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1

Lilie jak dbać o jakość

100%
Chodorska M.
Owoce, Warzywa, Kwiaty
|
2011
|
nr 16
2

Jak wykrywać wirusy w krzewach borówki wysokiej?

84%
Chodorska M., Paduch-Cichal E.
Sad Nowoczesny
|
2011
|
tom [39]
|
nr 10
3

An improved method for RNA isolation from plants using commercial extraction kits

68%
Kalinowska E., Chodorska M., Paduch-Cichal E., Mroczkowska K.
Acta Biochimica Polonica
|
2012
|
tom 59
|
nr 3
Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. This study presents a simple and efficient modification of RNA extraction from different types of tissues using two commercial reagent kits. By simple improvement, we routinely obtained high-quality RNA of the following plants: the blackcurrant bush, black chokeberry bush, pear tree, apricot tree, apple tree, hardy kiwi, tangerine tree, highbush blueberry and cranberry plant.
4

Wirusy - zagrożenie dla upraw czosnku w Polsce

68%
Chodorska M., Paduch-Cichal E., Kalinowska E., Szyndel M. S.
Warzywa
|
2014
|
nr 06
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Assessment of allexiviruses infection in garlic plants in Poland

68%
Chodorska M., Paduch-Cichal E., Kalinowska E., Szyndel M. S.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2014
|
tom 13
|
nr 2
Garlic (Allium sativum L.) plants may be infected in the field by viruses of the genera Potyvirus, Carlavirus and Allexivirus. These viruses are transmitted by vegetative propagation and by vectors. Detection and identification of allexiviruses was carried out in 2011–2012. It was based on ELISA and RT-PCR assays. Samples of plant material were collected from 26 garlic production fields located in different regions of Poland. Garlic virus D, Garlic virus B and Garlic virus X were the most abundant viruses in all examined regions and were identified in 79%, 64% and 59% of all garlic samples, respectively. Garlic virus A and Garlic virus C were identified in all studied regions with low frequency. Garlic virus E was detected with 100% frequency in east-central Poland. None of the tested garlic samples were infected with Shallot virus X. Allexiviruses were always present in garlic plants in mixed infections.
6

Occurrence of the viruses belonging to the Allexivirus genus on garlic plants in Poland

68%
Chodorska M., Paduch-Cichal E., Kalinowska E., Szyndel M. S.
Progress in Plant Protection
|
2013
|
tom 53
|
nr 3
Garlic (Allium sativum L.) can be infected by numerous viruses that are spread by the seed bulbs during the vegetative propagation of the plants. Eight garlic viruses that belong to the Allexivirus genus are transmitted by mites. The aim of this study was to detect and identify Allexiviruses infecting garlic in Poland. Identification of Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and Shallot virus X (ShVX) was based on ELISA (Enzyme-Linked Immunosorbent Assay) and confirmed by RT-PCR (Reverse Transcription Polymerase Chain Reaction). Garlic virus D (GarV-D), Garlic virus E (GarV-E) and Garlic virus X (GarV-X) were detected using RT-PCR. The samples of garlic plants were collected in September 2011 and July 2012 from garlic production field located in different part of Poland. Garlic virus D, Garlic virus X and Garlic virus B were the most abundant garlic viruses in all examined regions and were identified in 84, 67 and 54% of all samples, respectively. Garlic virus A and Garlic virus C were detected in all studied regions with low frequency. None of the tested garlic samples were infected with Shallot virus X. Allexiviruses that were present in garlic plants occurred always in mixed infections.
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The effect of growth regulators and preservative on senescence of cut oriental lily 'Helvetia'

68%
Rabiza-Swider J., Skutnik E., Chodorska M.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2012
|
tom 11
|
nr 5
The quality of cut lily flowers during its postharvest life is important both for growers and customers. It is determined by two factors: longevity of flowers and a general appearance of the entire cut stem during its vase life. The aim of this work was to evaluate the effects of growth regulators (gibberellic acid and benzyladenine) and the preservative (200 mg·dm⁻³ 8HQC+2%S) on quality of cut oriental lily ‘Helvetia,’ a white blooming cultivar very popular on the Polish market. After cutting the flowers were treated for 20 h with growth regulators (GA3 or BA, 500 mg·dm⁻³) and then placed into distilled water. Non pulsed flowers were kept in a preservative solution composed of 200 mg·dm⁻³ 8HQC and 2% sucrose. Untreated flowers held in distilled water served as a control. During the senescence of cut lily flowers the soluble protein and free proline contents were determined. As the major problem in the postharvest handling of lilies is leaf yellowing, the effects of postharvest treatments on quality of leaves and their chlorophyll contents were also studied. The preservative increased vase life of cut lily flowers while gibberellic acid improved the quality of leaves. In leaves treated with GA3 the chlorophyll level was 25% higher than in leaves on stems placed into distilled water directly after harvest. The level of soluble protein in petals dropped while free proline accumulated during flower senescence. Flowers treated with GA3 and those placed into 8HQC + 2%S solution showed a delayed protein degradation: the concentrations of soluble proteins after 12 day of vase life was over 3-fold higher than in control flowers. Also the proline accumulation was delayed in flowers on stems placed in the preservative solution, however, no effect of GA3 on the proline level was observed. The soluble protein level correlated with a flower position on the stem being lower in lower flowers as compared to the upper ones.
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Year-round blueberry scorch virus detection in highbush blueberry

68%
Paduch-Cichal E., Chodorska M., Kalinowska E., Komorowska B.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2014
|
tom 13
|
nr 3
Viral diseases are a worldwide problem of blueberry which a major limiting factor for production. A survey for Blueberry scorch virus (BlScV) by DAS-ELISA in various organs of highbush blueberry conducted from May 2010 to April 2011, showed the occurrence of these virus in cvs Bluecrop and Herbert, which showing virus-like symptoms. Samples of plant materials (bud flower, flower, leaf, bark) were collected individually from each highbush blueberry plant of every cultivar. It was established that the detection of virus of each the investigated bushes cvs Bluecrop and Herbert depended on the tested plant materials as well as the period in which the tests were performed. The effectiveness of the virus detection varied for the investigated cultivars. The presence of the BlScV was confirmed in leaves samples with specific primer pair which amplifies a 430 bp fragment of the 5’-proximal ORF I [RNA-dependent RNA polymerase (RdRp)].
9

Choroby wirusowe na plantacjach borówki

68%
Paduch-Cichal E., Kalinowska E., Chodorska M.
Hasło Ogrodnicze
|
2011
|
nr 03
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Detection and identification of viruses of highbush blueberry and cranberry using serological ELISA test and PCR technique

60%
Paduch-Cichal E., Kalinowska E., Chodorska M., Sala-Rejczak K., Nowak B.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2011
|
tom 10
|
nr 4
The problems in the cultivation of highbush blueberry and cranberry are diseases caused by infections factor, particularly by fungi and lately also by viruses. In the years 2008–2010 research concerning the detection and identification of viruses occurring on production plantations of the highbush blueberry located in the central and southeastern Poland and the cranberry growing on the separate parts of the plantation in the central Poland using the serological ELISA test and PCR technique were performed. The results of the performed serological ELISA test showed the presence on the bushes of various cultivars of the Blueberry shoestring virus (BSSV) and Peach rosette mosaic virus (PRMV) (central and south-eastern Poland) and the Blueberry scorch virus (BlScV) and Tobacco ringspot virus (TRSV) (central Poland). During the observations carried out on the plantings of the highbush blueberry only the symptoms characteristic for the infection with the BlScV were noted (central Poland). This virus was also detected using DASELISA test in the cranberry plants growing in the separate parts of plantations in the central region of Poland (Plantation A/W), which did not show any disease symptoms. In Europe it is the first report on the occurrence of BlScV in the cranberry bushes. What is more, it was established that the viruses can be detected in the leaves, the flowers and the phloem + periderm + cortex parenchyma samples in which the investigations could be performed in various months in the year. In the bushes of the blueberry of the Darrow and Herbert cultivars from Plantation A/W (central Poland) showing the symptoms in the form of red spots on the leaves or red spots and rings on the stems the presence of the Blueberry red ringspot virus (BRRSV) was confirmed using the PCR technique. In Poland it is the first report concerning the occurrence of the virus in the bushes of the highbush blueberry following those published in the Czech Republic and Slovakia.
11

Wykrywanie i identyfikacja wirusów borówki wysokiej przy użyciu: testu serologicznego ELISA, technik elektronomikroskopowych oraz technik biologii molekularnej

51%
Paduch-Cichal E., Kalinowska E., Sala-Rejczak K., Chodorska M., Nowak B., Gajewski Z., Tomala K.
Progress in Plant Protection
|
2011
|
tom 51
|
nr 1
The aim of this work was the detection and identification of blueberry viruses in different blueberry cultivars grown in the central and south-east parts of Poland. The plant materials were analyzed by serological ELISA test. Blueberry scorch virus (BlScV), Blueberry shoestring virus (BSSV), Peach rosette mosaic virus (PRMV) and Tobacco ringspot virus (TRSV) were detected. Bundles of viruslike particles were observed in cytoplasm of BlScV infected material. The size of the particles was about 85 nm or more in length × 14 nm in diameter. The primer set RRSV3/RRSV4, which amplifies the fragment of the transcriptional activator gene was used in PCR for BRRSV detection. Sequencing analysis of the amplified products revealed 93–96% nucleotide sequence homology with the BRRSV-NJ putative transcriptional activator gene from the GenBank (Accession No. AF404509).
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