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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Genetic diversity of Brassica rapa germplasm of Khyber Pakhtunkhwa Pakistan revealed by molecular markers

100%
Ali N., Ali S., Khan N. U., Jan S. A., Rabbani M. A., Hussain I.
Acta Scientiarum Polonorum. Hortorum Cultus
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2019
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tom 18
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nr 6
A total of 96 indigenous Brassica rapa accessions were collected from different locations of Khyber Pakhtunkhwa, Pakistan. Simple Sequence Repeats (SSR) markers were used to identify the most diverse genotypes among the collected lots. Twenty six (26) different SSR primers were used for (genetic) variability among collected genotypes. These primers were selected from literature based on their previous results. These primers produced 135 scorable bands of which 75 were polymorphic, with an average of 55.5% polymorphic loci, and reflected the broader genetic background of the collected genotypes. An average 2.88 polymorphic bands with an average PIC value of 0.49 was recorded. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) divided all genotypes into three main groups. Group one contained three clusters, while group two and three had four and two clusters each. Based on the UPGMA dendrogram, genotypes collected from Kohat, Bannu, Swat and Haripur showed considerable amount of variation. From the present study, it is concluded that SSR markers can be proved as the best tool for the genetic variability of other local and exotic B. rapa genotypes.
2
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Effect of chilling exposure and vapor heat treatment duration on the quality of sweet orange during simulated marketing

100%
Hussain I., Rab A., Khan N. U., Sajid M., Ali N., Khan S. M., Ali S.
Acta Scientiarum Polonorum. Hortorum Cultus
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2017
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tom 16
|
nr 5
Sweet orange fruits were exposed to vapor heat treatment (50°C) in water bath for 0, 5, 10, 15 and 20 min in plastic covered structure. The data were recorded on different physico chemical factors immediately after the storage and after seven days simulated marketing under ambient condition (20°C). Low temperature storage enhanced weight loss, surface pitting, disease incidence, total soluble solids accumulation, TSS/Acid ratio but decreased reducing sugars, acidity and ascorbic acid content. Chilling exposure up to 45 days had no significant effect on weight loss and TSS. However, increased weight loss (2.63%), TSS (11.75), TSS/Acid ratio (8.45 ºBrix), disease incidence (8.93%) and lowest reducing sugars (3.90) were noted in sweet orange exposed to chilling temperature for 75 days. Among the VHT durations, the highest weight loss (2.29%) was found in VHT for 0 min while the highest TSS (11.81 ºBrix), TSS/Acid ratio (8.10) and disease incidence (6.22%) and least reducing sugars (4.12%) were found in VHT 20 for min. Vapor heat treatment ranging from 5–10 min resulted in lowest weight loss (1.79%), TSS (10.81 ºBrix) TSS/Acid ratio (7.33), disease incidence (1.00%) and highest reducing sugars (4.75%) in sweet orange fruits. However, non-reducing sugars were least affected by both LTSs and VHTs. It is concluded that the chilling exposure (5°C) beyond 45 days aggravated the decline of fruit physio-chemical quality characteristics. Whereas, VHT with 5–10 min maintained the sweet orange fruit quality during simulated marketing; however, VHT of 15–20 min adversely affected the sweet orange fruit quality attributes.
3
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Peach antioxidant and phenolic activities influenced by the application of 1-methylcyclopropene (1-MCP) at post-harvest

84%
Shah S. T., Sajid M., Khan N. U., Rab A., Amin N. U., Arif M., Haleema B., Saeed S.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2019
|
tom 18
|
nr 2
Early maturing peach (Prunus persica) cultivars can fetch good market value but face a lot of post-harvest problems that lead to the post-harvest losses. The 1-methylcyclopropene (1-MCP) can provide new insights into plant ethylene responses and extend the shelf life and quality of fruits. Therefore, fruits of peach cultivar ‘Early Grand’ were dipped in various concentrations of 1-MCP (0, 0.3, 0.6 and 0.9 µg l–1), stored for 40 days at 8 ±2°C with 50% relative humidity and analyzed the fruits for physicochemical attributes at 10 days interval. The highest concentration of 1-MCP at 0.9 µg l–1 significantly improved the activity of antioxidants, catalase, free radical scavenging assay and total phenols. However, the peach fruits treated with 1MCP at 0.6 µgL–1 was effective in retaining the ascorbic acid, lowering the weight loss and fruit decay. Therefore, peach fruits can be treated with 1-MCP (0.6 µg l–1) solution for prolonging its shelf life up to 40 days under low temperature.
4

Polymorphisms in JAK2 gene are associated with production traits and mastitis resistance in dairy cattle

84%
Ali N., Niaz S., Khan N. U., Gohar A., Khattak I., Dong Y., Khattak T., Ahmad I., Wang Y., Usman T.
Annals of Animal Science
|
2020
|
tom 20
|
nr 2
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