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A new anti-counterfeiting method: fluorescent labeling by safranine T in tobacco seed

100%

Guan Y., Hu J., Li Y., Ma W., Zheng Y.

Acta Physiologiae Plantarum

2011

tom 33

nr 4

Counterfeit seeds cause a large loss in seed industry and crop production. Present study was undertaken to find a new anti-counterfeiting technology by labeling seeds with a fluorescent compound, safranine T (ST). Response surface analysis (RSA) experiment with two factors (ST concentration and seed soaking time) of 14 combinations was performed to investigate the ST effects on seed vigor and fluorescence showing in seedlings of two tobacco varieties, MS Yunyan 85 and Honghua Dajinyuan. After soaking in ST solutions, tobacco seeds were germinated for 16 days, then the germination index (GI), vigor index (VI), length of roots and shoots were determined. The optimum combinations of ST concentration and soaking time on GI, VI and length of shoots and roots were 0.57–0.69 mg/ml and 6.77–9.34 h for MS Yunyan 85 and 0.51–0.80 mg/ml and 5.81–7.39 h for Honghua Dajinyuan, respectively. Meanwhile, tobacco seedlings, treated with ST solutions from 0.3 to 1.5 mg/ml showed obviously fluorescence under illumination of green light. It suggests that soaking tobacco seeds in safranine T solution of suitable concentration can be used as a labeling technique for anti-counterfeiting in tobacco seeds.

Effects of mismatches and insertions on discrimination accuracy of nucleic acid probes

100%

Piao X., Sun L., Zhang T., Gan Y., Guan Y.

Acta Biochimica Polonica

2008

tom 55

nr 4

713-720

Effective discrimination of non-complementary nucleotides is an important factor to ensure the accuracy of hybridization-based nucleic acid analyses. The current study investigates the effects of the chemical nature, the positions, the numbers, and the cooperative behavior of mismatches as well as insertions on 20-mer and 30-mer duplexes. We observed the hybridization stability trend affected by mismatches: G:T ≈ G:G > G:A > A:A ≈ T:T > A:C ≈ T:C > C:C. The experimental data show that mismatches at the center of the oligonucleotide probes have a more profound destabilizing effect on the hybridization stability than those at either ends. Insertions also demonstrate a similar destabilizing effect as mismatches. These results provide useful information for designing DNA microarray nucleotide probes and for improving the discrimination accuracy of hybridization-based detections.

Comparative transcriptome analysis reveals the responses of winter rye to cold stress

86%

Kong Y., Zhang T., Guan Y., Wang L., Gu X., Yang S.

Acta Physiologiae Plantarum

2020

tom 42

nr 05

Ograniczanie wyników

2 Acta Physiologiae Plantarum

1 Acta Biochimica Polonica

3 Guan Y.

2 Zhang T.

1 Gan Y.

1 Gu X.

1 Hu J.

1 Kong Y.

1 Li Y.

1 Ma W.

1 Piao X.

1 Sun L.

1 Wang L.

1 Yang S.

1 Zheng Y.

1 2020

1 2011

1 2008

Wyniki wyszukiwania

A new anti-counterfeiting method: fluorescent labeling by safranine T in tobacco seed

Effects of mismatches and insertions on discrimination accuracy of nucleic acid probes

Comparative transcriptome analysis reveals the responses of winter rye to cold stress