Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 11

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
Leaves and internodes from Stevia rebaudiana Bertoni plants growing in different conditions were used for transformation with two strains of Agrobacterium rhizogenes: ATCC 15384 and LBA 9402. Hairy roots formation was observed and the percentage of the transformed explants depended on the type of explant, time of inoculation and inoculum concentration. Inoculation of explants from ex vitro and in vitro plants with LBA 9402 strain led to higher efficiency of transformation than inoculation with ATCC 15384 strain. Growth rate of hairy roots in liquid culture was assessed under light and dark conditions. It was found that the growth of hairy roots decreased significantly under light conditions. Transformation of hairy roots growing in different culture conditions was confirmed at the molecular level using PCR method with primers constructed against rolB and rolC genes from A. rhizogenes.
Changes in malate concentration and activity of NADP-dependent malic enzyme were observed as the effect of Botrytis cinerea infection of C₃ or CAM-performing Mesembryanthemum crystallinum plants. Biotic stress applied on C₃ plants led to increase in malate concentration during the night and in consequence it led to increase in Δ-malate (day/night fluctuations) in infected leaves on the 2nd day post infection (dpi). It corresponded with induction of additional isoform of NADP-malic enzyme (NADP-ME3). On the contrary, CAM-performing M. crystallinum plants exhibited decrease in malate concentration and decay in its diurnal fluctuations as a reaction to B. cinerea infection. This correlated with significant decrease in activities of NADP-malic enzyme isoforms on the 2nd dpi as well as no fluctuations in their activities on the 9th dpi. Presented results point out to differences between C₃ and CAM plants in the direction of changes in primary metabolism providing energy, reducing equivalents and carbon skeletons for defense responses to halt the pathogen growth.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.