The aim of the study was to find the direct effects of a possible correlation between different glutamate concentrations (5, 50 and 200 µM) on the one hand and catecholamine release from rabbit hypothalamus homogenate during a 90 min incubation on the other hand. Brain structures were obtained from 12 decapitated female rabbits that were 12 weeks old. After decapitation the obtained brain of the animal was placed in 0.9% NaCl. Next, each brain structure from each rabbit (approx. 50 mg) was placed in incubation wells (cell culture Sigma) containing 1 ml of incubation medium (Krebs phosphate buffer containing glucose 0.3% bovine serum albumin and 0.1% BSA) without (control) or with three doses of Glu in a concentration of the following: I – 5 μM, II – 50 μM, and III – 200 μM. Every 30 minutes each slice of tissue of each brain structure was placed in the next hole with the medium. The medium collected after 30, 60 and 90 minutes of the experiment was used for dopamine, noradrenaline and adrenaline RIA measurement. The obtained results show that Glu has a direct influence on catecholamines release from the hypothalamus as a central brain structure of the HPA axis in stress inducing and adaptive processes. Generally, there was an inhibitory effect on catecholamine release from the hypothalamus homogenates. Our findings show that Glu has great influence on the brain’s motivational structure, which may indicate its contribution to stress response by modulating the amount of catecholamine released.
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