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Silicon (Si) has been verified to play an important role in enhancing plant resistance against pathogens, but the exact mechanisms remain unclear. Two near-isogenic lines of rice (Oryza sativa L.), CO39 (blast susceptible), and C101LAC (Pi-1) (blast resistant), were hydroponically grown to study the effects of exogenous silicon application on the changes of disease incidence, mineral nutrient concentrations, chlorophyll content, and photochemical efficiency in Magnaporthe oryzae infected rice plants. Si amendment in nutrient solution at a concentration of 2.0 mM significantly reduced the disease index of rice plants of CO39 and C101LAC (Pi-1). Silicon application alone had no effects on mineral nutrient contents, chlorophyll content, maximum/potential quantum efficiency (Fv/Fm), and the maximum primary yield (Fv/F0) of photochemistry of PS II in healthy rice leaves. M. oryzae inoculation significantly increased the content of K, Na, Ca, Mg, Fe, and reduced the value of Fv/F0 and Fv/Fm in rice leaves. However, Si treatment suppressed M. oryzae induced increase of mineral nutrient contents, and significantly increased Fv/F0 and Fv/Fm value compared with Sideficient infected plants. These results suggest that siliconenhanced resistance to rice blast is associated with an enhancement of photochemical efficiency and adjustment of mineral nutrient absorption in M. oryzae-infected rice plants.
Five types of kitchen waste (KW) from China – including hop pot (HP), fast food (FF), Hebei cuisine (HC), university canteen (UC), and other mixed KW (Other) – were investigated as feedstock for potential biogas and methane production. The biodegradability of KW was measured using batch anaerobic digestion (AD) tests and feedstock at an inoculum ratio (F/I) of 0.5. Gompertz and Cone models were used to determine the kinetic parameters of KW degradation, biogas, and methane production. Results showed that HP had the highest lag phase time of 5.46 days. Methane production varies with different sources of KW. HP had the highest methane yield of 363.9 mL/g-VSadded as compared to a sample of FF (334.8 mL/g-VSadded), other ( 278.5 mL/g-VSadded), UC (239.2 mL/g-VSadded), and HC (236.0 mL/g-VSadded). The biodegradability of KW ranged from 39.5% to 50.4%. During the AD process a certain amount of floating brown particles (FBP) were formed, which may be the main inhibiting factor of methane production. Analysis of ¹³C NMR and FTIR revealed that the main component of FBP was calcium stearate. The formation mechanism of calcium stearate may contribute to the relatively high lipid content (18.6% to 30.9%) of the KW sample, which subsequently resulted in over-accumulation of long-chain fatty acids (LCFAs) and reaction with Ca²⁺. Using lipid-rich substrates as feedstock may be an efficient approach to adding Ca²⁺ artificially for reducing the inhibition of LCFAs.
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