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The aim of this study was to investigate of the consequent influence of auxins: IAA, IBA, NAA, used in the medium in vitro in the concentrations: 5, 10, 20, 40 μM, on the growth and development of Columnea hirta plants in the greenhouse. Microcuttings were planted in the sphagnum peat and coconut fibre. The planting was compared in two terms: spring and summer. Auxins used for the rooting of shoots in tissue culture had an influence on the capability for acclimatization and development of the microcuttings. The best quality of plants was observed when microcuttings were rooted in vitro on the medium containing 20 μM IBA, and then were cultured in the sphagnum peat during spring season.
Poznano wpływ następczy jaki wywierają auksyny stosowane na poziomie kultur sterylnych, na początkowy wzrost i rozwój mikrosadzonek Nematanthus x hybridus 'Tropicana' w warunkach szklarniowych. W etapie I - pędy ukorzeniano na pożywce MS (1962) z dodatkiem auksyn: IAA, IBA lub NAA w stężeniu: 5, 10, 20 lub 40 µM·dm⁻³. Kontrolę stanowiła pożywka bez auksyn. Po 21 dniach ukorzeniania przeprowadzono II etap doświadczenia. Mikrosadzonki posadzono w torfie wysokim i we włóknie kokosowym. Uprawę prowadzono w dwóch terminach - wiosennym i letnim. Po 8 tygodniach uprawy doświadczenie zakończono. Stwierdzono, że adaptacja mikrosadzonek, ich rozwój i wzrost zależały od auksyn stosowanych w kulturach in vitro na etapie ukorzeniania pędów. Rośliny Nematanthus X hybridus 'Tropicana' o najlepszej jakości otrzymano z mikrosadzonek ukorzenianych in vitro na pożywce zawierającej 20 i 40 µM IBA·dm⁻³, uprawianych w podłożu torfowym. Rośliny poddane in vitro działaniu NAA, uprawiane następnie w szklarni charakteryzowały się najniższą jakością.
Badano wpływ IAA, IBA i NAA w stężeniach: 5,0; 10,0; 20,0 i 40,0 µM·dm⁻³ na ukorzenianie pędów Nematanthus X hybridus 'Tropicana' in vitro. Kombinację kontrolną stanowiła pożywka podstawowa bez auksyn. Zastosowano pożywkę Murashige i Skooga [1962]. Auksyny wpływały istotnie na liczbę, wzrost wydłużeniowy oraz świeżą masę korzeni. Najlepszą rizogenezę (10,23-11,60 szt.) obserwowano na pożywkach zawierających 20,0-40,0 µM IBA·dm⁻³ lub 10,0 µM IAA·dm⁻³. Korzenie o największej długości i świeżej masie uzyskano w obecności 5,0 µM NAA·dm⁻³ (8,21 mm i 19,17 mg). Auksyna ta wpłynęła również na powstawanie tkanki kalusowej u podstawy pędów. Najlepszy wzrost mikrosadzonek obserwowano na pożywce zawierającej 40,0 µM IBA·dm⁻³.
The aim of this study was to investigate the influence of auxins and different their concentrations on rooting of Columnea hirta Klotzsch et Hanst. shoots in vitro. Shoots obtained from aseptically grown shoot clusters were cultured 3 weeks on Murashige and Skoog agar medium (MS) supplemented with: IAA, IBA or NAA in various concentrations: 5, 10, 20, 40 μM. Control cultures were incubated on MS medium devoid of any plant growth regulators. It was observed the significant influence of auxins on the growth of shoots, and the induction of roots. Microcuttings with the highest number of roots (11.24) were obtained on the media with 10 μM IBA, where mean length of root was 5.93 mm. The best elongation of roots was observed on the medium with 5 μM NAA (11.32 mm), but NAA caused regeneration of callus tissue at the base of shoots. Shoots presented the best growth and multiplication potential on the medium containing 10 μM IAA.
Mandevilla sanderi is an important commercial ornamental pot plant. Traditional vegetative propagation is limited due to the low rate, therefore there is a need to develop an alternative, more efficient method. There is an interest in development of micropropagation technology for the species, as it allows to obtain a lot of offsprings in a relatively short time. The aim of the present work was to estimate an influence of explants type and position on regeneration of Mandevilla sanderi in tissue culture. Four different types of explants (leafy shoot tips, decapitated leafy shoot tips, defoliated shoot tips, decapitated and defoliated shoot tips) were used in the experiment, which were placed on the media vertically, while defoliated shoot tips were placed horizontally or vertically upside down. The explants were cultivated on a Murashige and Skoog (MS) medium supplemented with 1 mg·dm–3 benzyladenine (BA) and 0.5 mg·dm–3 indole-3-butyric acid (IBA). It was noted that both explants orientation and positioning, influenced the multiplication rate. Defoliated shoot tips placed horizontally were characterized by higher multiplication rate (6.8) in comparison to upside down vertical positioning (3.2). It was also observed that removal of shoot apex improved axillary branching, while defoliation of shoots placed in a normal position reduced multiplication rate.
Garden perennials are a source of floristry material produced in field or under covers. A perennial producing long, leafy shoots, which in flower arrangements might successfully substitute fern or palm leaves, is Polygonatum multiflorum. The variety characterizing with decorative white margined leaves is ‘Variegatum’. To improve their quality, the growth stimulator Actisil Hydro Plus, which contains 0.6% of active silicon in a form of orthosilicic acid was applied. The aim of the conducted experiment was to estimate the influence of silicon and a place of cultivation (tunnel and open field) on post-harvest quality determined with morphological features, vase life longevity and mechanical properties of Polygonatum multiflorum (L.) All. ‘Variegatum’ stems. The plants were sprayed with water solutions of Actisil in concentrations: 0.2%, 0.3%, and 0.4%, six times during the growing season in weekly intervals, starting from the first decade of May, always in the morning. It was stated that cultivation of Polygonatum in the unheated foil tunnel increases quality of shoots for cut greenery, determined with morphological features and post-harvest longevity. Cultivation of Polygonatum in a foil tunnel and spraying plants with Actisil in concentrations of 0.3 or 0.4% is advantageous due to possibility to increase a number of shoots from 20% to over 31%, their length from 9% to 11% and fresh weight from 52% to 71%, in comparison to control plants. It is advised to treat plants cultivated in a tunnel, with Actisil in concentration of 0.4% as it improved shoots quality evaluated with their diameter and lower leaves blade area. Spraying plants with Actisil in concentrations of 0.2–0.4% in the tunnel and 0.2% in field prolongs post-harvest shoots longevity by 3–4 days. Plants cultivated in the tunnel characterize with higher strength of shoots determined with higher maximum force causing permanent damage and lower stem deflection than plants cultivated in field. Plants grown in a tunnel and sprayed with Actisil in concentrations of 0.4% characterized with the strongest shoots after 14 days of storage, however a similar effect was observed with the concentration of 0.3% and in case of plants cultivated in field and sprayed with 0.4% of Actisil.
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