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1

In vitro biotechnological approaches on Vanilla planifolia Andrews: advancements and opportunities

100%
Gantait S., Kundu S.
Acta Physiologiae Plantarum
|
2017
|
tom 39
|
nr 09
2

In vitro direct rhizogenesis from Gerbera jamesonii Bolus leaf

100%
Gantait S., Sinniah U. R.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 11
The present report describes an original protocol for in vitro direct induction of roots from leaf explants of gerbera for the first time. Since gerbera has immense potential as a premium cut-flower, the major attempts were made on in vitro mass propagation chiefly through in vitro multiple shoot proliferation or callus regeneration. Nevertheless, rhizogenesis could be impending an unattempted method with its yet-to-be known advantages. In our study, the optimum conditions for direct root induction from leaf explants were assessed employing tissue culture technique. Leaves were inoculated to MS medium containing no or variable auxin sources and concentrations namely, 2,4- dichlorophenoxyacetic acid, indole-3-acetic acid (IAA), indole-3-butyric acid or α-naphthaleneacetic acid for root induction. It was evident that the maximum root induction (with a frequency of 92.6 %) occurred on MS media fortified with 1.5 mg l⁻¹ IAA, wherein root induction was observed as early as 11 days of culture and an average of ~19 roots with ~13 mm length was obtained from 4 cm² leaf segment after 45 days of culture. Stereo microscopic observation revealed the induction of roots and gradual developmental stages of rhizogenesis. The efficiency of direct root induction without any interim growth stages (such as, callus or shoots) in our study offers a reproducible system that could provide a model protocol for more comprehensive developmental studies on root growth.
3

Cryopreservation of immature Parkia speciosa Hassk. zygotic embryonic axes following desiccation or exposure to vitrification solution

100%
Sinniah U. R., Gantait S.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 08
This work reports on the cryopreservation of immature zygotic embryonic axes (EA) of petai (Parkia speciosa Hassk.) for the first time. Two cryopreservation protocols, namely desiccation and vitrification method were tested individually using excised EA. Desiccation of EA to lower moisture content (MC) reduced the survival percentage but a drastic decline in survival percentage (*20 %) was recorded at 16 % MC prior to exposure to LN, rendering the EA to be sensitive to desiccation. Cryopreservation of EA after desiccation, irrespective of the MC, did not result in any survival. On the other hand, post-cryopreservation survival was obtained when the EA were exposed to plant vitrification solution-2 (PVS2) for 75–105 min. The best results were obtained when the EA were exposed to PVS2 for 90 min with an average recovery of 55.5 %. EA recovery into whole plantlets was obtained when the EA were cultured on MS medium supplemented with 2 gl-1 activated charcoal and 0.1 mgl-1 of the plant growth regulators α-naphthalene acetic acid, 6-benzylaminopurine and gibberellin A3, each. EA, exposed for less than 75 min and more than 105 min to PVS2, did not show any survival after cryopreservation. The optimization of exposure time is necessary to increase survival. This study has shown that the employment of suitable method is important for conservation using cryopreservation.
4

Synthetic seed production of medicinal plants: a review on influence of explants, encapsulation agent and matrix

81%
Gantait S., Kundu S., Ali N., Sahu N. C.
Acta Physiologiae Plantarum
|
2015
|
tom 37
|
nr 05
The present review illustrates the implementation of synthetic seed technology for mass propagation and short-term storage of several medicinal plants, popularly grown throughout the world. Biotechnology-based research with special reference to in vitro plant cell and tissue culture intervention created a new outlook in terms of mass propagation, germplasm storage and cryoconservation, production of secondary metabolites as well as genetic transformation. Synthetic seed technology involving alginate encapsulation of in vitro or in vivo generated explants proved to be a competent system to deal with multiplication, storage and exchange of seedless medicinal plants having traits of choice that are intricate to propagate via conventional approach. Nevertheless, optimization of production, storage and exchange of synthetic seeds are influenced by several factors. Manipulation of those factors such as explant selection, encapsulating agent and matrix determined the success of synthetic seed technology in medicinal plants. The present review elucidates an outline of past progress, present status and future prospects of synthetic seed technology intervention in medicinal plants with special emphasis on the factors which determine the success of this technology.
5

In vitro developmental study of oil palm (Elaeis guineensis Jacq.) polyembryoids from cell suspension using scanning electron microscopy

51%
Palanyandy S. R., Suranthran P., Gantait S., Sinniah U. R., Subramaniam S., Aziz M. A., Alwee S. S. R. S., Roowi S. H.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 05
In the present study, we report the in vitro development of polyembryoids with identification of a definite stage that can be used for subsequent uniform plantlet regeneration in oil palm (Elaeis guineensis Jacq.). Induction and maturation of polyembryoids was accomplished when cell suspension culture was transferred in MS (Murashige and Skoog, Physiol Plant 15:473–497, 1962) semisolid medium consisting of 30 g L-1 sucrose and 3.5 g L-1 gelrite devoid of any plant growth regulator. Growth and development of cell suspension culture into polyembryoids were assessed by stereo and scanning electron microscopy (SEM) to identify the sequential events as well as the differentiation that occur during each stage. Observations on the differentiation symptoms showed that the embryos pass through distinct morphological characteristics indicating distinctively varied stages. SEM observations indicated the development of extracellular network at an early stage of differentiation and acts as the structural marker of differentiation leading to the development of polyembryoids via formation of globular proembryo and haustorium. Eventually, a specific developmental stage comprising haustorium and torpedo-shaped structure was identified, for conservation, regeneration or multiplication, based on the embryogenic competence.
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