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Gene expression in chilling sensitive and resistant lines of maize under low temperature treatment

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Mazza R., Bertoli A., Pasini L., Marocco A.

Zeszyty Problemowe Postępów Nauk Rolniczych

2006

tom 509

The chilling-susceptible virescent-2 and the tolerant Oh43 strains were used as a tool to identify genes involved in the response to low-temperature conditions. Plants were grown in a controlled environment chamber at 15°C under continuous light. Seedlings of maize exhibited chlorosis when grown at 15°C. Chlorotic leaves showed severely reduced level of photosynthetic pigments and the rate of photosynthesis. The cDNA-AFLP-TP technique was employed in order to identify the differentially expressed genes. This technique allows to obtain a unique restriction fragment from each cDNA. Application of the optimised protocol for isolating, reamplifying, cloning and sequencing transcript-derived fragments, allowed the identification of 329 sequences which were related to the virescent temperature-sensitive events. Most of the sequences, analysed with bioinformatic tools, showed a consistent homology with database entries and they were classified in several functional groups. Certain sequences showed similarities to ESTs isolated in cold-stressed plants. The gene list represents a potential source for genes with a critical role in plant acclimation to suboptimal temperatures.

Ograniczanie wyników

1 Zeszyty Problemowe Postępów Nauk Rolniczych

1 Bertoli A.

1 Marocco A.

1 Mazza R.

1 Pasini L.

1 2006

Wyniki wyszukiwania

Gene expression in chilling sensitive and resistant lines of maize under low temperature treatment