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1

Expression of recombinant porcine lactoferrin N-lobe in Pichia methanolica and its antibacterial activity

100%
Shan T. Z., Wang Y. Z., Liu G. F., Huang H. Q.
Journal of Animal and Feed Sciences
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2007
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tom 16
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nr 2
283-292
2

Postnatal expression pattern of the C-EBP alpha gene in porcine subcutaneous adipose tissue

88%
Jiang J. F., Xu Z. R., Wang Y. Z., Han X. Y., Wang L.
Journal of Animal and Feed Sciences
|
2006
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tom 15
|
nr 1
61-70
CCAAT/enhancer binding protein α (C/EBPα) has been implicated as a key regulator of adipocyte differentiation and lipid metabolism. The pattern of C/EBPα gene expression in different growth stages and its relation to adipose deposition was studied. Fifteen female Duroc × Landrace × Yorkshire pigs in five groups of three pigs each, weighing 1, 30, 50, 70 and 90 kg were used to study developmental gene expression of C/EBPα in subcutaneous adipose tissue by means of semi-quantitative RT-PCR. The results showed that the C/EBPα mRNA levels in porcine adipose tissue continuously increased as the pigs grew and deposited fat from 1 to 90 kg body weight (BW) (P<0.05). The presented data show a close positive correlation between the levels of C/EBPα gene expression and the fat deposition rate in pigs. The experiment showed that total adipose weight increased significantly with BW (P<0.05).
3

Effect of dietary bovine lactoferrin on lipid peroxidation and activities, and mRNA levels of antioxidant enzymes of piglets

88%
Xu C. L., Wang Y. Z., Liu J. X., Yao G. J., Feng J.
Journal of Animal and Feed Sciences
|
2006
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tom 15
|
nr 4
609-620
The experiment was conducted to evaluate effect of dietary bovine lactoferrin (bLF) on lipid peroxidation and activities as well as mRNA levels of antioxidant enzymes of piglets. Duroc×Landrace×Yorkshire crossbred female piglets (n=120, 35 days of age, liveweight 9.70±0.71 kg) were fed a diet containing 0, 1250, or 2500 mg/kg bLF for 30 days. After completion of the feeding experiment, twelve female piglets with 4 animals in each treatment were randomly selected to determine malondialdehyde (MDA) and total antioxidant capacity (TAOC) levelsczinc- superoxide dismutase (CuZnSOD), glutathione perioxidase (GPx), catalase (CAT) activities in serum and liver, and CuZnSOD, GPx and CAT mRNA levels in liver. Results showed that piglets treated with 2500 mg/kg bLF significantly increased (P<0.05) TAOC levels, the activities of GPx, CuZnSOD and CAT, and mRNA levels of CuZnSOD, GPx and CAT, and decreased (P<0.05) the contents of MDA as compared with control. Supplementation with 1250 mg/kg bLF also increased (P<0.05) the activities of CuZnSOD, GPx and CAT and mRNA levels of GPx and CAT, and decreased (P<0.05) the contents of MDA as compared with control, but the effect was not better than that of dietary addition of 2500 mg/kg bLF (P<0.05). The study indicated that addition of bLF improved the antioxidant function of piglets by up-regulation of mRNA levels and activities of certain antioxidant enzymes associated with free radicals metabolism.
4

Developmental gene expression of antimicrobial peptide Protegrin-1 and effect of weaning on gene regulation of Protegrin-1 in piglets

88%
Han F. F., Wang Y. Z., Feng J., Guo J., Xu Z. R.
Journal of Animal and Feed Sciences
|
2007
|
tom 16
|
nr 1
86-95
5

Effect of dried roots of Astragalus membranaceus in the diets of young growing pigs on growth performance and immune function

76%
Hu Y. L., Xu C. L., Wang Y. Z., Li Y. J., Liu J. X., Feng J.
Journal of Animal and Feed Sciences
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2006
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tom 15
|
nr 4
599-607
The experiment was conducted to investigate the effect of Astragalus powder prepared by using different comminution techniques on growth performance and immune function of pigs. Duroc×Landrace×Yorkshire young growing pigs (n=90, female, 60 days of age, liveweight 21.88±1.26 kg) were randomly allocated to three treatments. Each treatment had three replicates with ten pigs per pen. The basal diets were not supplemented or supplemented (5 g/kg) with 80 mesh Astragalus (180 μm), or micron Astragalus (6.32 μm), respectively; the feeding experiment lasted 30 days. After completion of the feeding experiment, three animals from each treatment were chosen to determine the effect of Astragalus on immune function. Results showed that supplementation with micron Astragalus significantly increased (P<0.05) average daily gain (ADG), both Concanavalin (ConA) and lipopolysaccharide (LPS)-induced splenocyte and peripheral lymphocyte proliferation, and significantly increased (P<0.05) serum IgG, IgA, IL-1α and IL-2 concentrations compared with the control groups. Supplementation with 80 mesh Astragalus only increased (P<0.05) serum IgA, IL-1α and IL-2 concentrations compared with control. The results indicated that micron Astragalus was more effective than 80 mesh Astragalus in improving growth performance and enhancing immune function of pigs.
6

Developmental expression of the lipoprotein lipase gene in porcine subcutaneous adipose tissue

76%
Shan T. Z., Wang Y. Z., Liu Y. J., Liu J. X., Feng J., Xu Z. R.
Journal of Animal and Feed Sciences
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2006
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tom 15
|
nr 4
621-629
Lipoprotein lipase (LPL) has been implicated as a key enzyme in lipid metabolism. The pattern of LPL gene expression in different ages and its relation to adipose deposition was studied. Fifteen female Duroc × Landrace × Yorkshire pigs aged at 1 day, 7, 14, 21 and 28 weeks (three pigs per age, 10% per each bred) were used to study developmental gene expression of LPL in subcutaneous adipose tissue by means of semi-quantitative RT-PCR. The results showed that the LPL mRNA levels in subcutaneous adipose tissue significantly increased from 1 day to 7 weeks (P<0.01), and then gradually decreased from 7 to 28 weeks. A strong inverse correlation between LPL mRNA levels and age as well as adipose index and body weight was found between 7 and 28 weeks. Body weight and total adipose weight increased significantly with age (P<0.01).
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