and 380 genes were downregulated in A549/MDR compared with A549/DDP cells. We found that these DEGs are involved in diverse biological processes, including ribonucleoprotein complex, drug metabolism, the Hippo signaling pathway and transcriptional misregulation. NOLC1, as one of the identified DEGs, was confirmed to be overexpressed in A549/MDR cells and its knockdown significantly enhanced the drug sensitivity of A549/MDR cells in response to multidrug treatment. Furthermore, knockdown of NOLC1 downregulated the expression levels of drug resistance-associated molecules (LRP and MDR1) in A549/MDR cells. These findings provide a new and comprehensive expression profile of MDR in NSCLC cells. Identification and validation of NOLC1 might be a promising therapeutic strategy for the management of MDR of NSCLC patients.
The objective of this study was to investigate the effect of Selenium (Se) supply (0, 3, 6, 12, 24 mg kg−1) on the growth, photosynthetic characteristics, Se accumulation and distribution of flue-cured tobacco (Nicotiana tabacum L.). Results showed that low-dose Se treatments (≤6 mg kg−1) stimulated plant growth but high-dose Se treatments (≥12 mg kg−1) hindered plant growth. Optimal Se dose (6 mg kg−1) stimulated plant growth by reducing MDA content and improving photosynthetic capability. However, excess Se (24 mg kg−1) increased MDA content by 28%, decreased net photosynthetic rate and carboxylation efficiency by 34% and 39%, respectively. The Se concentration in the roots, stems, and leaves of the tobacco plants significantly increased with increasing Se application. A linear correlation (R = 0.95, P < 0.01) was observed between Se level and tobacco plant tissue Se concentration. This correlation indicated that the tobacco plant tissues were not saturated within the concentration range tested. The pattern of total Se concentration in the tobacco plant tissues followed the order root > leaf > stem. The Se concentration in the roots was 3.17 and 7.57 times higher than that in the leaves and stems, respectively, after treatment with 24 mg kg−1 Se. In conclusion, the present study suggested that optimal Se dose (6 mg kg−1) improved the plant growth mainly by enhancing photosynthesis, stomatal conductance, carboxylation efficiency and Rubisco content in the flue-cured tobacco leaves. However, the inhibition of excess Se on tobacco growth might be due to high accumulation of Se in roots and the damage of photosynthesis in leaves.
Biomass allocation pattern is an important plant characteristic which influences how plants respond to abiotic and biotic heterogeneity. Prior studies indicate that above-ground biomass scales nearly isometrically with respect to below-ground biomass regardless of environment or phyletic affinity. However, such rule has been mostly tested with data on trees and usually without drought stress. Given the importance of this predicted relationship, it should be evaluated for a wider range of species and environmental conditions. Variations of the above- and belowground biomass (MA and MR, respectively) were determined from five sites in north-west China, which compose a natural moisture gradient (aridity index ranging from 0.95 to 1.98). Model Type II regression protocols were used to compare the numerical values of MA vs MR scaling exponents (i.e. slopes of log-log linear relationships). The resulting five scaling exponents were indistinguishable and had a similar, nearly isometric slope (i.e. MA ∞ MR ͌ ¹‧⁰). Significant variation was observed in the Y-intercepts of the five regression curves, because of the absolute differences in MA or MR. These results support prior allometric theory, which reveals an isometric relationship between above- and below-ground biomass, and may provide a suitable method to estimate the regional below-ground biomass based on the direct aboveground measurements.
The scaling relationship between the number and size of plant’s components has been observed traditionally as reflective of a trade-off in resource allocation over ontogeny. The recent finding of a negative isometric leaf size/number trade-off across 24 deciduous woody species extends knowledge of such trade-offs to current-year shoots. Before generally accepted, this isometry has to be consistent across more datasets that represent diverse habitats. We tested this scaling relationship using 12 deciduous shrub species from the western Gobi Desert and 56 woody species from the northeastern temperate zone of China. Our results showed that leaf number per stem mass of current-year shoots scaled approximately isometrically with individual leaf mass within and across habitats, which, combined with the independence of total leaf mass and individual leaf mass, supports isometric scaling for shoot-scale leaf deployment. However, the intercepts of these relationships decreased significantly along the environmental gradients, suggesting that habitats could place a constraint on the total leaf number that can be supported by a given size shoot. Convergence towards higher leaf number and smaller leaf size for some desert species suggests important adaptive implications for photosynthetic carbon gain and reproductive growth.
Eucommia ulmoides Oliver is rich in trans-polyisoprene rubber (Eu-Rubber), a high-molecular mass polymer of isoprene units with a trans-configuration. Farnesyl diphosphate (FPP) synthase (FPS) is a key enzyme, which involved in the production of important precursors of different terpenoids. In this study, we cloned and characterized five novel FPS genes from E. ulmoides. The full-length synthases were named EuFPS1-5 and their deduced amino acid sequences exhibited high homology to those from other plant isoforms. EuFPS1 and EuFPS4 were observed to be highly expressed in leaves, EuFPS2 and EuFPS3 were present at low levels in leaves and fruit throughout the plant development, and EuFPS5 was highly expressed exclusively in young fruit. Expression of EuFPS5 correlated with the accumulation rate of Eu-Rubber and might be responsible for it. This study is expected to enhance our understanding of the role of EuFPSs in biosynthesis and regulation of useful secondary metabolites in E. ulmoides.
Powdery mildew disease caused by Blumeria graminis f. sp. tritici (Bgt) is an economically important disease in wheat worldwide. The identification of germplasms resistant to the disease can not only facilitate the breeding of resistant cultivars, but can also broaden the diversity of resistance genes. The Mexican M53 is a synthetic hexaploid wheat line developed at the International Maize and Wheat Improvement Center (CIMMYT) from the cross between Triticum durum and Aegilops tauschii249. Infection of M53 with 15 different pathogen races revealed that the resistance in M53 was race-dependent and effective against the majority of the tested Bgt races, including the race 15 predominant in the Beijing wheat growing area. Inoculation of the parents of M53 with the race 15 demonstrated that M53 and Ae. tauschii249 were resistant, whereas T. durum was susceptible. The inoculation of three segregating F2 populations developed from the crosses between M53 and three susceptible Chinese wheat cultivars with the race 15 showed that the resistant gene in M53 segregated in a single dominant manner. Amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers were used to map the gene in a segregating F2 population consisting of 213 lines developed from the cross Wan7107 × M53. Two closely linked AFLP markers, Apm109 and Apm161, were identified to flank the gene with genetic distances of 1.0 cM and 3.0 cM, respectively. The recognized gene was assigned to the long arm of chromosome 5D as determined by three linked SSR markers, Xwmc289b, Xgwm583, and Xgwm292, and by the physical mapping of Apm109 using Chinese Spring nullisomic–tetrasomic and ditelosomic stocks. The resistance gene identified in M53, temporarily designated as Pm-M53, could be used in local wheat-breeding programs to improve powdery mildew resistance.
The Changjiang Delta Region is a rapidly urbanizing area in China. But this area still reveals different anthropogenic activities and urbanization levels. River sediments from urban, suburban, and rural areas were studied to characterize potentially harmful elements (PHEs) and their ecological risks. Chemical compositions of sediments were analyzed, which revealed pronounced differences in three areas. Al₂O₃, Fe₂O₃, CaO, and MgO were elevated in the urban sediments. Sediments from the rural area showed high organic matter. CaO and TOC of sediments fluctuated significantly in the suburban area. They are associated with the local geological provenance and sediment circumstance. The enrichments of PHEs in sediments of urban rivers were prevalent, among which Cd and As were prominent. Concentrations of PHEs in the suburban area fluctuated significantly. Lead and Cu were obviously enriched in this area. Rural sediments had relatively low concentrations of PHEs, which were also stable in the regional distribution, although As showed a slight enrichment. Based on RI values from Hakanson, the urban sediments showed moderate to considerable ecological risk. And the suburban sediments were in moderate ecological risk, except some high-risk samples. The majority of rural sediments revealed low ecological risk. Of these hazardous elements, Cd, Hg, and Pb contribute the largest proportion of the total ecological risk. It is evident that the urbanization level influences the distributions and contamination grades of PHEs for river sediments of the Changjiang Delta Region.
Selenium deficiency in crops has become a subject of growing concern where soil Se concentration is low. The mechanisms of Se translocation in the soil-rice system is very complex and the influence of heavy metal elements and nutrient concentrations on Se translocation in the soil-rice system is unknown. Our study investigated concentrations of Se, heavy metals like Hg, Cd, and Pb, and nutrient elements like Ca, K, P, and S in soils and rice tissues (roots, stems, and grains) in different industrial regions in Jiangsu Province, China. The transfer of Se in the soil-rice system was calculated by transfer coefficients (TC Se) in this study. The results showed that Se transfer from soil to roots and from stems to grain were key steps for controlled Se concentration in rice grains. The multiple linear regression analysis makes an implication that some elements influence the Se transfer significantly: soil K and Hg may suppress Se entering rice roots; B, Cu, and Mo in rice root may restrain Se transfer from root to stem; and S, Cr, P, and Mg in rice stem had negative effects on Se accumulation in rice grain. Therefore, reducing heavy metal pollution and managing fertilizer amounts may elevate Se concentration in rice grain, especially when Se concentration in soils is low.
Background: Laryngeal cancer tends to have a very poor prognosis due to the unsatisfactory efficacy of chemotherapy for this cancer. Multidrug resistance (MDR) is the main cause of chemotherapy failure. The proto-oncogene c-fos has been shown to be involved in the development of MDR in several tumor types, but few studies have evaluated the relationship between c-fos and MDR in laryngeal cancer. We investigated the role of c-fos in MDR development in laryngeal cancer cells (cell line: human epithelial type 2, HEp-2) using the chemotherapeutic vincristine (VCR). Methods: HEp-2/VCR drug resistance was established by selection against an increasing drug concentration gradient. The expressions of c-fos and multidrug resistance 1 (mdr1) were measured using qPCR and western blot. C-fos overexpression or knockdown was performed in various cells. The intracellular rhodamine-123 (Rh-123) accumulation assay was used to detect the transport capacity of P-glycoprotein (P-gp, which is encoded by the mdr1 gene). Results: HEp-2 cells with VCR-induced resistance (HEp-2/VCR cells) were not only resistant to VCR but also evolved cross-resistance to other chemotherapeutic drugs. The expressions of the c-fos and mdr1genes were significantly higher in the HEp-2/ VCR cells than in control cells. C-fos overexpression in HEp-2 cells (c-fos WT) resulted in increased P-gp expression and increased the IC50 for 5-FU. C-fos knockdown in the HEp-2/VCR cells (c-fos shRNA) resulted in decreased P-gp expression and decreased IC50 for 5-FU. An intracellular Rh-123 accumulation assay showed that the mean intracellular fluorescence intensity (MFI) was lower in the HEp-2/VCR cells than in HEp-2 cells. C-fos WT cells also showed lower MFI. By contrast, c-fos shRNA cells exhibited a higher MFI than the control group. Conclusion: C-fos increased the expression of P-gp and mdr1 in the HEp-2/VCR cells, and enhanced the efflux function of the cells, thereby contributing to the development of MDR.
Suitable reference gene (RGs) is the prerequisite for accurate normalization of real-time quantitative PCR (RT-qPCR) data. However, previous results are diverse in various researches that focused on selecting stable RGs. This study aims at systematically assessing various RGs in plants under salt stress or drought stress by collection of geNorm rankings of genes, data transformation and statistic analysis. Although none of the analyzed genes can guarantee universally stable expressions in plant species under salt stress or drought stress, we found that 18S (18S ribosomal RNA) was generally the least stable gene under salt and drought stress. This gene should not be used as the RG in RT-qPCR. On the contrary, it is least risk to use EF1 for salt stress and TIP41 for drought treatment experiments. We compared the effects of salt and drought stresses on 7 frequently used RGs through paired-samples T test. The expression of Ubiquitin gene under drought stress is much more unstable than that under salt stress. The tested genes belonging to multigene family and having different stability could be one reason of variations in the published studies, which was supported by the analysis of expression profile of Salicornia europaea transcriptome. This is the first systematic assessment quantifying global stability of Rgs across plant species under salt stress and drought stress, which will improve our understanding of RGs and facilitate the future work on RGs selection.
The hydrogen sulfide (H₂S) in biogas is poisonous and corrosive, so it is usually removed in the early stage of biogas upgrading. Dosing iron compounds directly into the anaerobic fermenter is an in-situ method for rough desulphurization. But it is difficult to estimate the appropriate amount of iron compound to add and overdosing is usually inevitable. Five kinds of iron compounds (FeCl₂, FeCl₃, Fe(OH)₃, Fe₂O₃, and FeSO₄) were applied as in-situ desulfurizers in chicken manure fermentation to reduce H₂S emissions. Biogas yield, CH₄ concentration, and H₂S concentration were examined to evaluate the performance of these desulfurizers. Among these five desulfurizers, FeCl₂, FeCl₃, and Fe(OH)₃ showed better performance; the desulfurization rates were all above 98.5% when the addition was 16 mmol L⁻¹. In order to establish the prediction model of the required amount for in-situ desulfurizer, it is assumed that the dosage of desulfurizer could be simply divided into two parts: one part for consumption of released H₂S, and the other part for guaranteeing a certain desulfurizing level. Under this assumption, the prediction formulas were fitted based on the bottle experiments and applied in a 5 L fermentation system. The required desulfurization levels (H₂S concentration) when adding FeCl₂, FeCl₃, and Fe(OH)₃ were set to 120, 200, and 100 ppmv, respectively. After adding the calculated dosage of the three in-situ desulfurizers, the actual H₂S concentrations were 163.0, 180.3, and 89.4 ppmv, respectively, which were relatively closed to the required desulfurization levels.
Brown cotton is a kind of naturally colored cotton. Because of less processing and little dying, it is more friendlier to environment than white cotton. For brown cotton, pigment accumulation in fiber is one of the most important characteristics. In this study, we selected a brown fiber line and a white fiber cultivar to determine the factor that affects the pigmentation in brown fiber. Accordingly, fibers were collected to verify the presence of PAs by p-dimethylaminocinnamaldehyde (DMACA) and toluidine blue O (TBO) staining. The PAs content and related genes expressions were determined. As a result, there were obvious differences on the aspect of PAs synthesis in fiber between white cotton and brown cotton. For white fiber, the PAs content reached maximum at 5 DPA, and then gradually decreased to zero. But for brown fiber, the PAs content was increased from 5 to 15 DPA stage, and reached the maximum at the 15 DPA stage, then gradually decreased from 15 to 40 DPA stage. On the contrary, in white cotton, PAs were synthesized in the whole developmental stage from 5 to 40 DPA. And PAs content in brown fiber were far more than that in white fiber, which may be the reason why the brown pigment accumulated in brown fiber.
Pattern of plant biomass and net primary production was investigated in two localities (Minqin and Linze) of oasis-desert ecotone (ODE) in Northwest China, in order to recognize the spatial and temporal variability of vegetation under same regional climate with different groundwater depth. The average depth to groundwater was over 14.02 m at Minqin -- marked further as DG (deep groundwater) and about 4.96 m at Linze -- marked further as SG (shallow groundwater). We have measured plant biomass and Netprimary productivity (NPP) across species, threetimes per year for three consecutive years, in sixplots along Minqin and Linze oasis-desert ecotone(further marked as DG and SG ODE), respectively.Our results showed that DG and SGODEs had different growth responses to differentgroundwater depths. DG ODE exhibited higherinter-annual variation in annual NPP (rangedfrom 0.18 to 9.30 g m⁻²) than did SG ODE (rangedfrom 0.42 to 17.99 g m⁻²). Decrease of groundwaterdepth had apparently altered the seasonalityof productivity in DG ODE systems, where precipitationin summer maintained plant growth,while ODE with high groundwater depth tendedto have higher spring NPP in SG ODE. Spatialand temporal heterogeneity of NPP at the scaleof our measurements was significantly greater inDG ODE than in SG ODE. SG ODE tended tosupport higher NPP than did DG ODE. In addition,the groundwater depth strongly influenced spatial and temporal heterogeneity of NPP in thedesert ecosystems. Clearly, the desert ecosystemwith higher groundwater depth is more stable andmore resistant to long-term drought or climateshifts in arid regions. These investigations andquantitatively analysis are very significant for theexecution of conservation and restoration in aridecosystems.
Brown cotton is a kind of naturally colored cotton which accumulates brown pigment in fiber. In the experiment, the variations of DNA methylation and genes expression were studied during the development processes from 5 DPA to 25 DPA for brown fiber. By using the methylation-sensitive amplified polymorphism technique, we choose 66 pairs of selective-amplification primers to assess the status and levels of cytosine methylation. The hemimethylation of the external cytosine and the full methylation of the internal cytosine were scored. As a result, with the development of fiber from 5 DPA to 25 DPA, the ratios of the external hemimethylation and internal full methylation were gradually increased. In detail, at the stages of 5, 10, 15 and 25 DPA, the percentages of external hemimethylation were 8.29, 8.81, 9.77 and 10.09 %, the ratios of internal full methylation were 17.91, 19.20, 20.02 and 20.78 %, respectively. The development of brown fiber triggered the increase of methylated loci in the whole genome. For further analysis, we used cDNA-AFLP protocol to identify the specially expressed genes for further analysis. Totally, 30 polymorphic transcription-derived fragments (TDFs) were isolated and sequenced. By homologous alignment analysis, 19 TDFs were found similar with genes from cotton. Among them, the homologous genes of TDF5, TDF6, TDF10, TDF12, TDF17, TDF22, TDF23 and TDF25 were all from fiber. Furthermore, 13 TDFs were found to be homologous with reported functional genes.
G protein plays an important role in signaling pathways and is involved in various signal transduction systems in plant. A full-length cDNA encoding a putative G protein γ subunit (Gγ), designated as BnGG2, was isolated from Brassica napus L. BnGG2 is predicted to encode a precursor protein of 100 amino acid residues. The expression of BnGG2 in different B. napus tissues and developmental stages was analyzed by using real-time PCR. The results showed that BnGG2 expression level was high at the 7-day-old seedling stage, the bolting stage, and the fruiting stage. Moreover, BnGG2 was analyzed under four different plant hormones. All of the four tested hormones, abscisic acid (ABA), gibberellins acid 3 (GA₃), brassinosteroid (BR) and indole-3-acetic acid (IAA), triggered an induction of BnGG2 expression at different hormone concentrations. The expression of BnGG2 was significantly induced by the high concentrations of ABA, BR, and IAA. The expression of BnGG2 was also induced by low GA₃ concentrations and inhibited by high GA₃ concentrations. In addition, BnGG2 was responsive to different abiotic stresses. The BnGG2 was up-regulated in salt and drought stress and down-regulated in heat and cold stress. These results suggested that BnGG2 plays an important role in plant hormone signaling pathways and may be also involved in plant defense systems against environmental stresses in B. napus.
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