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1

ZNF300, a recently identified human transcription factor, activates the human IL-2Rbeta promoter through the overlapping ZNF300/EGR1 binding site

100%
Xue L., Qiu H., Ma J., Guo M., Li W.
Cellular and Molecular Biology Letters
|
2010
|
tom 15
|
nr 4
ZNF300 was recently identified as a member of the human KRAB/C2H2 zinc finger protein family. Little is known about the role of ZNF300 in human gene regulation networks. In this study, the DNA-binding property of ZNF300 was further analyzed. We found that the recombinant ZNF300 could bind to the binding site 5′-GCGGGGGCG-3′ of Egr1, another member of the KRAB/C2H2 zinc finger protein family. Similarly, recombinant Egr1 also showed a similar binding affinity to the ZNF300 binding site 5′-CTGGGGGCG-3′. Bioinformatics analysis revealed that there is an overlapping ZNF300/Egr1 binding site in the human IL-2Rβ promoter region, which was previously known to be recognized by endogenous Egr1. Electrophoretic mobility shift assays showed that endogenous ZNF300 could also bind to this site. A transient transfection assay revealed that both ZNF300 and Egr1 could transactivate the IL-2Rβ promoter, and that the activation was abrogated by a mutation of residues in the overlapping ZNF300/Egr1 binding site. Co-expression of ZNF300 and Egr1 led to enhanced IL-2Rβ promoter activity. Thus, ZNF300 is likely to be another regulator of the human IL-2Rβ promoter.
2

Assessing nutrient elements as indicators for soil active organic carbon in topsoil of karst areas

86%
Qiu H., Liu J., Li L., Liu K., He X., Su Y.
Polish Journal of Environmental Studies
|
2019
|
tom 28
|
nr 3
In karst areas, in order to ascertain the relationship between soil active organic carbon fractions and soil nutrient elements, topsoil samples (the sampling points were set at 80-m intervals) were collected for the analysis of soil physicochemical properties. In the sampling area, land use was divided into upland, paddy, and shrub land. The results showed that dissolved organic C (DOC) and microbial biomass C (MBC) contents in shrub land soil were higher than those values in upland and paddy soils (p<0.05). The total nitrogen (TN) content in paddy was lower than in upland and shrub land (p<0.05). The mean value of total phosphorus (TP) in upland and shrub land was approximately 1.5 times higher than that in paddy. Available nitrogen (AN) in shrub land soil was higher than in upland, whereas Olsen-P was lowest in shrub land in all soils (p<0.05). The C:P and N:P ratios in upland and paddy were lower than in shrub land (p<0.05). At plot scale, random forest analysis revealed that pH and soil organic carbon (SOC) were the most important variables determining DOC content in croplands (upland and paddy) and shrub land, respectively. The accumulation of SOC accelerates the growth of microbial biomass in upland. In shrub land, increases in SOC and total nitrogen were in favor of microbial growth. At the small-watershed scale, C:P and N:P ratios had a significant and positive effect on the content of DOC and MBC, respectively. The results implied that high C and N availability, especially in combination with low P availability, is helpful for increasing soil microbial biomass.
3

Identification of the DNA binding element of the human ZNF300 protein

86%
Qiu H., Xue L., Gao L., Shao H., Wang D., Guo M., Li W.
Cellular and Molecular Biology Letters
|
2008
|
tom 13
|
nr 3
391-403
The human ZNF300 gene is a member of the KRAB/C2H2 zinc finger gene family, the members of which are known to be involved in various developmental and pathological processes. Here, we show that the ZNF300 gene encodes a 68-kDa nuclear protein that binds DNA in a sequence-specific manner. The ZNF300 DNA binding site, C(t/a)GGGGG(c/g)G, was defined via a random oligonucleotide selection assay, and the DNA binding site was further confirmed by electrophoretic mobility shift assays. A potential ZNF300 binding site was found in the promoter region of the human IL-2Rβ gene. The results of electrophoretic mobility shift assays indicated that ZNF300 bound to the ZNF300 binding site in the IL-2Rβ promoter in vitro. Transient co-transfection assays showed that ZNF300 could activate the IL-2Rβ promoter, and that the activation was abrogated by the mutation of residues in the ZNF300 binding site. Identifying the DNA binding site and characterizing the transcriptional regulation property of ZNF300 would provide critical insights into its potential as a transcriptional regulator.
4

The toxicity of cerium nitrate to Elodea canadensis: subcellular distribution, chemical forms and physiological effects

86%
Chu W. Y., Cai S. J., Fu Y. Y., Li F. F., Xu T., Qiu H., Xu Q. S.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 09
The rare earth elements are increasingly being used as trace supplements in different fields. In this study, subcellular distribution, the chemical forms and toxicity of cerium (Ce) were evaluated for Elodea canadensis. The effect of Ce (5–20 mg L⁻¹) applied for 7 days was assessed by measuring changes in the nutrient elements, photosynthetic pigments, malondialdehyde and antioxidant systems. Ce accumulation was greatest in the cell walls, followed by the organelles and the soluble fraction. Ce levels were higher in cellulose and pectin than in other biomacromolecules. The toxic effects caused by Ce were shown by a reduction in photosynthetic pigments, disruption of nutrient elements, and increases in MDA content. E. canadensis shows Ce-induced oxidative stress by modulating antioxidant enzymes, such as guaiacol peroxidase and catalase. Elevated Ce levels may represent a potential risk for aquatic ecosystems.
5

Characteristics of dissolved organic matter content in urban rivers under different environmental impact zones: a case study of China’s Tuo River

72%
Yu H., Gui H., Jiang Y., Li Z., Wang M., Fang H., Wang C., Chen C., Qiu H., Zhang Y.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 5
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