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Rapid DNA extraction for screening soil filamentous fungi using PCR amplification

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Plaza G. A., Upchurch R., Brigmon R. L., Whitman W. B., Ulfig K.

Polish Journal of Environmental Studies

2004

tom 13

nr 3

A simple and rapid procedure for efficiently isolating fungi DNA suitable for use as a template for PCR amplification and other molecular assays is described. The main advantages of the method are: (1) the mycelium is directly recovered from Petri-dish cultures; (2) the technique is rapid and relatively easy to perform , and (3) it allows for processing of around 50 samples during a single day; (4) it is inexpensive; (5) the quality and quantity of DNA obtained are suitable for molecular assays; (6) it can be applied to filamentous fungi from soil as well as from a fungi from other environmental sources; and (7) it does not require the use of expensive and specialized equipment or hazardous reagents.

Ograniczanie wyników

1 Polish Journal of Environmental Studies

1 Brigmon R. L.

1 Plaza G. A.

1 Ulfig K.

1 Upchurch R.

1 Whitman W. B.

1 2004

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Rapid DNA extraction for screening soil filamentous fungi using PCR amplification