Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Cold tolerance of potato plants transformed with yeast invertase gene

100%

Deryabin A. N., Dubinina I. M., Burakhanova E. A., Astakhova N. V., Sabel'nikova E. P., Sinkevich M. S., Trunova T. I.

Acta Agrobotanica

2004

tom 57

nr 1-2

Our study was carried out with potato plants (Solanum tuberosum L., cv. Désirée) transformed with the yeast invertase gene under the control of the B33 c1ass I patatin promoter and with the proteinase inhibitor II leader peptide sequence praviding for the apoplastic enzyme localization (B33-inv plants) and with the plants transformed with the reporter gene encoding bb-glucuronidase under the control of the 35S CaMV promoter (control plants). Exposure to 5°C during 6 days caused an increase in invertase activity and sugar content in B33-inv leaves in comparison with the control plants. Cell membranes of B33-inv plant cells showed greater cold to1erance under low temperature conditions than control plants that was recorded by electrolyte release. We supposed that higher cold tolerance of B33-inv plants was caused by stabilizing effect of sugar on the membranes, because B33-inv plants differ from the control plants in higher invertase activity, induced by expression of yeast invertase gene, and high content of sugars.

Ograniczanie wyników

1 Acta Agrobotanica

1 Astakhova N. V.

1 Burakhanova E. A.

1 Deryabin A. N.

1 Dubinina I. M.

1 Sabel'nikova E. P.

1 Sinkevich M. S.

1 Trunova T. I.

1 2004

Wyniki wyszukiwania

Cold tolerance of potato plants transformed with yeast invertase gene