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Novel SNP of the goat prolactin gene [PRL] associated with cashmere traits

100%

Lan X.-Y., Pan C.-Y., Chen H., Lei C.-Z., Li F.-Y., Zhang H.-Y., Ni Y.-S.

Journal of Applied Genetics

2009

tom 50

nr 1

51-54

Concentrations of the single-chain polypeptide hormone prolactin (PRL) are associated with wool or cashmere traits, and its seasonal changes may determine patterns of enzymatic activity and may affect cashmere fibre growth and moult. So, the PRL gene is a potential candidate gene for cashmere traits in marker-assisted selection (MAS). In this paper, we report a novel missense single-nucleotide polymorphism (SNP) within the goat PRL gene in 1367 individuals by PCR-SSCP (polymerase chain reaction with single-strand conformation polymorphism) analysis and DNA sequencing. The novel X76049:g.576C>A mutation is confirmed by Eco241 PCR-RFLP (restriction fragment length polymorphism) analysis and causes a missense codon (Pro176Thr). The frequencies of allele C varied from 0.79 to 0.93 in 9 analysed goat populations. C allele was correlated with higher fibre length (P = 0.014).

Novel 12-bp deletion in the coding region of the bovine NPM1 gene affects growth traits

68%

Huang Y.-Z., Zhang E.-P., Chen H., Wang J., Li Z.-J., Huai Y.-T., Ma L., Lan X.-Y., Ren G., Lei C.-Z., Fang X.-T., Wang J.-Q.

Journal of Applied Genetics

2010

tom 51

nr 2

199-202

The nucleophosmin 1 gene (NPM1) encodes a multifunctional nucleolar phosphoprotein that plays a crucial role in the control of various aspects of cell growth and homeostasis. In this study, the coding region of the NPM1 gene was screened in 1035 individuals of 4 Chinese cattle breeds by DNA sequencing and Polyacrylamide gel electrophoresis. A novel 12-bp deletion mutation was identified in the coding region of the NPM1 gene. The PCR products of primer NPM1-P2 exhibited 3 genotypes and 2 alleles: 178 bp (denoted as W) and 166 bp (denoted as D). Genotype DD and allele D were predominant in the studied populations. Association analysis with growth traits in the Nanyang breed (N = 265) showed that the animals with genotype DD had significantly greater birth weight, body weight, body length, and heart girth than those with genotype WD (P <0.01 or P < 0.05) at birth and after 6 months and 12 months, but not at 18 and 24 months of age. Results of this study suggest that the NPM1 gene is a candidate gene for growth traits in cattle.

Ograniczanie wyników

2 Journal of Applied Genetics

2 Chen H.

2 Lan X.-Y.

2 Lei C.-Z.

1 Fang X.-T.

1 Huai Y.-T.

1 Huang Y.-Z.

1 Li F.-Y.

1 Li Z.-J.

1 Ma L.

1 Ni Y.-S.

1 Pan C.-Y.

1 Ren G.

1 Wang J.

1 Wang J.-Q.

1 Zhang E.-P.

1 Zhang H.-Y.

1 2010

1 2009

Wyniki wyszukiwania

Novel SNP of the goat prolactin gene [PRL] associated with cashmere traits

Novel 12-bp deletion in the coding region of the bovine NPM1 gene affects growth traits