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We estimated the impact of seasonal and anthropogenic contaminants on antioxidant status and lipid peroxidation in common carp. The fish were reared in a power station cooling canal and in a natural pond. The examined blood parameters were: superoxide dismutase (0.91 to 5.01 U・mg⁻¹ HGB), glutathione peroxidase (3.13 to 21.03 U・mg⁻¹ HGB), bilirubin (0.44 to 1.22 mg・dl⁻¹), urea (1.66 to 6.33 mg・dl⁻¹), malondialdehyde (0.39 to 2.21 nmol・mg⁻¹ HGB), cadmium (0.031 to 0.088 mg・kg⁻¹ w.w.), and lead (0.035 to 0.131 mg・kg⁻¹ w.w.). Antioxidants were measured colorimetrically, and metals by atomic absorption. Fish living in cooling water had higher levels of all the parameters studied.
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The aim of the study has been to estimate the effect of culture conditions and a culture site on magnesium (Mg) concentrations in freshwater fish. The study encompassed tissues (blood) and organs (gills, liver, kidney, dorsal muscles) of five fish species: common carp (Cyprinus carpio L.), rainbow trout (Oncorhynchus mykiss Walbaum), Siberian sturgeon (Acipenser baeri Brandt), northern pike (Esox lucius L.) and grass carp (Ctenopharyngodon idella Valenciennes). A total of 125 fish comprised 25 individuals of each species, aged 6, 12, 18 and 24 months. The fish were cultured in privately owned fish breeding ponds (Western Pomerania, Poland). Tissue and organ samples were wet mineralised in concentrated HNO3 in a CEM MDS 2000 microwave oven. Magnesium concentrations were determined by inductively coupled plasma atomic emission spectrometry (ICP-MS) in a Jobin Yvon type JY-24 apparatus. The research had an approval of the Polish Local Ethics Committee no 9/05. The magnesium concentration in the tissues and organs ranged from 26.3÷174.2 mg kg–1 w.w. The lowest Mg concentration was found in the gills of rainbow trout (26.3±5.4 mg kg–1 w.w.), and the highest – in the liver of rainbow trout (174.2±27.6 mg kg–1 w.w.). The magnesium concentrations were also significantly affected by the type of feed.
The paper evaluates the effect of culture conditions and culture site on levels of certain microelements (Zn, Cu, Fe) and macroelements (Mg, Ca) in three species of freshwater fish: rainbow trout (Oncorhynchus mykiss Walbaum), common carp (Cyprinus carpio L.), and Siberian sturgeon (Acipenser baeri Brandt). The study involved 90 individuals of freshwater fish aged from 6 to 12 months. Samples of blood, liver, kidney, gills, skin and muscles were collected from each fish and subjected to chemical assay of Mg, Ca, Zn, Cu, Fe with inductively coupled plasma-atomic emission spectrometry in a JY-24 Jobin Yvon apparatus. The study revealed that culture site had statistically significant impact on levels of the examined elements among the three fish species. Mg content in kidney and skin was significantly higher in carp than in sturgeon. Similar regularities were observed for Ca content in skin and Zn content in kidney. Liver and kidney levels of Fe and Cu were significantly lower in carp than in rainbow trout. Of all the three examined fish species, rainbow trout had the highest skin levels of Ca and Mg, and the highest blood level of Fe. The results indicate that culture site and culture conditions exerted significant influence on levels of macro- and microelements in freshwater fish.
The aim of this study was to evaluate the content of lipids and their effect on progesterone concentration in the blood serum of cows. Experimental material was sampled from lactating and dry cows (41 specimen) of Polish Holstein-Friesian breed Black and White sub-species cattle, aged 3 to 6 years (lactation month 4-6 and pregnancy month 9, respectively) in two different maintenance periods, i.e. in summer and winter. In blood samples for analyses, triglycerides (TG), phospholipids (PL), total cholesterol (Cht) and its HDL fraction were determined using a Biomerieux reagent kits and a Kontron 92 UV-VIS spectrophotometer. LDL fraction of total cholesterol was calculated from the following equation: LDL [mmol/L] = Cht - HDL - TG/2.2. Progesterone concentration was determined by radioimmunoassay. The results were subject to statistical analysis by means of a Statistica 6.0 computer software package. Analysis of variance (Anova) was performed at significance from the external jugular vein in the morning hours. The blood serum concentrations at a level of p £ 0.05 were carried out. Pearson’s coefficient of correlation (r) was calculated to determine relationship between the examined lipids and progesterone. A significantly higher triglyceride concentration (0.32 mmol/L) was found in the blood serum of dry cows when compared to the lactating ones. The lactating cows had a higher content of phospholipids in both examined maintenance seasons. The content of total cholesterol and its HDL and LDL fractions was higher in both examined groups in summer. Blood serum progesterone concentration in both seasons was higher in the pregnant cows. Most of the positive dependencies between the concentration of studied lipids and progesterone concentration were found, especially in dry cows in winter.
The aim of the research was to determine the lipid profile in different age-groups of Simmental cattle. Studies were conducted on 90 specimens: 30 calves (up to 8-weeks-of-life), 30 heifers (up to 30-months-of-life), 30 adult cows (after 36-months-of-life). The following parameter levels were determined: total cholesterol, HDL, LDL, triglycerides and total lipids. The determined fraction levels in the majority were lower in calves. However, with heifers and adult cows they were comprised in the boundaries of the physiological norm or were slightly higher than it.
The aim of the study was to estimate the effect of culture conditions and culture site on magnesium (Mg) and zinc (Zn) concentrations in freshwater fish. The study encompassed dorsal muscles in five fish species: common carp (Cyprinus carpio L.), rainbow trout (Oncorhynchus mykiss Walbaum) and Siberian sturgeon (Acipenser baeri Brandt), northern pike (Esox lucius L.) and grass carp (Ctenopharyngodon idella Valenciennes). A total of 125 fish comprised 25 individuals of each species, aged from 6, 9, and 12 months. The fish were cultured in privately owned fish breeding ponds (Western Pomerania, Poland). For chemical and biochemical assays, samples of dorsal muscles were taken from each fish. Tissue samples were wet mineralised in concentrated HNO3 in a CEM MDS 2000 microwave oven. Magnesium and zinc concentrations were determined by inductively coupled plasma atomic emission spectrometry (ICP-MS) in a Jobin Yvon type JY-24 apparatus. The pursuit of the research we had an approval of the Polish Local Ethics Committee nr 9/05. The magnesium concentration in the dorsal muscles ranged from 95.3÷347.6 mg kg–1 w.w. The highest Mg concentration was found in rainbow trout (347.6±32.2 mg kg–1 w.w.), and the lowest in grass carp (95.3±11.3 mg kg–1 w.w.). Zinc concentration varied from 6.7÷98.8 mg kg–1 w.w. The highest was found in the muscles of Siberian sturgeon (98.8±0.4 mg kg–1 w.w.), and the lowest in rainbow trout (6.7±0.7 mg kg–1 w.w.). It was found that the breeding place significantly affected the Zn and Mg concentrations in the muscle tissue among individual freshwater fish species. The magnesium and zinc concentrations were also significantly affected by the type of feed.
This study aimed at determining relationships between the age of the Wielkopolski horses, ATP in whole blood and in the erythrocytes, and between erythrocyte Na+, K+-ATPase activity, and serum concentrations of mineral components. ATP was measured in whole blood and in erythrocytes by HPLC method. Serum concentrations of Ca²⁺ and Mg²⁺ were measured spectrophotometrically, while Na+ and K+ by flame photometry. In horses aged from 4 to 48 months, a dynamic decrease in ATP activity was found. Erythrocyte Na+, K+-ATPase activity decreased proportionally with the decrease in ATP activity. The results of this study may enable planning physical effort of horses at optimum use of energetic efficiency of their erythrocytes and mineral components in relation to their age.
Simmental is one of the two-direction breeds of milk-meat used due to its high growth values and muscularity. The aim of the study was to determine the level of hematological parameters in Simmental cattle from one herd of various age cattle by taking into consideration the period of pregnancy. Studies were conducted on 180 specimens: 90 cows at the age of between 3 to 8 years, 30 heifers, 35 calves and 25 pregnant cows, during the autumn. The following hematological parameters were determined: erythrocytes, leukocytes and platelets count, level of hemoglobin, haematocrit and red blood cell indicators. The analyzed level of basic hematological parameters corresponded with the cattle reference values. Red blood cell counts were lower but red blood cells indices values were increased. The values of hematological parameters in calves were lower in comparison with values noted in adult cows.
The aim of this study was to determine pig breed differences in semen parameters. A total of 1506 ejaculates were collected between 01.11.2005 and 31.04.2007 from 39 boars of WBP breed, PBZ breed and Pietrain-Duroc crossbreeds. All the ejaculates were taken and qualified for insemination at the Station of Sow Insemination in Świątki, the branch in Szczecinek, Poland. The following semen characteristics were examined: semen volume (after removing the gel plug) [ml], sperm concentration, live sperm count per ejaculate, percentage of live sperm with rapid or forward progression [%], sperm count in one insemination dose and the number of insemination doses received from a single ejaculate. The boar breed did not significantly influence the semen volume, sperm concentration, live sperm count, or the number of insemination doses from single ejaculates. Thus no clear effect of breed on semen characteristics has been determined.
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