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Molecular detection of crimean-congo hemorrhagic fever virus (CCHFV)in tick samples but not in blood and milk samples of domestic ruminant species (cattle, sheep and goat) in northern Turkey

100%
Ozupak T., Albayrak H.
Polish Journal of Veterinary Sciences
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2020
|
tom 23
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nr 4
2

Rotavirus and coronavirus prevalence in healthy calves and calves with diarrhoea

81%
Okur G. S., Yazici Z., Albayrak H., Meral Y.
Medycyna Weterynaryjna
|
2007
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tom 63
|
nr 01
62-64
The aim of the study was to examine clinical and subclinical BRV and BCV infection rates in calves and serologic prevalance of cows in northern Turkey. In this study, 100 fecal specimens collected from healthy calves (0-3-months-old) and calves with diarrhoea were examined for bovine rotavirus (BRV) and bovine coronavirus (BCV) antigens, with 23% and 1% positivity detected respectively. BRV antigen positivity was 4.08% in healthy calves but BCV antigen was not detected. BRV and BCV antigen positivity was 41.17% and 1.96% in calves with diarrhoea. Antibody titers against BRV and BCV in 256 adult cows sampled in the same region were 71.48% and 98.43%, respectively. As the result, it is concluded that BRV-sourced infections in calves with diarrhoea are frequently encountered (41.17%), but BCV infections are rarely detected (4.08%). In contrast, BCV infection in cows are more frequently encountered than BRV infections in Northern Turkey. Data from this study reflects the widespread distribution of BRV infections in 0-3-month-old animals and BCV infections in animals older than 3 months.
3

Serological profile of some viral infections in unvaccinated cattle in Turkey

81%
Yazici Z., Okur Gumusova S., Albayrak H.
Medycyna Weterynaryjna
|
2007
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tom 63
|
nr 02
187-189
In this study, 936 unvaccinated cattle sera were analyzed against five viruses: bovine viral diarrhea virus (BVDV), parainfluenzavirus type 3 (PIV-3), bovine adenovirus type 1 (BAdV-1), bovine adenovirus type 2 (BAdV-2) and bovine adenovirus type 3 (BAdV-3), which were caused by respiratory system diseases in cattle. The study used the serum neutralization (SN) test - a conventional method. The seropositive rates for each virus were found to be 20.19%, 41.02%, 23.82%, 21.90% and 8.1%, respectively. Evaluation of the data revealed that 25.64% of the sampled population was seronegative against the investigated viruses, 74.36% were seropositive for one or more of them and 42% of animals were found to be seropositive against any of the investigated viruses. 25.42% of the animals were seropositive against two viral infections whereas 5.23% of the animals were found to be seropositive against three viral infections and 0.1% of the animals were detected to be seropositive for four viruses.
4

Molecular detection and seasonal distribution of lumpy skin disease virus in cattle breeds in Turkey

61%
Albayrak H., Ozan E., Kadi H., Cavunt A., Tamer C., Tutuncu M.
Medycyna Weterynaryjna
|
2018
|
tom 74
|
nr 03
The aim of this study was to investigate the prevalence and distribution patterns of LSDV infections in the provinces of northern Turkey, and to detect the factors influencing the epidemiology of LSD virus infections (age, breed, season, climate, geography, population dynamic, animal movement), as well as to assess the diagnostic value of the sampled materials in the diagnosis of LSDV infections. Lumpy skin disease (LSD) is an economically important cattle disease. The disease is endemic in many African countries, but outbreaks have also been reported in Turkey and the Middle East. In this study, a total of 564 samples (skin, blood and lung) from different cattle breeds (Jersey, Holstein-Friesian, Anatolian Black, Simmental and Brown Swiss) (n = 465) in the many herds suspected of lumpy skin disease virus (LSDV) infection as clinically and macroscopic pathologic remarks, housed in the 7 different provinces of northern Turkey, were used for gel based conventional polymerase chain reaction (PCR). LSDV nucleic acid was detected in 259 of 564 (45.92%) materials by PCR. According to the result of PCR, the LSDV infection was diagnosed in 54.62% (254/465) of the sampled animals. The diagnostic value of necropsy and clinical materials such as skin and lung were determined as more valuable diagnostic materials in the diagnosis of LSDV infection by PCR. Data showed that LSDV infection was widespread in the provinces of northern Turkey and that the prevalence of the infection in the region varies in accordance with factors such as geographical conditions (climate, season, location etc.) and the method of breeding.
5

Molecular identification and phylogenetic analysis of non-cytopathic bovine viral diarrhea virus isolate obtained from a local respiratory outbreak in Northern Turkey

51%
Yazici Z., Gumusova S., Tamer C., Cagirgan A. A., Akman A., Ozan E., Kadi H., Palanci H. S., Albayrak H.
Medycyna Weterynaryjna
|
2017
|
tom 73
|
nr 05
In February 2016, a local respiratory disease outbreak with two fatalities was reported in Samsun, Turkey. A non-cytopathic bovine viral diarrhea virus (ncp-BVDV) was identified from the organ and leucocyte samples of dead juvenile heifers using RT-PCR with specific primers for the NS2/3 gene coding region. The NS2/3 gene of BVDV was sequenced and compared with other published sequences of BVDV. The sequences of our isolate which was named as Samsun TR, had 81-83% nucleotite (nt) identity for BVDV-1. Phylogenetic analysis revealed that Samsun-TR was closely related to LC089875 (Japan), AF526381 (China) and also shared 83% nucleotide(nt) identity with them. The NS2/3 gene sequence of Samsun-TR was deposited in the GenBank database with the accession number of KX428495.
6

Molecular identification and phylogeny of bovine herpesvirus-1 (BoHV-1) from cattle associated with respiratory disorders and death in Turkey

42%
Albayrak H., Tamer C., Ozan E., Muftuoglu B., Kadi H., Dogan F., Akman A., Cagirgan A. A., Elhag A. E., Kurucay H. N., Gumusova S., Yazici Z.
Medycyna Weterynaryjna
|
2020
|
tom 76
|
nr 06
This study aimed to investigate the presence of bovine herpesvirus 1 by molecular techniques in two cases of respiratory disease in beef cattle, reported from Amasya Province of Turkey in 2018. Nasal swab and lung tissue samples were taken. The presence of bovine herpesvirus-1 (BoHV-1) was confirmed by the PCR method using glycoprotein B (gB) gene-specific primers, and then the isolates were also subjected to partial sequencing. The results of the phylogenetic analysis revealed that the two new isolates in Turkey belonged to the same subclade as subtype 1.1 of BoHV-1, and both also had a 100% nucleotide (nt) homology with the Cooper reference strain of BoHV-1. These findings can enrich the gB sequence content data for BoHV-1 found in GenBank regarding Turkey.
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