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The extracellular domain of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) may function as a soluble cytokine to selectively kill various cancer cells without toxicity to most normal cells. We used a high-biosafety plasmid pVAX1 as a vector and constructed a recombinant plasmid expressing the extracellular domain (95-281 aa) of human TRAIL fused with signal peptides of human IgGγ, designated as pVAX-sT. Transduction of human BEL7402 liver cancer cells with pVAX-sT led to high levels of sTRAIL protein in the cell culture media and induced apoptosis. The therapeutic potential of pVAX-sT was then evaluated in the BEL7402 transplanted naked mouse model. Subsequent intratumoral administration of naked pVAX-sT resulted in the expression of soluble TRAIL in the sera and the tumor site, as well as effective suppression of tumor growth, with no toxicity to liver. In conclusion, the successful inhibition of liver cancer growth and the absence of detectable toxicity suggest that pVAX-sT could be useful in the gene therapy of liver cancer.
Flower colour polymorphism is attributed to pollinator or non-pollinator mediated selection. Geranium nepalense has common white morph and very rare pink morph. We compared pollinator visitation frequencies, temperature and soil moisture between two morphs in the mixed morph population. We also compared morph ratio and reproductive success between white and pink flower individuals. Our results indicated that no visitor groups were different between two colour morphs. But visitor groups differed in visits between two years. Halictidae preferred pink morph in the year of 2012 but showed no discrimination in 2014, whereas Syrphidae preferred white morph in 2014 but no discrimination in 2012. Overall, pink morph produced more seeds than white morph, but exhibited variation between two years. However, visitor discrimination was not the main cause of the difference in female fitness. Soil moisture was not different between two colour morphs. Temperature of white morph was lower than pink in evening but not different in morning and noon. The results indicated that non-pollinator factors may exert the selective pressure to maintain the flower colour polymorphism in this species. Although pollinators did not exert selection on the flower colour polymorphism, we suggest that they provided potential pollination environment of fluctuating selection to drive flower colour evolution if visitors were limited.
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