Wyniki wyszukiwania

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Hypoxic ventilatory profile in the anesthetized rat

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Marczak M., Kolesnikova E. E., Pokorski M.

Journal of Physiology and Pharmacology. Supplement

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2004

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tom 55

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nr 3

89-94

In the present study we investigated whether hypocapnia that accompanies hypoxic hyperventilation might affect the biphasic, stimulatory/depressant, ventilatory response to hypoxia. The experiments were carried out in anesthetized, vagotomized, spontaneously breathing, and poikilocapnic rats. The animals were subjected to acute steady-state hypoxia consisting of 12% O2 in N2 in inspiratory mixture. Ventilation and its frequency and volume components were assessed from the integrated electromyographic activity of the diaphragm. We found that despite the development of significant hypocapnia, the hypoxic ventilatory response consisted of rapid stimulation followed by a gradual decline. The frequency component contributed more to the ventilatory increase than that of volume. The results indicate that the hypoxic ventilatory profile in the anesthetized poikilocapnic rat resembles that known to be present during isocapnia. We conclude that hypocapnia neither hampers the hypoxic ventilatory reactivity nor alters the biphasic hypoxic ventilatory profile. These observations may aid planning experimental rat model studies.

2

Intermittent hypoxia: mechanisms of action and some applications to bronchial asthma treatment

100%

Serebrovskaya T. V., Swanson R. J., Kolesnikova E. E.

Journal of Physiology and Pharmacology. Supplement

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2003

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tom 54

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nr 1

35-41

Being essentially cut off from the global scientific community, Ukrainian and Russian scientists have developed a new concept for the beneficial use of adaptation to artificial intermittent hypoxia in treating of many diseases. The basic mechanisms underlying intermittent hypoxic training were elaborated mainly in three areas: regulation of respiration, free radical production and mitochondrial respiration. Twenty-year experience of the application of intermittent hypoxic therapy for the treatment of chronic obstructive bronchitis and bronchial asthma allows affirming that the adaptation to this kind of hypoxia causes a significant improvement of the clinical picture or even a complete recovery. The absence of negative side effects, typically observed during drug therapy, and the stimulation of organism’s general, nonspecific resistance, makes the hypoxic therapy a treatment with a future. A special note is devoted to the use of intermittent hypoxic training in industrial health care for the purpose of prophylaxis and treatment of professional diseases.

3

Age-related peculiarities of breathing regulation and antioxidant enzymes under intermittent hypoxic training

100%

Kolesnikova E. E., Safronowa O. S., Serebrovskaya T. V.

Journal of Physiology and Pharmacology. Supplement

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2003

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tom 54

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nr 1

20-24

Aging is associated with changes in breathing regulation, particularly, in respiratory sensitivity to hypoxic stimuli. One theory of aging holds that reactive oxygen species play a key role in this process. These species have also been implicated in the carotid body O2 sensing. In the present study we investigated hypoxic ventilatory responses (HVR) and antioxidant enzymes activity in healthy young and elderly people under a 14-day adaptation to intermittent hypoxic training (IHT). The elderly demonstrated decreased HVR and blood catalase (CAT) activity on a background of strong negative correlation between the levels of end-tidal CO2 tension and superoxide dismutase (SOD) activity. The adaptation to IHT resulted in increased HVR and SOD activity in both groups, and decreased CAT activity in young persons compared with its augmentation in the elderly. Increased SOD activity testifies to an overproduction of reactive oxygen species during IHT. We hypothesize that reactive oxygen species might potentiate a periodical augmentation of cytosolic Ca2+ in the carotid body. This, in turn, might determine an increase in tyrosine hydroxylase gene expression and promote neurotransmitter synthesis/release, resulting in enhanced HVR.

Ograniczanie wyników

3 Journal of Physiology and Pharmacology. Supplement

3 Kolesnikova E. E.

2 Serebrovskaya T. V.

1 Marczak M.

1 Pokorski M.

1 Safronowa O. S.

1 Swanson R. J.

1 2004

2 2003

Hypoxic ventilatory profile in the anesthetized rat

Intermittent hypoxia: mechanisms of action and some applications to bronchial asthma treatment

Age-related peculiarities of breathing regulation and antioxidant enzymes under intermittent hypoxic training