11 polysaccharide fractions were isolated from the Echinacea purpurea herb (collected from the cultivation) and 8 compounds were isolated from the callus tissue from in vitro cultures. The HPLC analysis of hydrolysed polysaccharides indicated a great heterogeneity of the herb fractions. They differ significantly from the compounds obtained from the callus tissue, which are mostly arabinogalactans with an addition of xylose.